Tags

Type your tag names separated by a space and hit enter

Noninferiority study evaluating the efficacy of a teat disinfectant containing copper and zinc for prevention of naturally occurring intramammary infections in an automatic milking system.
J Dairy Sci 2020; 103(2):1776-1784JD

Abstract

The aim of this study was to demonstrate the noninferiority of a novel teat disinfectant based on copper and zinc (ZkinCu; Copper Andino, Santiago de Chile, Chile) compared with a previously proven glycolic acid active disinfectant (OceanBlu; DeLaval, Kansas City, MO) as a positive control, with respect to the incidence of new intramammary infections under natural challenge conditions on a commercial robotic dairy farm. This study was conducted in 6 robotic pens of approximately 60 milking cows each. The pens were randomly assigned to 1 of the 2 studied disinfectants. Throughout the 8 wk study, the same pre- and post-milking teat disinfectant was used in each pen. The same milking procedures were used in each robot throughout the study. Pre-milking hygiene consisted of applying the disinfectant (OceanBlu or ZkinCu) with the robotic arm. The same product was applied on the teats after milking. At the beginning of the study, all quarters of all study cows were sampled. In successive samplings (wk 2, 4, 6, and 8), composite milk samples were collected on farm to determine SCC. Once composite SCC results were available (2 d) and based on an SCC of ≥100,000 cells/mL, quarter milk samples underwent bacteriological culture. Clinical mastitis was identified by study personnel. Intramammary infection in biweekly quarter milk samples was determined based on composite SCC levels (≥100,000 cells/mL) and the presence of bacteria. A new IMI was defined as a quarter in which the organism isolated was not present in the previous bacteriological sample, or the previous composite SCC sample was <100,000 cells/mL. Clinical mastitis samples were also considered to be new IMI. The trial was designed as a positive control field trial, in which the objective was to show noninferiority of ZkinCu versus the control (OceanBlu). The overall crude incidences of new IMI for 2 wk at risk were 4.9 and 7.3% for the ZkinCu and OceanBlu groups, respectively. The predominant organisms recovered from quarters with new IMI were Streptococcus uberis, Corynebacterium spp., and coagulase-negative staphylococci in both the ZkinCu and OceanBlu groups. The risk of infection in the OceanBlu group was higher (β = 0.644; 95% confidence interval = 0.05-1.22). The interaction of treatment by week was not significant. The new IMI rate estimates (95% confidence interval) for ZkinCu and OceanBlu were 1.7% (0.8-2.5) and 3.2% (1.7-4.7), respectively. One novel aspect of this study is that it was one of the first commercial noninferiority trials to evaluate a new pre- and post-milking teat disinfectant in a dairy herd with an automatic milking system. The experimental teat disinfectant ZkinCu, evaluated in this field trial with naturally occurring IMI, showed noninferiority relative to the positive control for the prevention of new IMI. This study was conducted in a herd with an automatic milking system, and the results are applicable to herds with similar characteristics. Additional studies are needed to ensure reproducibility under different management conditions.

Authors+Show Affiliations

Universidad Nacional de Rio Cuarto CONICET, Ruta nacional 36 km 601, Río Cuarto 5800, Córdoba, Argentina.Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia 5090000, Chile.R&D Copper Andino S.A., Santiago de Chile 7501033, Chile.Lactodiagnostico Sur SRL, Olivos 1636, Buenos Aires, Argentina. Electronic address: martin.pol.mastitis@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31864745

