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[Transcriptome analysis of venom gland and identification of functional genes for snake venom protein in Agkistrodon acutus].
Zhongguo Zhong Yao Za Zhi. 2019 Nov; 44(22):4820-4829.ZZ

Abstract

Agkistrodon acutus is a traditional Chinese herb medicine which has immunological regulation,anti-tumor,anti-inflammatory and analgesic effects,which is mainly used for the treatment of rheumatoid arthritis,ankylosing spondylitis,sjogren's syndrome and tumors. In order to excavate more important functional genes from A. acutus,the transcriptome of the venom gland was sequenced by the Illumina Hi Seq 4000,and 32 862 unigenes were assembled. Among them,26 589 unigenes were mapped to least one public database. 2 695 unigenes were annotated and assigned to 62 TF families,and 5 920 SSR loci were identified. The majority of mapped unigenes was from Protobothrops mucrosquamatus in the NR database,which revealed their closest homology. Three secretory phospholipase A_2 with different amino acid sequences showed similar spatial structures and all had well-conserved active sites. The 3 D structural models of C-type lectin showed conserved glycosylation binding sites(Asn45). This study will lay the foundation for the further study of the function of snake venom protein,and promoting the development and utilization of genome resources from A. acutus.

Authors+Show Affiliations

Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China.Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China.Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China.Anhui Institute of Qi'men Snake Medicine Huangshang 245600,China.Anhui Institute of Qi'men Snake Medicine Huangshang 245600,China.Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Anhui Institute of Qi'men Snake Medicine Huangshang 245600,China.Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement Hefei 230012,China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31872588

Citation

Zhang, Sheng-Xiang, et al. "[Transcriptome Analysis of Venom Gland and Identification of Functional Genes for Snake Venom Protein in Agkistrodon Acutus]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 44, no. 22, 2019, pp. 4820-4829.
Zhang SX, Shi YY, Shan CM, et al. [Transcriptome analysis of venom gland and identification of functional genes for snake venom protein in Agkistrodon acutus]. Zhongguo Zhong Yao Za Zhi. 2019;44(22):4820-4829.
Zhang, S. X., Shi, Y. Y., Shan, C. M., Wang, T., Wang, Z. X., Wang, S. S., & Wu, J. W. (2019). [Transcriptome analysis of venom gland and identification of functional genes for snake venom protein in Agkistrodon acutus]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 44(22), 4820-4829. https://doi.org/10.19540/j.cnki.cjcmm.20190829.105
Zhang SX, et al. [Transcriptome Analysis of Venom Gland and Identification of Functional Genes for Snake Venom Protein in Agkistrodon Acutus]. Zhongguo Zhong Yao Za Zhi. 2019;44(22):4820-4829. PubMed PMID: 31872588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Transcriptome analysis of venom gland and identification of functional genes for snake venom protein in Agkistrodon acutus]. AU - Zhang,Sheng-Xiang, AU - Shi,Yuan-Yuan, AU - Shan,Chun-Miao, AU - Wang,Tao, AU - Wang,Zhen-Xing, AU - Wang,Sheng-Song, AU - Wu,Jia-Wen, PY - 2019/12/25/entrez PY - 2019/12/25/pubmed PY - 2020/1/11/medline KW - Agkistrodon acutus KW - C-type lectin KW - secretory phospholipase A_2 KW - transcriptome KW - venom gland SP - 4820 EP - 4829 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 44 IS - 22 N2 - Agkistrodon acutus is a traditional Chinese herb medicine which has immunological regulation,anti-tumor,anti-inflammatory and analgesic effects,which is mainly used for the treatment of rheumatoid arthritis,ankylosing spondylitis,sjogren's syndrome and tumors. In order to excavate more important functional genes from A. acutus,the transcriptome of the venom gland was sequenced by the Illumina Hi Seq 4000,and 32 862 unigenes were assembled. Among them,26 589 unigenes were mapped to least one public database. 2 695 unigenes were annotated and assigned to 62 TF families,and 5 920 SSR loci were identified. The majority of mapped unigenes was from Protobothrops mucrosquamatus in the NR database,which revealed their closest homology. Three secretory phospholipase A_2 with different amino acid sequences showed similar spatial structures and all had well-conserved active sites. The 3 D structural models of C-type lectin showed conserved glycosylation binding sites(Asn45). This study will lay the foundation for the further study of the function of snake venom protein,and promoting the development and utilization of genome resources from A. acutus. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/31872588/[Transcriptome_analysis_of_venom_gland_and_identification_of_functional_genes_for_snake_venom_protein_in_Agkistrodon_acutus] DB - PRIME DP - Unbound Medicine ER -