Tags

Type your tag names separated by a space and hit enter

Effects of a probiotic intervention on Escherichia coli and high-fat diet-induced intestinal microbiota imbalance.
Appl Microbiol Biotechnol. 2020 Feb; 104(3):1243-1257.AM

Abstract

Obesity is often associated with intestinal microbiota imbalance and increased Gram-negative bacteria characterized by higher endotoxin levels. Therefore, a study on the joint effects of a high-fat diet and Gram-negative bacteria prechallenge might provide further information on the interactive effects of intestinal microbiota and obesity as well as the effects of a probiotic intervention on these processes. This study focused on the joint effects of a high-fat diet and Escherichia coli on mouse inflammatory cytokines, intestinal microbiota, hepatic pathological changes, and the alleviating capacity of probiotics (Lactobacillus plantarum, Bifidobacterium breve, and Lactobacillus fermentum). Our results showed that E. coli administration and the high-fat diet exacerbated the inflammatory syndrome by increasing the visceral fat content, the inflammatory cell infiltration, and intestinal microbiota disorder in mice. E. coli administration caused a decrease in short-chain fatty acids in mouse feces, and probiotics effectively improved this phenomenon. Compound probiotic intervention reduced LPS and IL-1β levels, while increased IL-10 levels in mice improved the degeneration and inflammatory infiltration of mouse liver cells. The intestinal microbiota showed great differences at 3 weeks and 6 weeks post-administration. High fat and E. coli alone or in combination caused intestinal microbiota disorder, with increased harmful bacteria, and the probiotics effectively improved the intestinal flora structure and increased the fecal short-chain fatty acid (SCFA) content. In conclusion, a high-fat diet and Gram-negative bacteria challenge exacerbated the inflammatory syndrome, which can be alleviated by compound probiotic intervention.

Authors+Show Affiliations

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China. xiuliangli888@163.com. Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin, 150080, China. xiuliangli888@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31853561

Citation

Sun, Qingshen, et al. "Effects of a Probiotic Intervention On Escherichia Coli and High-fat Diet-induced Intestinal Microbiota Imbalance." Applied Microbiology and Biotechnology, vol. 104, no. 3, 2020, pp. 1243-1257.
Sun Q, Zhang S, Liu X, et al. Effects of a probiotic intervention on Escherichia coli and high-fat diet-induced intestinal microbiota imbalance. Appl Microbiol Biotechnol. 2020;104(3):1243-1257.
Sun, Q., Zhang, S., Liu, X., Huo, Y., Su, B., & Li, X. (2020). Effects of a probiotic intervention on Escherichia coli and high-fat diet-induced intestinal microbiota imbalance. Applied Microbiology and Biotechnology, 104(3), 1243-1257. https://doi.org/10.1007/s00253-019-10304-4
Sun Q, et al. Effects of a Probiotic Intervention On Escherichia Coli and High-fat Diet-induced Intestinal Microbiota Imbalance. Appl Microbiol Biotechnol. 2020;104(3):1243-1257. PubMed PMID: 31853561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a probiotic intervention on Escherichia coli and high-fat diet-induced intestinal microbiota imbalance. AU - Sun,Qingshen, AU - Zhang,Song, AU - Liu,Xinyang, AU - Huo,Yuting, AU - Su,Bin, AU - Li,Xiuliang, Y1 - 2019/12/18/ PY - 2019/08/26/received PY - 2019/12/08/accepted PY - 2019/11/14/revised PY - 2019/12/20/pubmed PY - 2020/10/3/medline PY - 2019/12/20/entrez KW - Compound probiotics KW - Escherichia coli KW - High-fat diet KW - Inflammation KW - Intestinal microbiota SP - 1243 EP - 1257 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 104 IS - 3 N2 - Obesity is often associated with intestinal microbiota imbalance and increased Gram-negative bacteria characterized by higher endotoxin levels. Therefore, a study on the joint effects of a high-fat diet and Gram-negative bacteria prechallenge might provide further information on the interactive effects of intestinal microbiota and obesity as well as the effects of a probiotic intervention on these processes. This study focused on the joint effects of a high-fat diet and Escherichia coli on mouse inflammatory cytokines, intestinal microbiota, hepatic pathological changes, and the alleviating capacity of probiotics (Lactobacillus plantarum, Bifidobacterium breve, and Lactobacillus fermentum). Our results showed that E. coli administration and the high-fat diet exacerbated the inflammatory syndrome by increasing the visceral fat content, the inflammatory cell infiltration, and intestinal microbiota disorder in mice. E. coli administration caused a decrease in short-chain fatty acids in mouse feces, and probiotics effectively improved this phenomenon. Compound probiotic intervention reduced LPS and IL-1β levels, while increased IL-10 levels in mice improved the degeneration and inflammatory infiltration of mouse liver cells. The intestinal microbiota showed great differences at 3 weeks and 6 weeks post-administration. High fat and E. coli alone or in combination caused intestinal microbiota disorder, with increased harmful bacteria, and the probiotics effectively improved the intestinal flora structure and increased the fecal short-chain fatty acid (SCFA) content. In conclusion, a high-fat diet and Gram-negative bacteria challenge exacerbated the inflammatory syndrome, which can be alleviated by compound probiotic intervention. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/31853561/Effects_of_a_probiotic_intervention_on_Escherichia_coli_and_high_fat_diet_induced_intestinal_microbiota_imbalance_ L2 - https://dx.doi.org/10.1007/s00253-019-10304-4 DB - PRIME DP - Unbound Medicine ER -