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Duodenal Infusions of Starch with Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle.
J Nutr. 2020 04 01; 150(4):784-791.JN

Abstract

BACKGROUND

Small intestinal starch digestion in ruminants is potentially limited by inadequate production of carbohydrases. Previous research has demonstrated that small intestinal starch digestion can be improved by postruminal supply of casein or glutamic acid. However, the mechanisms by which casein and glutamic acid increase starch digestion are not well understood.

OBJECTIVES

The objective of this experiment was to evaluate the effects of duodenal infusions of starch with casein or glutamic acid on postruminal carbohydrase activities in cattle.

METHODS

Twenty-two steers [mean body weight (BW) = 179 ± 4.23 kg] were surgically fitted with duodenal and ileal cannulas and limit-fed a soybean hull-based diet containing small amounts of starch. Raw cornstarch (1.61 ± 0.0869 kg/d) was infused into the duodenum alone (control), or with 118 ± 7.21 g glutamic acid/d, or 428 ± 19.4 g casein/d. Treatments were infused continuously for 58 d and then steers were killed for tissue collection. Activities of pancreatic (α-amylase) and intestinal (maltase, isomaltase, glucoamylase, sucrase) carbohydrases were determined. Data were analyzed as a randomized complete block (replicate group) design using the GLM procedure of SAS to determine effects of infusion treatment.

RESULTS

Duodenal casein infusion increased (P < 0.05) pancreatic α-amylase activity by 290%. Duodenal glutamic acid infusion increased (P < 0.03) duodenal maltase activity by 233%. Duodenal casein infusion increased jejunal maltase (P = 0.02) and glucoamylase (P = 0.03) activity per gram protein by 62.9% and 97.4%, respectively. Duodenal casein infusion tended to increase (P = 0.10) isomaltase activity per gram jejunum by 38.5% in the jejunum. Sucrase activity was not detected in any segment of the small intestine.

CONCLUSIONS

These results suggest that small intestinal starch digestion can be improved in cattle with increased small intestinal flow of casein through increases in postruminal carbohydrase activities.

Authors+Show Affiliations

Department of Animal Sciences, North Dakota State University, Fargo, ND, USA.Department of Animal Sciences, North Dakota State University, Fargo, ND, USA.Department of Animal Science, South Dakota State University, Brookings, SD, USA.Department of Animal Science, South Dakota State University, Brookings, SD, USA.Department of Animal Sciences, North Dakota State University, Fargo, ND, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31875476

Citation

Trotta, Ronald J., et al. "Duodenal Infusions of Starch With Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle." The Journal of Nutrition, vol. 150, no. 4, 2020, pp. 784-791.
Trotta RJ, Sitorski LG, Acharya S, et al. Duodenal Infusions of Starch with Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle. J Nutr. 2020;150(4):784-791.
Trotta, R. J., Sitorski, L. G., Acharya, S., Brake, D. W., & Swanson, K. C. (2020). Duodenal Infusions of Starch with Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle. The Journal of Nutrition, 150(4), 784-791. https://doi.org/10.1093/jn/nxz319
Trotta RJ, et al. Duodenal Infusions of Starch With Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle. J Nutr. 2020 04 1;150(4):784-791. PubMed PMID: 31875476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Duodenal Infusions of Starch with Casein or Glutamic Acid Influence Pancreatic and Small Intestinal Carbohydrase Activities in Cattle. AU - Trotta,Ronald J, AU - Sitorski,Leonardo G, AU - Acharya,Subash, AU - Brake,Derek W, AU - Swanson,Kendall C, PY - 2019/08/19/received PY - 2019/10/10/revised PY - 2019/12/03/accepted PY - 2019/12/26/pubmed PY - 2019/12/26/medline PY - 2019/12/26/entrez KW - cattle KW - disaccharidase KW - glucoamylase KW - isomaltase KW - maltase KW - postruminal digestion KW - ruminant KW - starch digestion KW - sucrase KW - α-amylase SP - 784 EP - 791 JF - The Journal of nutrition JO - J. Nutr. VL - 150 IS - 4 N2 - BACKGROUND: Small intestinal starch digestion in ruminants is potentially limited by inadequate production of carbohydrases. Previous research has demonstrated that small intestinal starch digestion can be improved by postruminal supply of casein or glutamic acid. However, the mechanisms by which casein and glutamic acid increase starch digestion are not well understood. OBJECTIVES: The objective of this experiment was to evaluate the effects of duodenal infusions of starch with casein or glutamic acid on postruminal carbohydrase activities in cattle. METHODS: Twenty-two steers [mean body weight (BW) = 179 ± 4.23 kg] were surgically fitted with duodenal and ileal cannulas and limit-fed a soybean hull-based diet containing small amounts of starch. Raw cornstarch (1.61 ± 0.0869 kg/d) was infused into the duodenum alone (control), or with 118 ± 7.21 g glutamic acid/d, or 428 ± 19.4 g casein/d. Treatments were infused continuously for 58 d and then steers were killed for tissue collection. Activities of pancreatic (α-amylase) and intestinal (maltase, isomaltase, glucoamylase, sucrase) carbohydrases were determined. Data were analyzed as a randomized complete block (replicate group) design using the GLM procedure of SAS to determine effects of infusion treatment. RESULTS: Duodenal casein infusion increased (P < 0.05) pancreatic α-amylase activity by 290%. Duodenal glutamic acid infusion increased (P < 0.03) duodenal maltase activity by 233%. Duodenal casein infusion increased jejunal maltase (P = 0.02) and glucoamylase (P = 0.03) activity per gram protein by 62.9% and 97.4%, respectively. Duodenal casein infusion tended to increase (P = 0.10) isomaltase activity per gram jejunum by 38.5% in the jejunum. Sucrase activity was not detected in any segment of the small intestine. CONCLUSIONS: These results suggest that small intestinal starch digestion can be improved in cattle with increased small intestinal flow of casein through increases in postruminal carbohydrase activities. SN - 1541-6100 UR - https://www.unboundmedicine.com/medline/citation/31875476/Duodenal_Infusions_of_Starch_with_Casein_or_Glutamic_Acid_Influence_Pancreatic_and_Small_Intestinal_Carbohydrase_Activities_in_Cattle L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/nxz319 DB - PRIME DP - Unbound Medicine ER -
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