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"Differential responsiveness to BMP9 between patent and fused suture progenitor cells from craniosynostosis patients."
Plast Reconstr Surg. 2019 Dec 17 [Online ahead of print]PR

Abstract

BACKGROUND

Several studies have verified that BMPs may be involved in the development of craniosynostosis; little attention has been focused on the role of BMP9 in cranial suture biology. BMP9 is the most osteogenic BMP and promotes the osteoblastic differentiation of mesenchymal stem cells. We investigated the role of BMP9 in suture progenitor cells (SuPs).

METHODS

We isolated and cultured prematurely fused (FSuPs) and internal control patent (PSuPs) suture progenitor cells from patients with nonsyndromic craniosynostosis. Overexpression of BMP9 was mediated by adenoviral vectors. Osteoblast and osteoclast differentiation-related markers were evaluated by staining techniques and TqPCR analysis. In vivo analysis of BMP9-induced SuPs osteogenesis was performed in an ectopic bone formation model.

RESULTS

We demonstrated that the prematurely fused sutures have a higher endogenous expression of the osteogenic differentiation-related genes than patent sutures, while the same pattern of gene expression exists between FSuPs and PSuPs. Importantly, both PSuPs and FSuPs undergo osteogenic differentiation and express multiple lineage regulators and NELL-1 upon BMP9 stimulation, while FSuPs have a higher basal osteogenic potential than PSuPs. BMP9 regulates the expression of osteoclast differentiation-related genes in SuPs. Forced BMP9 expression enhances the mineralization and maturity of ectopic bone formation of SuPs implanted in vivo.

CONCLUSIONS

Our findings suggest that FSuPs have an elevated osteogenic potential. BMP9 could regulate the expression of multiple osteoblast and osteoclast differentiation-related genes, as well as NELL1 in both SuPs, indicating that BMP9 may play a role in craniosynostosis.

Authors+Show Affiliations

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China. Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA. Department of Surgery, Laboratory of Craniofacial Biology and Development, Section of Plastic Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31860505

Citation

Song, Dongzhe, et al. ""Differential Responsiveness to BMP9 Between Patent and Fused Suture Progenitor Cells From Craniosynostosis Patients."" Plastic and Reconstructive Surgery, 2019.
Song D, Huang S, Zhang L, et al. "Differential responsiveness to BMP9 between patent and fused suture progenitor cells from craniosynostosis patients." Plast Reconstr Surg. 2019.
Song, D., Huang, S., Zhang, L., Liu, W., Huang, B., Feng, Y., Liu, B., He, T. C., Huang, D., & Reid, R. R. (2019). "Differential responsiveness to BMP9 between patent and fused suture progenitor cells from craniosynostosis patients." Plastic and Reconstructive Surgery. https://doi.org/10.1097/PRS.0000000000006597
Song D, et al. "Differential Responsiveness to BMP9 Between Patent and Fused Suture Progenitor Cells From Craniosynostosis Patients.". Plast Reconstr Surg. 2019 Dec 17; PubMed PMID: 31860505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - "Differential responsiveness to BMP9 between patent and fused suture progenitor cells from craniosynostosis patients." AU - Song,Dongzhe, AU - Huang,Shifeng, AU - Zhang,Linghuan, AU - Liu,Wei, AU - Huang,Bo, AU - Feng,Yixiao, AU - Liu,Bo, AU - He,Tong-Chuan, AU - Huang,Dingming, AU - Reid,Russell R, Y1 - 2019/12/17/ PY - 2019/12/21/entrez PY - 2019/12/21/pubmed PY - 2019/12/21/medline JF - Plastic and reconstructive surgery JO - Plast. Reconstr. Surg. N2 - BACKGROUND: Several studies have verified that BMPs may be involved in the development of craniosynostosis; little attention has been focused on the role of BMP9 in cranial suture biology. BMP9 is the most osteogenic BMP and promotes the osteoblastic differentiation of mesenchymal stem cells. We investigated the role of BMP9 in suture progenitor cells (SuPs). METHODS: We isolated and cultured prematurely fused (FSuPs) and internal control patent (PSuPs) suture progenitor cells from patients with nonsyndromic craniosynostosis. Overexpression of BMP9 was mediated by adenoviral vectors. Osteoblast and osteoclast differentiation-related markers were evaluated by staining techniques and TqPCR analysis. In vivo analysis of BMP9-induced SuPs osteogenesis was performed in an ectopic bone formation model. RESULTS: We demonstrated that the prematurely fused sutures have a higher endogenous expression of the osteogenic differentiation-related genes than patent sutures, while the same pattern of gene expression exists between FSuPs and PSuPs. Importantly, both PSuPs and FSuPs undergo osteogenic differentiation and express multiple lineage regulators and NELL-1 upon BMP9 stimulation, while FSuPs have a higher basal osteogenic potential than PSuPs. BMP9 regulates the expression of osteoclast differentiation-related genes in SuPs. Forced BMP9 expression enhances the mineralization and maturity of ectopic bone formation of SuPs implanted in vivo. CONCLUSIONS: Our findings suggest that FSuPs have an elevated osteogenic potential. BMP9 could regulate the expression of multiple osteoblast and osteoclast differentiation-related genes, as well as NELL1 in both SuPs, indicating that BMP9 may play a role in craniosynostosis. SN - 1529-4242 UR - https://www.unboundmedicine.com/medline/citation/31860505/"Differential_responsiveness_to_BMP9_between_patent_and_fused_suture_progenitor_cells_from_craniosynostosis_patients_" L2 - http://Insights.ovid.com/pubmed?pmid=31860505 DB - PRIME DP - Unbound Medicine ER -
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