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Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA.
Methods Mol Biol. 2020; 2099:127-133.MM

Abstract

Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1-725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies.

Authors+Show Affiliations

Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. amhashem@kau.edu.sa. Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. amhashem@kau.edu.sa.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31883093

Citation

Al-Amri, Sawsan S., and Anwar M. Hashem. "Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA." Methods in Molecular Biology (Clifton, N.J.), vol. 2099, 2020, pp. 127-133.
Al-Amri SS, Hashem AM. Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA. Methods Mol Biol. 2020;2099:127-133.
Al-Amri, S. S., & Hashem, A. M. (2020). Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA. Methods in Molecular Biology (Clifton, N.J.), 2099, 127-133. https://doi.org/10.1007/978-1-0716-0211-9_11
Al-Amri SS, Hashem AM. Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA. Methods Mol Biol. 2020;2099:127-133. PubMed PMID: 31883093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA. AU - Al-Amri,Sawsan S, AU - Hashem,Anwar M, PY - 2019/12/29/entrez PY - 2019/12/29/pubmed PY - 2020/9/22/medline KW - Antibodies KW - Antigens KW - ELISA KW - MERS-CoV KW - Recombinant S1 subunit KW - Serology SP - 127 EP - 133 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 2099 N2 - Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1-725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/31883093/Qualitative_and_Quantitative_Determination_of_MERS_CoV_S1_Specific_Antibodies_Using_ELISA_ L2 - https://dx.doi.org/10.1007/978-1-0716-0211-9_11 DB - PRIME DP - Unbound Medicine ER -