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[Effect of electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) on EIF2α-ATF4-GRP78/Bip signaling in substantia nigra of rats with Parkinson's disease].
Zhen Ci Yan Jiu. 2019 Dec 25; 44(12):873-7.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on expression of eukaryotic initiation factor 2α (EIF2α), activating transcription factor 4 (ATF4), glucose regulator protein-78 / immunoglobulin heavy-chain-binding protein (GRP78/Bip) in the substantia nigra (SN) in rats with Parkinson's disease (PD), so as to explore its mechanism underlying improvement of PD.

METHODS

Forty-eight male SD rats were randomly divided into control, sham-operation, model and EA groups (n=12 in each group). The PD model was established by 28-day consecutive subcutaneous injection of rotenone (1 mg/kg dissolved in dimethyl sulfoxide and normal saline) at the back shoulder. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV16) and "Taichong" (LR3) for 30 min, once daily for 2 weeks. The behavio-ral changes of rats in each group were measured and scored at 28th day and 44th day, respectively. The expressions of tyrosine hydroxylase (TH) and α-synuclein (α-syn) in the SN were observed by immunohistochemistry, and the expressions of EIF2α, ATF4 and GRP78/Bip were detected by Western blot.

RESULTS

Following modeling and compared with the control and sham-operation groups, the behavioral scores of rats in the model group were elevated (P<0.01), which were significantly decreased by EA intervention (P<0.01). The expression of TH decreased whereas the α-syn, EIF2α, ATF4 and GRP78/Bip increased in the rats of model group, and EA intervention reversed these changes (all P<0.01).

CONCLUSION

EA at GV16 and LR3 can improve PD rats' behavioral changes, which is probably related with its effects in up-regulating the expression of TH in the SN and down-regulating the expression of α-syn and EIF2α-ATF4-GRP78/Bip signaling.

Authors+Show Affiliations

College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.College of Acupuncture-moxibustion and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion, Wuhan 430060, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31867905

Citation

Ma, Jun, et al. "[Effect of Electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) On EIF2α-ATF4-GRP78/Bip Signaling in Substantia Nigra of Rats With Parkinson's Disease]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 12, 2019, pp. 873-7.
Ma J, Wang Y, Wang SJ, et al. [Effect of electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) on EIF2α-ATF4-GRP78/Bip signaling in substantia nigra of rats with Parkinson's disease]. Zhen Ci Yan Jiu. 2019;44(12):873-7.
Ma, J., Wang, Y., Wang, S. J., Yuan, L., Li, Y. N., & Yu, B. L. (2019). [Effect of electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) on EIF2α-ATF4-GRP78/Bip signaling in substantia nigra of rats with Parkinson's disease]. Zhen Ci Yan Jiu = Acupuncture Research, 44(12), 873-7. https://doi.org/10.13702/j.1000-0607.190193
Ma J, et al. [Effect of Electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) On EIF2α-ATF4-GRP78/Bip Signaling in Substantia Nigra of Rats With Parkinson's Disease]. Zhen Ci Yan Jiu. 2019 Dec 25;44(12):873-7. PubMed PMID: 31867905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture of "Fengfu" (GV16) and "Taichong" (LR3) on EIF2α-ATF4-GRP78/Bip signaling in substantia nigra of rats with Parkinson's disease]. AU - Ma,Jun, AU - Wang,Yao, AU - Wang,Shu-Ju, AU - Yuan,Li, AU - Li,Ya-Nan, AU - Yu,Ben-Lu, PY - 2019/12/24/entrez PY - 2019/12/24/pubmed PY - 2020/1/3/medline KW - Activating transcription factor 4 KW - Eukaryotic initiation factor 2α KW - Glucose-regu-lated protein 78 KW - Parkinson's disease SP - 873 EP - 7 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 12 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on expression of eukaryotic initiation factor 2α (EIF2α), activating transcription factor 4 (ATF4), glucose regulator protein-78 / immunoglobulin heavy-chain-binding protein (GRP78/Bip) in the substantia nigra (SN) in rats with Parkinson's disease (PD), so as to explore its mechanism underlying improvement of PD. METHODS: Forty-eight male SD rats were randomly divided into control, sham-operation, model and EA groups (n=12 in each group). The PD model was established by 28-day consecutive subcutaneous injection of rotenone (1 mg/kg dissolved in dimethyl sulfoxide and normal saline) at the back shoulder. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV16) and "Taichong" (LR3) for 30 min, once daily for 2 weeks. The behavio-ral changes of rats in each group were measured and scored at 28th day and 44th day, respectively. The expressions of tyrosine hydroxylase (TH) and α-synuclein (α-syn) in the SN were observed by immunohistochemistry, and the expressions of EIF2α, ATF4 and GRP78/Bip were detected by Western blot. RESULTS: Following modeling and compared with the control and sham-operation groups, the behavioral scores of rats in the model group were elevated (P<0.01), which were significantly decreased by EA intervention (P<0.01). The expression of TH decreased whereas the α-syn, EIF2α, ATF4 and GRP78/Bip increased in the rats of model group, and EA intervention reversed these changes (all P<0.01). CONCLUSION: EA at GV16 and LR3 can improve PD rats' behavioral changes, which is probably related with its effects in up-regulating the expression of TH in the SN and down-regulating the expression of α-syn and EIF2α-ATF4-GRP78/Bip signaling. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31867905/[Effect_of_electroacupuncture_of_"Fengfu"__GV16__and_"Taichong"__LR3__on_EIF2α_ATF4_GRP78/Bip_signaling_in_substantia_nigra_of_rats_with_Parkinson's_disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -