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Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts from Asclepias linaria Cav. Leaves.
Molecules. 2019 Dec 23; 25(1)M

Abstract

Asclepias linaria Cav. (Apocynaceae) is a shrubby plant endemic of Mexico which has been used in traditional medicine. However, the bioactive potential of this plant remains unexplored. In this study, the phenolic composition, antioxidant, and cytotoxic activities of A. linaria leaves were determined. In order to estimate the phenolic composition of the leaves, the total phenolic, flavonoid, and condensed tannins contents were determined. Furthermore, the antioxidant activity was measured by the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS•+) radicals and the total antioxidant capacity. The phenolic compounds identified in the A. linaria leaves by ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) include phenolic acids, such as p-coumaric and ferulic acid, as well as flavonoids, such as rutin and quercetin. The leaves' extracts of A. linaria showed a high scavenging activity of DPPH• and ABTS•+ radicals (IC50 0.12 ± 0.001 and 0.51 ± 0.003 µg/mL, respectively), high total antioxidant capacity values (99.77 ± 4.32 mg of ascorbic acid equivalents/g of dry tissue), and had a cytotoxic effect against K562 and HL60 hematologic neoplasia cells lines, but no toxicity towards the normal mononuclear cell line was observed. These results highlight the potential of A. linaria and could be considered as a possible alternative source of anticancer compounds.

Authors+Show Affiliations

Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Jiquilpan 59510, Michoacán, Mexico.CONACYT-Instituto Nacional de Pediatría, Coyoacán 04530, Ciudad de México, Mexico.Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Jiquilpan 59510, Michoacán, Mexico.Unidad de Servicios Analíticos y Metrológicos, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Guadalajara 44270, Jalisco, Mexico.CONACYT - Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Jiquilpan 59510, Michoacán, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31877920

Citation

Sánchez-Gutiérrez, José Alejandro, et al. "Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts From Asclepias Linaria Cav. Leaves." Molecules (Basel, Switzerland), vol. 25, no. 1, 2019.
Sánchez-Gutiérrez JA, Moreno-Lorenzana D, Álvarez-Bernal D, et al. Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts from Asclepias linaria Cav. Leaves. Molecules. 2019;25(1).
Sánchez-Gutiérrez, J. A., Moreno-Lorenzana, D., Álvarez-Bernal, D., Rodríguez-Campos, J., & Medina-Medrano, J. R. (2019). Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts from Asclepias linaria Cav. Leaves. Molecules (Basel, Switzerland), 25(1). https://doi.org/10.3390/molecules25010054
Sánchez-Gutiérrez JA, et al. Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts From Asclepias Linaria Cav. Leaves. Molecules. 2019 Dec 23;25(1) PubMed PMID: 31877920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts from Asclepias linaria Cav. Leaves. AU - Sánchez-Gutiérrez,José Alejandro, AU - Moreno-Lorenzana,Dafné, AU - Álvarez-Bernal,Dioselina, AU - Rodríguez-Campos,Jacobo, AU - Medina-Medrano,José Roberto, Y1 - 2019/12/23/ PY - 2019/11/20/received PY - 2019/12/17/revised PY - 2019/12/18/accepted PY - 2019/12/28/entrez PY - 2019/12/28/pubmed PY - 2020/5/30/medline KW - Asclepias linaria KW - antioxidant activity KW - cytotoxic activity KW - phenolic compounds KW - scavenging activity KW - ultra-performance liquid chromatography (UPLC) JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 1 N2 - Asclepias linaria Cav. (Apocynaceae) is a shrubby plant endemic of Mexico which has been used in traditional medicine. However, the bioactive potential of this plant remains unexplored. In this study, the phenolic composition, antioxidant, and cytotoxic activities of A. linaria leaves were determined. In order to estimate the phenolic composition of the leaves, the total phenolic, flavonoid, and condensed tannins contents were determined. Furthermore, the antioxidant activity was measured by the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS•+) radicals and the total antioxidant capacity. The phenolic compounds identified in the A. linaria leaves by ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) include phenolic acids, such as p-coumaric and ferulic acid, as well as flavonoids, such as rutin and quercetin. The leaves' extracts of A. linaria showed a high scavenging activity of DPPH• and ABTS•+ radicals (IC50 0.12 ± 0.001 and 0.51 ± 0.003 µg/mL, respectively), high total antioxidant capacity values (99.77 ± 4.32 mg of ascorbic acid equivalents/g of dry tissue), and had a cytotoxic effect against K562 and HL60 hematologic neoplasia cells lines, but no toxicity towards the normal mononuclear cell line was observed. These results highlight the potential of A. linaria and could be considered as a possible alternative source of anticancer compounds. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31877920/Phenolic_Profile_Antioxidant_and_Anti_Proliferative_Activities_of_Methanolic_Extracts_from_Asclepias_linaria_Cav__Leaves_ L2 - https://www.mdpi.com/resolver?pii=molecules25010054 DB - PRIME DP - Unbound Medicine ER -