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Hydrogen sulfide modulates high glucose-induced NLRP3 inflammasome activation in 3T3-L1 adipocytes.
Exp Ther Med 2020; 19(1):771-776ET

Abstract

Activation of the NACHT leucine rich repeat and pyd domains-containing 3 (NLRP3) inflammasome plays an important role in the initiation of inflammation in adipose tissue in diabetic patients. However, the mechanisms underlying this are not fully understood. Hydrogen sulfide (H2S) has been shown to have anti-inflammatory properties in various cell types. The present study aimed to investigate the effect of H2S on high glucose (HG)-induced NLRP3 inflammasome activation in adipocytes. Adipocytes were differentiated from 3T3-L1 cells and treated with low glucose (LG), HG, H2S donor sodium hydrosulfide (NaHS) or N-acetyl-tyrosyl-valyl- alanyl-aspartyl chloromethyl ketone, an inhibitor of the cysteine protease caspase-1. The expression levels of NLRP3, apoptosis-associated speck-like protein containing A CARD (ASC) and caspase-1, and the release of interleukin (IL)-1β and IL-18 were measured. The results of the present study indicated that HG increased the expression levels of NLRP3, ASC and cleaved caspase-1, and the release of IL-1β and IL-18 in adipocytes. Caspase-1 inhibition abolished HG-induced production of IL-1β and IL-18 in adipocytes. Furthermore, NaHS inhibited the expression of NLRP3, ASC and cleaved caspase-1, and the production of IL-1β and IL-18 in adipocytes treated with HG. In conclusion, HG may increase and exogenous H2S may inhibit HG-induced NLRP3 inflammasome activation in adipocytes.

Authors+Show Affiliations

Department of Endocrinology, Chinese People's Liberation Army 903 Hospital, Hangzhou, Zhejiang 310013, P.R. China.Department of Physiology, Naval Medical University, Shanghai 200433, P.R. China.Department of Endocrinology, Chinese People's Liberation Army 903 Hospital, Hangzhou, Zhejiang 310013, P.R. China.Department of Endocrinology, Chinese People's Liberation Army 903 Hospital, Hangzhou, Zhejiang 310013, P.R. China.Department of Endocrinology, Chinese People's Liberation Army 903 Hospital, Hangzhou, Zhejiang 310013, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31885713

Citation

Hu, Tian-Xiao, et al. "Hydrogen Sulfide Modulates High Glucose-induced NLRP3 Inflammasome Activation in 3T3-L1 Adipocytes." Experimental and Therapeutic Medicine, vol. 19, no. 1, 2020, pp. 771-776.
Hu TX, Zhang NN, Ruan Y, et al. Hydrogen sulfide modulates high glucose-induced NLRP3 inflammasome activation in 3T3-L1 adipocytes. Exp Ther Med. 2020;19(1):771-776.
Hu, T. X., Zhang, N. N., Ruan, Y., Tan, Q. Y., & Wang, J. (2020). Hydrogen sulfide modulates high glucose-induced NLRP3 inflammasome activation in 3T3-L1 adipocytes. Experimental and Therapeutic Medicine, 19(1), pp. 771-776. doi:10.3892/etm.2019.8242.
Hu TX, et al. Hydrogen Sulfide Modulates High Glucose-induced NLRP3 Inflammasome Activation in 3T3-L1 Adipocytes. Exp Ther Med. 2020;19(1):771-776. PubMed PMID: 31885713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen sulfide modulates high glucose-induced NLRP3 inflammasome activation in 3T3-L1 adipocytes. AU - Hu,Tian-Xiao, AU - Zhang,Ning-Ning, AU - Ruan,Yun, AU - Tan,Qing-Ying, AU - Wang,Jing, Y1 - 2019/11/26/ PY - 2019/03/05/received PY - 2019/08/23/accepted PY - 2019/12/31/entrez PY - 2019/12/31/pubmed PY - 2019/12/31/medline KW - NACHT leucine rich repeat and pyd domains-containing 3 inflammasome KW - adipocyte KW - diabetes KW - hydrogen sulfide SP - 771 EP - 776 JF - Experimental and therapeutic medicine JO - Exp Ther Med VL - 19 IS - 1 N2 - Activation of the NACHT leucine rich repeat and pyd domains-containing 3 (NLRP3) inflammasome plays an important role in the initiation of inflammation in adipose tissue in diabetic patients. However, the mechanisms underlying this are not fully understood. Hydrogen sulfide (H2S) has been shown to have anti-inflammatory properties in various cell types. The present study aimed to investigate the effect of H2S on high glucose (HG)-induced NLRP3 inflammasome activation in adipocytes. Adipocytes were differentiated from 3T3-L1 cells and treated with low glucose (LG), HG, H2S donor sodium hydrosulfide (NaHS) or N-acetyl-tyrosyl-valyl- alanyl-aspartyl chloromethyl ketone, an inhibitor of the cysteine protease caspase-1. The expression levels of NLRP3, apoptosis-associated speck-like protein containing A CARD (ASC) and caspase-1, and the release of interleukin (IL)-1β and IL-18 were measured. The results of the present study indicated that HG increased the expression levels of NLRP3, ASC and cleaved caspase-1, and the release of IL-1β and IL-18 in adipocytes. Caspase-1 inhibition abolished HG-induced production of IL-1β and IL-18 in adipocytes. Furthermore, NaHS inhibited the expression of NLRP3, ASC and cleaved caspase-1, and the production of IL-1β and IL-18 in adipocytes treated with HG. In conclusion, HG may increase and exogenous H2S may inhibit HG-induced NLRP3 inflammasome activation in adipocytes. SN - 1792-0981 UR - https://www.unboundmedicine.com/medline/citation/31885713/Hydrogen_sulfide_modulates_high_glucose-induced_NLRP3_inflammasome_activation_in_3T3-L1_adipocytes DB - PRIME DP - Unbound Medicine ER -
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