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[Effects of MT on growth and gonad development of natural overwintering Whitmania pigra].
Zhongguo Zhong Yao Za Zhi. 2019 Oct; 44(20):4426-4432.ZZ

Abstract

The objectives of study were to explore the effects of exogenous methyltestosterone(MT) on the growth and gonadal development of overwintering Whitmania pigra. Before overwintering,0. 1,1. 0,10. 0,100. 0,150. 0 μg·L-1 of MT were added to the aquaculture water for 6 weeks. The changes of growth performance,gonad index,endogenous steroid hormones level and internal quality were measured after hibernate for 60 days. Then the tissue slice technique was used to observe the spermary(ovary) of Wh. pigra.The results showed that the body weight,survival rate and gonadal index increased first and then decreased with the increase of exogenous MT concentration; the male gland index was found the highest at the concentration of MT 10. 0 μg·L-1 and the female gland index was the highest at the concentration of MT 1. 0 μg·L-1. The survival rate of Wh. pigra peaked at the concentration of MT 10. 0 μg·L-1.The weight reaches a peak at a concentration of MT 100. 0 μg·L-1(P<0. 05). The number of primary spermatocytes in the testis was negatively correlated with the concentration of exogenous MT. The number of secondary spermatocytes and sperm cells increased first and then decreased. The concentration of secondary spermatocytes was the highest when the concentration of MT was 100. 0 μg·L-1.The number and volume of oocytes in the ovary and the yolk granules increased first and then decreased with the increase of exogenous MT concentration,and the highest was observed at the MT concentration of 100. 0 μg·L-1. The endogenous steroid hormone of Wh.pigra increased first and then decreased with the increase of exogenous MT concentration. The concentration of androgen and progesterone was the highest in MT 100. 0 μg·L-1 treatment(P<0. 05),and the concentration of estrogen was found the highest in MT 10. 0 μg·L-1 treatment(P<0. 05). After adding exogenous MT,Wh. pigra moisture content,acid-insoluble ash content,p H and anti-thrombin activity met the quality criterion of medicinal Wh. pigra in Chinese Pharmacopoeia(2015 edition). In conclusion,the short-term addition of 1. 0-100. 0 μg·L-1 exogenous MT before hibernation can promote the growth,the development of sperm cells and the antithrombin activity of Wh. pigra.

Authors+Show Affiliations

Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31872628

Citation

Lu, Xin, et al. "[Effects of MT On Growth and Gonad Development of Natural Overwintering Whitmania Pigra]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 44, no. 20, 2019, pp. 4426-4432.
Lu X, Guo QS, Shi HZ, et al. [Effects of MT on growth and gonad development of natural overwintering Whitmania pigra]. Zhongguo Zhong Yao Za Zhi. 2019;44(20):4426-4432.
Lu, X., Guo, Q. S., Shi, H. Z., Wang, S., Chen, P. P., Zhang, H. N., & Yan, X. L. (2019). [Effects of MT on growth and gonad development of natural overwintering Whitmania pigra]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 44(20), 4426-4432. https://doi.org/10.19540/j.cnki.cjcmm.20190729.104
Lu X, et al. [Effects of MT On Growth and Gonad Development of Natural Overwintering Whitmania Pigra]. Zhongguo Zhong Yao Za Zhi. 2019;44(20):4426-4432. PubMed PMID: 31872628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of MT on growth and gonad development of natural overwintering Whitmania pigra]. AU - Lu,Xin, AU - Guo,Qiao-Sheng, AU - Shi,Hong-Zhuan, AU - Wang,Shuai, AU - Chen,Pan-Pan, AU - Zhang,Hui-Ning, AU - Yan,Xiao-Lu, PY - 2019/12/25/entrez PY - 2019/12/25/pubmed PY - 2020/1/16/medline KW - Whitmania pigra KW - gonadal development KW - growth KW - hormone KW - methyltestosterone SP - 4426 EP - 4432 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 44 IS - 20 N2 - The objectives of study were to explore the effects of exogenous methyltestosterone(MT) on the growth and gonadal development of overwintering Whitmania pigra. Before overwintering,0. 1,1. 0,10. 0,100. 0,150. 0 μg·L-1 of MT were added to the aquaculture water for 6 weeks. The changes of growth performance,gonad index,endogenous steroid hormones level and internal quality were measured after hibernate for 60 days. Then the tissue slice technique was used to observe the spermary(ovary) of Wh. pigra.The results showed that the body weight,survival rate and gonadal index increased first and then decreased with the increase of exogenous MT concentration; the male gland index was found the highest at the concentration of MT 10. 0 μg·L-1 and the female gland index was the highest at the concentration of MT 1. 0 μg·L-1. The survival rate of Wh. pigra peaked at the concentration of MT 10. 0 μg·L-1.The weight reaches a peak at a concentration of MT 100. 0 μg·L-1(P<0. 05). The number of primary spermatocytes in the testis was negatively correlated with the concentration of exogenous MT. The number of secondary spermatocytes and sperm cells increased first and then decreased. The concentration of secondary spermatocytes was the highest when the concentration of MT was 100. 0 μg·L-1.The number and volume of oocytes in the ovary and the yolk granules increased first and then decreased with the increase of exogenous MT concentration,and the highest was observed at the MT concentration of 100. 0 μg·L-1. The endogenous steroid hormone of Wh.pigra increased first and then decreased with the increase of exogenous MT concentration. The concentration of androgen and progesterone was the highest in MT 100. 0 μg·L-1 treatment(P<0. 05),and the concentration of estrogen was found the highest in MT 10. 0 μg·L-1 treatment(P<0. 05). After adding exogenous MT,Wh. pigra moisture content,acid-insoluble ash content,p H and anti-thrombin activity met the quality criterion of medicinal Wh. pigra in Chinese Pharmacopoeia(2015 edition). In conclusion,the short-term addition of 1. 0-100. 0 μg·L-1 exogenous MT before hibernation can promote the growth,the development of sperm cells and the antithrombin activity of Wh. pigra. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/31872628/[Effects_of_MT_on_growth_and_gonad_development_of_natural_overwintering_Whitmania_pigra] DB - PRIME DP - Unbound Medicine ER -