Tags

Type your tag names separated by a space and hit enter

Label-free serum detection of Trichinella spiralis using surface-enhanced Raman spectroscopy combined with multivariate analysis.
Acta Trop 2019; 203:105314AT

Abstract

Based on blood serum surface-enhanced Raman spectroscopy (SERS) analysis, this paper proposed a simple and unlabeled non-invasive serum detection for T. spiralis infection. Serum samples were collected and analyzed from 40 rats at 0 days post infection (dpi) (normal rats), 19 uninfected rats, and 16 rats infected with T. spiralis at 28 dpi, using SERS measurements. Multivariate statistical techniques, such as linear discriminant analysis (LDA) and principal components analysis (PCA), were used to analyze and identify the obtained blood serum SERS spectra. The diagnosis algorithms, based on PCA-LDA, achieved a diagnostic sensitivity of 87.5%, a specificity of 94.7%, and an accuracy of 91.4% for separating the samples infected with T. spiralis from the control samples. This exploratory study demonstrated that colloidal Ag NPs-based SERS serum analysis technique combined with PCA-LDA has a great potential in improving the detection of T. spiralis infection and onsite screening.

Authors+Show Affiliations

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address: xuelin@jlu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31866336

Citation

Li, Jian, et al. "Label-free Serum Detection of Trichinella Spiralis Using Surface-enhanced Raman Spectroscopy Combined With Multivariate Analysis." Acta Tropica, vol. 203, 2019, p. 105314.
Li J, Ding J, Liu X, et al. Label-free serum detection of Trichinella spiralis using surface-enhanced Raman spectroscopy combined with multivariate analysis. Acta Trop. 2019;203:105314.
Li, J., Ding, J., Liu, X., Tang, B., Bai, X., Wang, Y., ... Wang, X. (2019). Label-free serum detection of Trichinella spiralis using surface-enhanced Raman spectroscopy combined with multivariate analysis. Acta Tropica, 203, p. 105314. doi:10.1016/j.actatropica.2019.105314.
Li J, et al. Label-free Serum Detection of Trichinella Spiralis Using Surface-enhanced Raman Spectroscopy Combined With Multivariate Analysis. Acta Trop. 2019 Dec 19;203:105314. PubMed PMID: 31866336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free serum detection of Trichinella spiralis using surface-enhanced Raman spectroscopy combined with multivariate analysis. AU - Li,Jian, AU - Ding,Jing, AU - Liu,Xiaolei, AU - Tang,Bin, AU - Bai,Xue, AU - Wang,Yang, AU - Li,Shicun, AU - Wang,Xuelin, Y1 - 2019/12/19/ PY - 2019/10/24/received PY - 2019/12/18/revised PY - 2019/12/18/accepted PY - 2019/12/24/pubmed PY - 2019/12/24/medline PY - 2019/12/24/entrez KW - Linear discriminant analysis (LDA) KW - Principal components analysis (PCA) KW - Serum detection KW - Surface-enhanced Raman spectroscopy (SERS) KW - Trichinella spiralis SP - 105314 EP - 105314 JF - Acta tropica JO - Acta Trop. VL - 203 N2 - Based on blood serum surface-enhanced Raman spectroscopy (SERS) analysis, this paper proposed a simple and unlabeled non-invasive serum detection for T. spiralis infection. Serum samples were collected and analyzed from 40 rats at 0 days post infection (dpi) (normal rats), 19 uninfected rats, and 16 rats infected with T. spiralis at 28 dpi, using SERS measurements. Multivariate statistical techniques, such as linear discriminant analysis (LDA) and principal components analysis (PCA), were used to analyze and identify the obtained blood serum SERS spectra. The diagnosis algorithms, based on PCA-LDA, achieved a diagnostic sensitivity of 87.5%, a specificity of 94.7%, and an accuracy of 91.4% for separating the samples infected with T. spiralis from the control samples. This exploratory study demonstrated that colloidal Ag NPs-based SERS serum analysis technique combined with PCA-LDA has a great potential in improving the detection of T. spiralis infection and onsite screening. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/31866336/Label-free_serum_detection_of_Trichinella_spiralis_using_surface-enhanced_Raman_spectroscopy_combined_with_multivariate_analysis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(19)31456-1 DB - PRIME DP - Unbound Medicine ER -