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Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis.
Toxicol Lett. 2020 Mar 15; 321:61-68.TL

Abstract

T-2 toxin and deoxynivalenol (DON) are two representative mycotoxins that are commonly found in cereals and agricultural products. As T-2 toxin and DON are considered the cause of Kashin-Beck disease, a special osteoarticular disease, chondrocytes would be a vital target site for these toxins. To fully understand the toxicity effects of T-2 toxin and DON on chondrocytes, the present study investigated and compared the gene expression profiles and underlying mechanisms of T-2 toxin and DON on cultured human chondrocytes by microarray and bioinformatics analysis. Normal human chondrocytes were treated with T-2 toxin at 0.01 μg/ml and DON at 1.0 μg/ml for 72 h and analyzed by microarray using Affymetrix Human Gene Chip. Comprehensive analysis, including gene ontology, pathways and gene-gene networks was performed to identify the crucial gene functions, related signal pathways and key genes. A total of 175 and 237 differentially expressed genes were identified in human chondrocytes for T-2 toxin and DON treatment, respectively. Of these, 47 had the same expression tendencies in the two groups. The protein-protein interaction network analysis showed that the 10 hub genes were different between the two groups. Our results provide a comprehensive understanding of the toxic mechanism of T-2 toxin and DON on human chondrocytes and suggest that although T-2 toxin and DON showed some similar toxic mechanisms in human chondrocytes, they also had different toxic characteristics.

Authors+Show Affiliations

School of Nursing, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China; School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Collaborative Innovation Center of Endemic Diseases and Health Promotion in Silk Road Region, Xi'an, Shaanxi, PR China.Yanan University Affiliated Hospital, Yanan, Shaanxi, PR China.Hong Hui Hospital, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Collaborative Innovation Center of Endemic Diseases and Health Promotion in Silk Road Region, Xi'an, Shaanxi, PR China.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Collaborative Innovation Center of Endemic Diseases and Health Promotion in Silk Road Region, Xi'an, Shaanxi, PR China. Electronic address: guox@mail.xjtu.edu.cn.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

31863870

Citation

Yang, Lei, et al. "Comparison of the Toxic Mechanism of T-2 Toxin and Deoxynivalenol On Human Chondrocytes By Microarray and Bioinformatics Analysis." Toxicology Letters, vol. 321, 2020, pp. 61-68.
Yang L, Wang S, Zhao G, et al. Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis. Toxicol Lett. 2020;321:61-68.
Yang, L., Wang, S., Zhao, G., Wang, X., & Guo, X. (2020). Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis. Toxicology Letters, 321, 61-68. https://doi.org/10.1016/j.toxlet.2019.12.024
Yang L, et al. Comparison of the Toxic Mechanism of T-2 Toxin and Deoxynivalenol On Human Chondrocytes By Microarray and Bioinformatics Analysis. Toxicol Lett. 2020 Mar 15;321:61-68. PubMed PMID: 31863870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis. AU - Yang,Lei, AU - Wang,Suiqin, AU - Zhao,Guanghui, AU - Wang,Xi, AU - Guo,Xiong, Y1 - 2019/12/19/ PY - 2019/08/22/received PY - 2019/11/06/revised PY - 2019/12/17/accepted PY - 2019/12/22/pubmed PY - 2020/1/28/medline PY - 2019/12/22/entrez KW - Bioinformatics analysis KW - Chondrocytes KW - Deoxynivalenol KW - Gene expression KW - T-2 toxin SP - 61 EP - 68 JF - Toxicology letters JO - Toxicol. Lett. VL - 321 N2 - T-2 toxin and deoxynivalenol (DON) are two representative mycotoxins that are commonly found in cereals and agricultural products. As T-2 toxin and DON are considered the cause of Kashin-Beck disease, a special osteoarticular disease, chondrocytes would be a vital target site for these toxins. To fully understand the toxicity effects of T-2 toxin and DON on chondrocytes, the present study investigated and compared the gene expression profiles and underlying mechanisms of T-2 toxin and DON on cultured human chondrocytes by microarray and bioinformatics analysis. Normal human chondrocytes were treated with T-2 toxin at 0.01 μg/ml and DON at 1.0 μg/ml for 72 h and analyzed by microarray using Affymetrix Human Gene Chip. Comprehensive analysis, including gene ontology, pathways and gene-gene networks was performed to identify the crucial gene functions, related signal pathways and key genes. A total of 175 and 237 differentially expressed genes were identified in human chondrocytes for T-2 toxin and DON treatment, respectively. Of these, 47 had the same expression tendencies in the two groups. The protein-protein interaction network analysis showed that the 10 hub genes were different between the two groups. Our results provide a comprehensive understanding of the toxic mechanism of T-2 toxin and DON on human chondrocytes and suggest that although T-2 toxin and DON showed some similar toxic mechanisms in human chondrocytes, they also had different toxic characteristics. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/31863870/Comparison_of_the_toxic_mechanism_of_T-2_toxin_and_deoxynivalenol_on_human_chondrocytes_by_microarray_and_bioinformatics_analysis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(19)30418-7 DB - PRIME DP - Unbound Medicine ER -