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Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media.
Sci Data 2019; 6(1):322SD

Abstract

Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.

Authors+Show Affiliations

Department of Bioengineering, University of California, San Diego, La Jolla, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, 92093, United States of America. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA. Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA, 92093, USA.Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens, Lyngby, Denmark.Department of Bioengineering, University of California, San Diego, La Jolla, USA. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens, Lyngby, Denmark.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA. jpogliano@ucsd.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

31848353

Citation

Rajput, Akanksha, et al. "Profiling the Effect of Nafcillin On HA-MRSA D712 Using Bacteriological and Physiological Media." Scientific Data, vol. 6, no. 1, 2019, p. 322.
Rajput A, Poudel S, Tsunemoto H, et al. Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. Sci Data. 2019;6(1):322.
Rajput, A., Poudel, S., Tsunemoto, H., Meehan, M., Szubin, R., Olson, C. A., ... Pogliano, J. (2019). Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. Scientific Data, 6(1), p. 322. doi:10.1038/s41597-019-0331-z.
Rajput A, et al. Profiling the Effect of Nafcillin On HA-MRSA D712 Using Bacteriological and Physiological Media. Sci Data. 2019 12 17;6(1):322. PubMed PMID: 31848353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. AU - Rajput,Akanksha, AU - Poudel,Saugat, AU - Tsunemoto,Hannah, AU - Meehan,Michael, AU - Szubin,Richard, AU - Olson,Connor A, AU - Lamsa,Anne, AU - Seif,Yara, AU - Dillon,Nicholas, AU - Vrbanac,Alison, AU - Sugie,Joseph, AU - Dahesh,Samira, AU - Monk,Jonathan M, AU - Dorrestein,Pieter C, AU - Knight,Rob, AU - Nizet,Victor, AU - Palsson,Bernhard O, AU - Feist,Adam M, AU - Pogliano,Joe, Y1 - 2019/12/17/ PY - 2019/07/30/received PY - 2019/11/07/accepted PY - 2019/12/19/entrez PY - 2019/12/19/pubmed PY - 2019/12/19/medline SP - 322 EP - 322 JF - Scientific data JO - Sci Data VL - 6 IS - 1 N2 - Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media. SN - 2052-4463 UR - https://www.unboundmedicine.com/medline/citation/31848353/Profiling_the_effect_of_nafcillin_on_HA_MRSA_D712_using_bacteriological_and_physiological_media_ L2 - http://dx.doi.org/10.1038/s41597-019-0331-z DB - PRIME DP - Unbound Medicine ER -