TY - JOUR T1 - Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia. AU - Longo,Sébastien, AU - Sibat,Manoella, AU - Viallon,Jérôme, AU - Darius,Hélène Taiana, AU - Hess,Philipp, AU - Chinain,Mireille, Y1 - 2019/12/17/ PY - 2019/11/26/received PY - 2019/12/11/revised PY - 2019/12/12/accepted PY - 2019/12/22/entrez PY - 2019/12/22/pubmed PY - 2020/10/27/medline KW - 44-methylgambierone KW - CBA-N2a KW - Gambierdiscus polynesiensis KW - LC-MS/MS KW - ciguatera KW - ciguatoxins KW - gambierone KW - toxin profiles JF - Toxins JO - Toxins (Basel) VL - 11 IS - 12 N2 - Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography-tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell-1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/31861242/Intraspecific_Variability_in_the_Toxin_Production_and_Toxin_Profiles_of_In_Vitro_Cultures_of_Gambierdiscus_polynesiensis__Dinophyceae__from_French_Polynesia_ DB - PRIME DP - Unbound Medicine ER -