Tags

Type your tag names separated by a space and hit enter

Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust.
BMC Plant Biol. 2019 Dec 27; 19(1):590.BP

Abstract

BACKGROUND

Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement.

RESULTS

Here, we report the development and characterization of two wheat-E. repens lines derived from the progeny of common wheat-E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15-1192-2 and K15-1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15-1192-2 was a wheat-E. repens 3D/?St double terminal chromosomal translocation line. Line K15-1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian translocations and one 3D/?St double terminal translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat-E. repens translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15-1192-2 and K15-1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China.

CONCLUSIONS

Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

Links

PMC Free PDF

Authors+Show Affiliations

Gong B
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhu W
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Li S
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xu L
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zeng J
College of Resources, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Fan X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Sha L
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qi P
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Huang L
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Chen G
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhou Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Kang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceElymusFusariumGenetic MarkersIn Situ Hybridization, FluorescenceMeiosisPlant BreedingPlant DiseasesTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31881925

Citation

Gong, Biran, et al. "Molecular Cytogenetic Characterization of wheat-Elymus Repens Chromosomal Translocation Lines With Resistance to Fusarium Head Blight and Stripe Rust." BMC Plant Biology, vol. 19, no. 1, 2019, p. 590.
Gong B, Zhu W, Li S, et al. Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust. BMC Plant Biol. 2019;19(1):590.
Gong, B., Zhu, W., Li, S., Wang, Y., Xu, L., Wang, Y., Zeng, J., Fan, X., Sha, L., Zhang, H., Qi, P., Huang, L., Chen, G., Zhou, Y., & Kang, H. (2019). Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust. BMC Plant Biology, 19(1), 590. https://doi.org/10.1186/s12870-019-2208-x
Gong B, et al. Molecular Cytogenetic Characterization of wheat-Elymus Repens Chromosomal Translocation Lines With Resistance to Fusarium Head Blight and Stripe Rust. BMC Plant Biol. 2019 Dec 27;19(1):590. PubMed PMID: 31881925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization of wheat-Elymus repens chromosomal translocation lines with resistance to Fusarium head blight and stripe rust. AU - Gong,Biran, AU - Zhu,Wei, AU - Li,Sanyue, AU - Wang,Yuqi, AU - Xu,Lili, AU - Wang,Yi, AU - Zeng,Jian, AU - Fan,Xing, AU - Sha,Lina, AU - Zhang,Haiqin, AU - Qi,Pengfei, AU - Huang,Lin, AU - Chen,Guoyue, AU - Zhou,Yonghong, AU - Kang,Houyang, Y1 - 2019/12/27/ PY - 2019/07/16/received PY - 2019/12/18/accepted PY - 2019/12/29/entrez PY - 2019/12/29/pubmed PY - 2020/3/13/medline KW - Chromosomal translocation line KW - Elymus repens KW - Fusarium head blight (FHB) KW - Stripe rust SP - 590 EP - 590 JF - BMC plant biology JO - BMC Plant Biol VL - 19 IS - 1 N2 - BACKGROUND: Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. RESULTS: Here, we report the development and characterization of two wheat-E. repens lines derived from the progeny of common wheat-E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15-1192-2 and K15-1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15-1192-2 was a wheat-E. repens 3D/?St double terminal chromosomal translocation line. Line K15-1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian translocations and one 3D/?St double terminal translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat-E. repens translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15-1192-2 and K15-1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. CONCLUSIONS: Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/31881925/Molecular_cytogenetic_characterization_of_wheat_Elymus_repens_chromosomal_translocation_lines_with_resistance_to_Fusarium_head_blight_and_stripe_rust_ DB - PRIME DP - Unbound Medicine ER -