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Duhuo jisheng decoction suppresses matrix degradation and apoptosis in human nucleus pulposus cells and ameliorates disc degeneration in a rat model.
J Ethnopharmacol. 2020 Mar 25; 250:112494.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The lower back pain (LBP) caused by intervertebral disc (IVD) degeneration brings a heavy burden to society. A classic treatment method of Chinese medicine, fangji-duhuo jisheng decoction (DHJSD), has been effective in the clinical treatment of LBP, although the underlying mechanism remains unknown.

AIM OF THE STUDY

In this work, the main objective was to study the effects of DHJSD on in vitro IVD degeneration of human nucleus pulposus (NP) cells after pressure treatment and on an in vivo interrupted IVD degeneration rat model.

MATERIALS AND METHODS

The effects of DHJSD on the viability of NP cells were detected using Cell Counting Kit-8. RT-qPCR, western blotting, TUNEL assay, transmission electron microscopy, and immunofluorescence staining were performed to explore the molecular mechanism underlying protection against compression-induced matrix degradation and apoptosis in NP cells by DHJSD. Furthermore, the effects of DHJSD on IVD degeneration in a rat IDD model were also determined.

RESULTS

We found that DHJSD increased the viability of NP cells in a concentration- and time-dependent manner. Furthermore, DHJSD significantly reduced compression-induced NP matrix degeneration and apoptosis, activated autophagy, and inhibited the p38/MAPK signaling pathway in NP cells subjected to compression. Autophagy inhibitor 3-MA and p38/MAPK signaling pathway activator anisomycin reversed the beneficial effects of DHJSD in NP cells, indicating that DHJSD protects against IVD degeneration by autophagy activation and P38/MAPK signaling pathway inhibition. Furthermore, DHJSD treatment effectively delayed IVD degeneration in a puncture-induced IDD rat model.

CONCLUSIONS

DHJSD prevents compression-induced matrix degradation and cell apoptosis through regulating autophagy and the P38/MAPK signaling pathway. The mechanism underlying the effects of DHSJD elucidated in this study provides a new direction for LBP treatment.

Authors+Show Affiliations

Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China.College of Acupuncture of Orthopedics, Hubei University of Chinese Medicine, Wuhan, 430061, China.Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China.College of Acupuncture of Orthopedics, Hubei University of Chinese Medicine, Wuhan, 430061, China.Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China.College of Acupuncture of Orthopedics, Hubei University of Chinese Medicine, Wuhan, 430061, China.Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China.Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China. Electronic address: xiapingfm@126.com.Department of Orthopaedics, First Hospital of Wuhan, Wuhan, 430022, China. Electronic address: whsdyyyjzwk@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31874213

Citation

Liu, Wei, et al. "Duhuo Jisheng Decoction Suppresses Matrix Degradation and Apoptosis in Human Nucleus Pulposus Cells and Ameliorates Disc Degeneration in a Rat Model." Journal of Ethnopharmacology, vol. 250, 2020, p. 112494.
Liu W, Jin S, Huang M, et al. Duhuo jisheng decoction suppresses matrix degradation and apoptosis in human nucleus pulposus cells and ameliorates disc degeneration in a rat model. J Ethnopharmacol. 2020;250:112494.
Liu, W., Jin, S., Huang, M., Li, Y., Wang, Z., Wang, P., Zhao, X., Xia, P., & Feng, J. (2020). Duhuo jisheng decoction suppresses matrix degradation and apoptosis in human nucleus pulposus cells and ameliorates disc degeneration in a rat model. Journal of Ethnopharmacology, 250, 112494. https://doi.org/10.1016/j.jep.2019.112494
Liu W, et al. Duhuo Jisheng Decoction Suppresses Matrix Degradation and Apoptosis in Human Nucleus Pulposus Cells and Ameliorates Disc Degeneration in a Rat Model. J Ethnopharmacol. 2020 Mar 25;250:112494. PubMed PMID: 31874213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Duhuo jisheng decoction suppresses matrix degradation and apoptosis in human nucleus pulposus cells and ameliorates disc degeneration in a rat model. AU - Liu,Wei, AU - Jin,Shuwen, AU - Huang,Mi, AU - Li,Yanli, AU - Wang,ZhiWei, AU - Wang,Peng, AU - Zhao,Xiaolong, AU - Xia,Ping, AU - Feng,Jing, Y1 - 2019/12/24/ PY - 2019/10/20/received PY - 2019/12/05/revised PY - 2019/12/19/accepted PY - 2019/12/25/pubmed PY - 2019/12/25/medline PY - 2019/12/25/entrez KW - Autophagy KW - Duhuo jisheng decoction KW - Intervertebral disc degeneration KW - Nucleus pulposus KW - p38 SP - 112494 EP - 112494 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 250 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The lower back pain (LBP) caused by intervertebral disc (IVD) degeneration brings a heavy burden to society. A classic treatment method of Chinese medicine, fangji-duhuo jisheng decoction (DHJSD), has been effective in the clinical treatment of LBP, although the underlying mechanism remains unknown. AIM OF THE STUDY: In this work, the main objective was to study the effects of DHJSD on in vitro IVD degeneration of human nucleus pulposus (NP) cells after pressure treatment and on an in vivo interrupted IVD degeneration rat model. MATERIALS AND METHODS: The effects of DHJSD on the viability of NP cells were detected using Cell Counting Kit-8. RT-qPCR, western blotting, TUNEL assay, transmission electron microscopy, and immunofluorescence staining were performed to explore the molecular mechanism underlying protection against compression-induced matrix degradation and apoptosis in NP cells by DHJSD. Furthermore, the effects of DHJSD on IVD degeneration in a rat IDD model were also determined. RESULTS: We found that DHJSD increased the viability of NP cells in a concentration- and time-dependent manner. Furthermore, DHJSD significantly reduced compression-induced NP matrix degeneration and apoptosis, activated autophagy, and inhibited the p38/MAPK signaling pathway in NP cells subjected to compression. Autophagy inhibitor 3-MA and p38/MAPK signaling pathway activator anisomycin reversed the beneficial effects of DHJSD in NP cells, indicating that DHJSD protects against IVD degeneration by autophagy activation and P38/MAPK signaling pathway inhibition. Furthermore, DHJSD treatment effectively delayed IVD degeneration in a puncture-induced IDD rat model. CONCLUSIONS: DHJSD prevents compression-induced matrix degradation and cell apoptosis through regulating autophagy and the P38/MAPK signaling pathway. The mechanism underlying the effects of DHSJD elucidated in this study provides a new direction for LBP treatment. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/31874213/Duhuo_jisheng_decoction_suppresses_matrix_degradation_and_apoptosis_in_human_nucleus_pulposus_cells_and_ameliorates_disc_degeneration_in_a_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(19)34193-5 DB - PRIME DP - Unbound Medicine ER -
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