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Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse.
Bioresour Technol. 2020 Mar; 299:122625.BT

Abstract

The production of high-purity xylobiose from lignocellulose is an expensive and tedious process. In this work, the production of xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse was investigated. Furthermore, a simple process for the separation of xylobiose from enzymatic hydrolysate by activated carbon absorption, water washing, and ethanol-water desorption was developed. Under the optimized separation conditions, 96.77% xylobiose was adsorbed at 16% activated carbon loadings. Moreover, xylose and acetate could not be detected after washing by 3-fold volume of water. Xylobiose with 80.16% yield was eluted by 5-fold volume of 5% (v/v) ethanol-water. The reusability of activated carbon was evaluated by 5 cycles of adsorption-desorption process, suggesting that the activated carbon exhibited good reusability. The separated xylobiose sample with high-purity (97.29%) was confirmed by HPLC, ESI-MS, and NMR. Overall, this study provided a low-cost and robust technology for the production and separation of high-purity xylobiose from lignocellulose.

Authors+Show Affiliations

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, People's Republic of China. Electronic address: cxd_cxd@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31881437

Citation

Li, Hailong, et al. "Production, Separation, and Characterization of High-purity Xylobiose From Enzymatic Hydrolysis of Alkaline Oxidation Pretreated Sugarcane Bagasse." Bioresource Technology, vol. 299, 2020, p. 122625.
Li H, Chen X, Xiong L, et al. Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse. Bioresour Technol. 2020;299:122625.
Li, H., Chen, X., Xiong, L., Zhang, L., Chen, X., Wang, C., Huang, C., & Chen, X. (2020). Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse. Bioresource Technology, 299, 122625. https://doi.org/10.1016/j.biortech.2019.122625
Li H, et al. Production, Separation, and Characterization of High-purity Xylobiose From Enzymatic Hydrolysis of Alkaline Oxidation Pretreated Sugarcane Bagasse. Bioresour Technol. 2020;299:122625. PubMed PMID: 31881437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse. AU - Li,Hailong, AU - Chen,Xindong, AU - Xiong,Lian, AU - Zhang,Liquan, AU - Chen,Xuefang, AU - Wang,Can, AU - Huang,Chao, AU - Chen,Xinde, Y1 - 2019/12/14/ PY - 2019/10/21/received PY - 2019/12/11/revised PY - 2019/12/12/accepted PY - 2019/12/28/pubmed PY - 2020/1/28/medline PY - 2019/12/28/entrez KW - Activated carbon adsorption KW - Ethanol-water desorption KW - High-purity xylobiose KW - Separation SP - 122625 EP - 122625 JF - Bioresource technology JO - Bioresour. Technol. VL - 299 N2 - The production of high-purity xylobiose from lignocellulose is an expensive and tedious process. In this work, the production of xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse was investigated. Furthermore, a simple process for the separation of xylobiose from enzymatic hydrolysate by activated carbon absorption, water washing, and ethanol-water desorption was developed. Under the optimized separation conditions, 96.77% xylobiose was adsorbed at 16% activated carbon loadings. Moreover, xylose and acetate could not be detected after washing by 3-fold volume of water. Xylobiose with 80.16% yield was eluted by 5-fold volume of 5% (v/v) ethanol-water. The reusability of activated carbon was evaluated by 5 cycles of adsorption-desorption process, suggesting that the activated carbon exhibited good reusability. The separated xylobiose sample with high-purity (97.29%) was confirmed by HPLC, ESI-MS, and NMR. Overall, this study provided a low-cost and robust technology for the production and separation of high-purity xylobiose from lignocellulose. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/31881437/Production,_separation,_and_characterization_of_high-purity_xylobiose_from_enzymatic_hydrolysis_of_alkaline_oxidation_pretreated_sugarcane_bagasse L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(19)31855-3 DB - PRIME DP - Unbound Medicine ER -
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