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Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows.
J Dairy Sci 2020; 103(2):1969-1981JD

Abstract

Eleven mid-lactation Holstein cows were milked twice daily during the first 2 experimental weeks. During wk 3 to 10, the cows were differentially milked: right quarters were milked thrice daily (3×) and left quarters were milked once daily (1×). During wk 11 to 14, all quarters were milked twice daily. After 4 wk of differential milking, the cows received daily i.m. injections of the dopamine antagonist domperidone (DOMP; 300 mg; n = 6) or of dimethyl sulfoxide as the control (CTL; n = 5) for 8 wk (wk 7-14). During the differential milking period (wk 3-6), milk production was greater for quarters milked 3× than for those milked 1× but did not differ between DOMP and CTL cows. During the differential milking + injection period (wk 7-10), milk production continued to differ according to milking frequency. However, DOMP injection did not have an effect or interact with milking frequency on milk production. During the injection period (wk 11-14), milk production remained greater in the quarters previously milked 3× and milk production increased in DOMP injected cows but not in CTL cows. Injections of DOMP increased prolactin concentration, which was greater in the serum of DOMP cows than in that of CTL cows during the differential milking + injection and the injection periods. The expression of genes that are directly related to milk synthesis (CSN2, LALBA, and ACACA) was greater in the 3× quarters than in the 1× quarters. In addition, DOMP increased CSN2 expression during the injection period. The expression of both isoforms of the PRLR gene was greater in the 3× quarters during the differential milking + injection and the injection periods. At the protein level, injections of DOMP tended to increase the number of long PRLR isoform during the differential milking + injection period. The number of short PRLR isoform was greater in the 1× quarters than in the 3× quarters during the differential milking, the differential milking + injection, and the injection periods. The total amount of STAT3 protein was greater in the 1× quarters during the differential milking and the differential milking + injection periods. The amount of phosphorylated STAT3 protein was greater in the 1× quarters during the differential milking period. The total amount of phosphorylated STAT5 protein was greater in the 3× quarters during the differential milking and the differential milking + injection periods. The results of this experiment support the hypothesis that the responsiveness of the mammary gland to PRL is modulated by milking frequency, although the underlying mechanism remains to be determined.

Authors+Show Affiliations

Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada J1M 0C8.Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada H9X 3V9.Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada J1M 0C8. Electronic address: pierre.lacasse@canada.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31864734

Citation

Toledo, I M., et al. "Effects of Milking Frequency and Domperidone Injections On Milk Production and Prolactin Signaling in the Mammary Gland of Dairy Cows." Journal of Dairy Science, vol. 103, no. 2, 2020, pp. 1969-1981.
Toledo IM, Zhao X, Lacasse P. Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows. J Dairy Sci. 2020;103(2):1969-1981.
Toledo, I. M., Zhao, X., & Lacasse, P. (2020). Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows. Journal of Dairy Science, 103(2), pp. 1969-1981. doi:10.3168/jds.2019-17330.
Toledo IM, Zhao X, Lacasse P. Effects of Milking Frequency and Domperidone Injections On Milk Production and Prolactin Signaling in the Mammary Gland of Dairy Cows. J Dairy Sci. 2020;103(2):1969-1981. PubMed PMID: 31864734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows. AU - Toledo,I M, AU - Zhao,X, AU - Lacasse,P, Y1 - 2019/12/19/ PY - 2019/07/25/received PY - 2019/10/29/accepted PY - 2019/12/23/pubmed PY - 2019/12/23/medline PY - 2019/12/23/entrez KW - STAT5 KW - differential milking KW - prolactin KW - serotonin SP - 1969 EP - 1981 JF - Journal of dairy science JO - J. Dairy Sci. VL - 103 IS - 2 N2 - Eleven mid-lactation Holstein cows were milked twice daily during the first 2 experimental weeks. During wk 3 to 10, the cows were differentially milked: right quarters were milked thrice daily (3×) and left quarters were milked once daily (1×). During wk 11 to 14, all quarters were milked twice daily. After 4 wk of differential milking, the cows received daily i.m. injections of the dopamine antagonist domperidone (DOMP; 300 mg; n = 6) or of dimethyl sulfoxide as the control (CTL; n = 5) for 8 wk (wk 7-14). During the differential milking period (wk 3-6), milk production was greater for quarters milked 3× than for those milked 1× but did not differ between DOMP and CTL cows. During the differential milking + injection period (wk 7-10), milk production continued to differ according to milking frequency. However, DOMP injection did not have an effect or interact with milking frequency on milk production. During the injection period (wk 11-14), milk production remained greater in the quarters previously milked 3× and milk production increased in DOMP injected cows but not in CTL cows. Injections of DOMP increased prolactin concentration, which was greater in the serum of DOMP cows than in that of CTL cows during the differential milking + injection and the injection periods. The expression of genes that are directly related to milk synthesis (CSN2, LALBA, and ACACA) was greater in the 3× quarters than in the 1× quarters. In addition, DOMP increased CSN2 expression during the injection period. The expression of both isoforms of the PRLR gene was greater in the 3× quarters during the differential milking + injection and the injection periods. At the protein level, injections of DOMP tended to increase the number of long PRLR isoform during the differential milking + injection period. The number of short PRLR isoform was greater in the 1× quarters than in the 3× quarters during the differential milking, the differential milking + injection, and the injection periods. The total amount of STAT3 protein was greater in the 1× quarters during the differential milking and the differential milking + injection periods. The amount of phosphorylated STAT3 protein was greater in the 1× quarters during the differential milking period. The total amount of phosphorylated STAT5 protein was greater in the 3× quarters during the differential milking and the differential milking + injection periods. The results of this experiment support the hypothesis that the responsiveness of the mammary gland to PRL is modulated by milking frequency, although the underlying mechanism remains to be determined. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/31864734/Effects_of_milking_frequency_and_domperidone_injections_on_milk_production_and_prolactin_signaling_in_the_mammary_gland_of_dairy_cows L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(19)31108-7 DB - PRIME DP - Unbound Medicine ER -