Citation

Vissio, Claudina, et al. "Noninferiority Study Evaluating the Efficacy of a Teat Disinfectant Containing Copper and Zinc for Prevention of Naturally Occurring Intramammary Infections in an Automatic Milking System." Journal of Dairy Science, vol. 103, no. 2, 2020, pp. 1776-1784.
Vissio C, Mella A, Amestica L, et al. Noninferiority study evaluating the efficacy of a teat disinfectant containing copper and zinc for prevention of naturally occurring intramammary infections in an automatic milking system. J Dairy Sci. 2020;103(2):1776-1784.
Vissio, C., Mella, A., Amestica, L., & Pol, M. (2020). Noninferiority study evaluating the efficacy of a teat disinfectant containing copper and zinc for prevention of naturally occurring intramammary infections in an automatic milking system. Journal of Dairy Science, 103(2), pp. 1776-1784. doi:10.3168/jds.2018-16217.
Vissio C, et al. Noninferiority Study Evaluating the Efficacy of a Teat Disinfectant Containing Copper and Zinc for Prevention of Naturally Occurring Intramammary Infections in an Automatic Milking System. J Dairy Sci. 2020;103(2):1776-1784. PubMed PMID: 31864745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Noninferiority study evaluating the efficacy of a teat disinfectant containing copper and zinc for prevention of naturally occurring intramammary infections in an automatic milking system. AU - Vissio,Claudina, AU - Mella,Armin, AU - Amestica,Luis, AU - Pol,Martin, Y1 - 2019/12/19/ PY - 2018/12/27/received PY - 2019/07/17/accepted PY - 2019/12/23/pubmed PY - 2019/12/23/medline PY - 2019/12/23/entrez KW - automatic milking KW - intramammary infection KW - noninferiority trial KW - teat disinfectant SP - 1776 EP - 1784 JF - Journal of dairy science JO - J. Dairy Sci. VL - 103 IS - 2 N2 - The aim of this study was to demonstrate the noninferiority of a novel teat disinfectant based on copper and zinc (ZkinCu; Copper Andino, Santiago de Chile, Chile) compared with a previously proven glycolic acid active disinfectant (OceanBlu; DeLaval, Kansas City, MO) as a positive control, with respect to the incidence of new intramammary infections under natural challenge conditions on a commercial robotic dairy farm. This study was conducted in 6 robotic pens of approximately 60 milking cows each. The pens were randomly assigned to 1 of the 2 studied disinfectants. Throughout the 8 wk study, the same pre- and post-milking teat disinfectant was used in each pen. The same milking procedures were used in each robot throughout the study. Pre-milking hygiene consisted of applying the disinfectant (OceanBlu or ZkinCu) with the robotic arm. The same product was applied on the teats after milking. At the beginning of the study, all quarters of all study cows were sampled. In successive samplings (wk 2, 4, 6, and 8), composite milk samples were collected on farm to determine SCC. Once composite SCC results were available (2 d) and based on an SCC of ≥100,000 cells/mL, quarter milk samples underwent bacteriological culture. Clinical mastitis was identified by study personnel. Intramammary infection in biweekly quarter milk samples was determined based on composite SCC levels (≥100,000 cells/mL) and the presence of bacteria. A new IMI was defined as a quarter in which the organism isolated was not present in the previous bacteriological sample, or the previous composite SCC sample was <100,000 cells/mL. Clinical mastitis samples were also considered to be new IMI. The trial was designed as a positive control field trial, in which the objective was to show noninferiority of ZkinCu versus the control (OceanBlu). The overall crude incidences of new IMI for 2 wk at risk were 4.9 and 7.3% for the ZkinCu and OceanBlu groups, respectively. The predominant organisms recovered from quarters with new IMI were Streptococcus uberis, Corynebacterium spp., and coagulase-negative staphylococci in both the ZkinCu and OceanBlu groups. The risk of infection in the OceanBlu group was higher (β = 0.644; 95% confidence interval = 0.05-1.22). The interaction of treatment by week was not significant. The new IMI rate estimates (95% confidence interval) for ZkinCu and OceanBlu were 1.7% (0.8-2.5) and 3.2% (1.7-4.7), respectively. One novel aspect of this study is that it was one of the first commercial noninferiority trials to evaluate a new pre- and post-milking teat disinfectant in a dairy herd with an automatic milking system. The experimental teat disinfectant ZkinCu, evaluated in this field trial with naturally occurring IMI, showed noninferiority relative to the positive control for the prevention of new IMI. This study was conducted in a herd with an automatic milking system, and the results are applicable to herds with similar characteristics. Additional studies are needed to ensure reproducibility under different management conditions. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/31864745/Noninferiority_study_evaluating_the_efficacy_of_a_teat_disinfectant_containing_copper_and_zinc_for_prevention_of_naturally_occurring_intramammary_infections_in_an_automatic_milking_system L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(19)31095-1 DB - PRIME DP - Unbound Medicine ER -