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Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers.
Dis Markers. 2019; 2019:5292787.DM

Abstract

Background

Lung squamous cell carcinoma (LUSC) is a subtype of highly malignant lung cancer with poor prognosis, for which smoking is the main risk factor. However, the underlying genetic and molecular mechanisms of smoking-related LUSC remain largely unknown.

Methods

We mined existing LUSC-related mRNA, miRNA, and lncRNA transcriptome data and corresponding clinical data from The Cancer Genome Atlas (TCGA) database and divided them into smoking and nonsmoking groups, followed by differential expression analysis. Functional enrichment analysis of the unique differentially expressed mRNAs of the two groups was performed using the DAVID database. Subsequently, the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network of LUSC in smoking and nonsmoking groups was constructed. Finally, survival analyses were performed to determine the effects of differentially expressed lncRNAs/mRNAs/miRNAs that were involved in the ceRNA network on overall survival and to discover the hub genes.

Results

A total of 1696 lncRNAs, 125 miRNAs, and 3246 mRNAs and 1784 lncRNAs, 96 miRNAs, and 3229 mRNAs with differentially expressed profiles were identified in the smoking and nonsmoking groups, respectively. The ceRNA network and survival analysis revealed four lncRNAs (LINC00466, DLX6-AS1, LINC00261, and AGBL1), one miRNA (hsa-mir-210), and two mRNAs (CITED2 and ENPP4), with the potential as biomarkers for smoking-related LUSC diagnosis and prognosis.

Conclusion

Taken together, our research has identified the differences in the ceRNA regulatory networks between smoking and nonsmoking LUSC, which could lay the foundation for future clinical research.

Authors+Show Affiliations

Clinical Medical Colleges, Weifang Medical University, No. 7166, Baotong Western Street, Weifang, Shandong Province, China.College of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, No. 16369, Jingshi Road, Jinan, Shandong Province, China.College of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, No. 16369, Jingshi Road, Jinan, Shandong Province, China.Department of Oncology, Weifang Traditional Chinese Hospital, No. 1055, Weizhou Road, Weifang, Shandong Province, China.Department of Oncology, Weifang Traditional Chinese Hospital, No. 1055, Weizhou Road, Weifang, Shandong Province, China.Medical Colleges, Qingdao University, No. 308, Ningxia Road, Shinan District, Qingdao, Shandong Province, China.College of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, No. 16369, Jingshi Road, Jinan, Shandong Province, China.Clinical Medical Colleges, Weifang Medical University, No. 7166, Baotong Western Street, Weifang, Shandong Province, China.Department of Oncology, Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Shinan District, Qingdao, Shandong Province, China. Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250014 Shandong, China.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

31885738

Citation

Yao, Yan, et al. "Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers." Disease Markers, vol. 2019, 2019, p. 5292787.
Yao Y, Zhang T, Qi L, et al. Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers. Dis Markers. 2019;2019:5292787.
Yao, Y., Zhang, T., Qi, L., Liu, R., Liu, G., Wang, X., Li, J., Li, J., & Sun, C. (2019). Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers. Disease Markers, 2019, 5292787. https://doi.org/10.1155/2019/5292787
Yao Y, et al. Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers. Dis Markers. 2019;2019:5292787. PubMed PMID: 31885738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Competitive Endogenous RNA Network Construction and Comparison of Lung Squamous Cell Carcinoma in Smokers and Nonsmokers. AU - Yao,Yan, AU - Zhang,Tingting, AU - Qi,Lingyu, AU - Liu,Ruijuan, AU - Liu,Gongxi, AU - Wang,Xue, AU - Li,Jie, AU - Li,Jia, AU - Sun,Changgang, Y1 - 2019/12/04/ PY - 2019/05/27/received PY - 2019/10/19/revised PY - 2019/11/14/accepted PY - 2019/12/31/entrez PY - 2019/12/31/pubmed PY - 2019/12/31/medline SP - 5292787 EP - 5292787 JF - Disease markers JO - Dis. Markers VL - 2019 N2 - Background: Lung squamous cell carcinoma (LUSC) is a subtype of highly malignant lung cancer with poor prognosis, for which smoking is the main risk factor. However, the underlying genetic and molecular mechanisms of smoking-related LUSC remain largely unknown. Methods: We mined existing LUSC-related mRNA, miRNA, and lncRNA transcriptome data and corresponding clinical data from The Cancer Genome Atlas (TCGA) database and divided them into smoking and nonsmoking groups, followed by differential expression analysis. Functional enrichment analysis of the unique differentially expressed mRNAs of the two groups was performed using the DAVID database. Subsequently, the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network of LUSC in smoking and nonsmoking groups was constructed. Finally, survival analyses were performed to determine the effects of differentially expressed lncRNAs/mRNAs/miRNAs that were involved in the ceRNA network on overall survival and to discover the hub genes. Results: A total of 1696 lncRNAs, 125 miRNAs, and 3246 mRNAs and 1784 lncRNAs, 96 miRNAs, and 3229 mRNAs with differentially expressed profiles were identified in the smoking and nonsmoking groups, respectively. The ceRNA network and survival analysis revealed four lncRNAs (LINC00466, DLX6-AS1, LINC00261, and AGBL1), one miRNA (hsa-mir-210), and two mRNAs (CITED2 and ENPP4), with the potential as biomarkers for smoking-related LUSC diagnosis and prognosis. Conclusion: Taken together, our research has identified the differences in the ceRNA regulatory networks between smoking and nonsmoking LUSC, which could lay the foundation for future clinical research. SN - 1875-8630 UR - https://www.unboundmedicine.com/medline/citation/31885738/Competitive_Endogenous_RNA_Network_Construction_and_Comparison_of_Lung_Squamous_Cell_Carcinoma_in_Smokers_and_Nonsmokers_ L2 - https://doi.org/10.1155/2019/5292787 DB - PRIME DP - Unbound Medicine ER -