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Genotoxicity assessment of triclocarban by comet and micronucleus assays and Ames test.
Environ Sci Pollut Res Int. 2020 Mar; 27(7):7430-7438.ES

Abstract

The widespread usage and ubiquitous distribution of triclocarban (3,4,4'-trichlorocarbanilide, TCC) have raised public concerns about its health effects. At present, there is little information about the genotoxicity of TCC. In this study, we used a battery of genotoxicity testing methods including salmonella reverse mutation test (Ames test), comet assay and micronucleus assay to detect the effects of TCC on gene mutation, DNA breakage, and chromosome damage. The results of Ames test showed that TCC at 0.1-1000 μg/plate did not significantly increase the number of revertant colonies in the four standard Salmonella typhimurium strains, i.e., TA97, TA98, TA100, and TA102, when compared to the vehicle control. The results from comet assay demonstrated that exposure to 5, 10, or 15 μM TCC for 24 h did not significantly increase the percentage of comet cells, tail length (TL), DNA in tail (T DNA%), or olive tail moment (OTM) in keratinocyte HaCaT and hepatic L02 cells. Moreover, TCC did not markedly enhance the frequency of micronucleated cells or micronuclei in HaCaT and L02 cells in the micronucleus assay. Taken together, the results indicated that TCC did not exhibit any genotoxic effects. Our study provides additional information for the safety profile of TCC.

Authors+Show Affiliations

Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. zhangzunzhen@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31884548

Citation

Sun, Donglei, et al. "Genotoxicity Assessment of Triclocarban By Comet and Micronucleus Assays and Ames Test." Environmental Science and Pollution Research International, vol. 27, no. 7, 2020, pp. 7430-7438.
Sun D, Zhao T, Wang T, et al. Genotoxicity assessment of triclocarban by comet and micronucleus assays and Ames test. Environ Sci Pollut Res Int. 2020;27(7):7430-7438.
Sun, D., Zhao, T., Wang, T., Wu, M., & Zhang, Z. (2020). Genotoxicity assessment of triclocarban by comet and micronucleus assays and Ames test. Environmental Science and Pollution Research International, 27(7), 7430-7438. https://doi.org/10.1007/s11356-019-07351-9
Sun D, et al. Genotoxicity Assessment of Triclocarban By Comet and Micronucleus Assays and Ames Test. Environ Sci Pollut Res Int. 2020;27(7):7430-7438. PubMed PMID: 31884548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity assessment of triclocarban by comet and micronucleus assays and Ames test. AU - Sun,Donglei, AU - Zhao,Tianhe, AU - Wang,Ting, AU - Wu,Mei, AU - Zhang,Zunzhen, Y1 - 2019/12/28/ PY - 2019/06/19/received PY - 2019/12/09/accepted PY - 2019/12/31/pubmed PY - 2020/5/27/medline PY - 2019/12/30/entrez KW - Chromosome damage KW - DNA breakage KW - Genotoxicity KW - Mutagenicity KW - Risk assessment KW - Triclocarban SP - 7430 EP - 7438 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 27 IS - 7 N2 - The widespread usage and ubiquitous distribution of triclocarban (3,4,4'-trichlorocarbanilide, TCC) have raised public concerns about its health effects. At present, there is little information about the genotoxicity of TCC. In this study, we used a battery of genotoxicity testing methods including salmonella reverse mutation test (Ames test), comet assay and micronucleus assay to detect the effects of TCC on gene mutation, DNA breakage, and chromosome damage. The results of Ames test showed that TCC at 0.1-1000 μg/plate did not significantly increase the number of revertant colonies in the four standard Salmonella typhimurium strains, i.e., TA97, TA98, TA100, and TA102, when compared to the vehicle control. The results from comet assay demonstrated that exposure to 5, 10, or 15 μM TCC for 24 h did not significantly increase the percentage of comet cells, tail length (TL), DNA in tail (T DNA%), or olive tail moment (OTM) in keratinocyte HaCaT and hepatic L02 cells. Moreover, TCC did not markedly enhance the frequency of micronucleated cells or micronuclei in HaCaT and L02 cells in the micronucleus assay. Taken together, the results indicated that TCC did not exhibit any genotoxic effects. Our study provides additional information for the safety profile of TCC. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/31884548/Genotoxicity_assessment_of_triclocarban_by_comet_and_micronucleus_assays_and_Ames_test L2 - https://dx.doi.org/10.1007/s11356-019-07351-9 DB - PRIME DP - Unbound Medicine ER -
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