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Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis.
Acta Trop. 2020 Mar; 203:105325.AT

Abstract

Paragonimiasis, an important food-borne zoonosis, is caused by lung flukes of the genus Paragonimus. Several million people are actually infected or at risk. Paragonimiasis is a re-emerging disease in developing countries. Diagnosis of pulmonary paragonimiasis is made by finding eggs in sputa and/or fecal samples. Eggs are typically not found in ectopic paragonimiasis cases, so diagnosis depends on supportive information, such as a history of eating fresh water crabs or crayfishes, radiographic findings and immunological tests. Here, a new point-of-care-testing (POCT) tool is presented. It uses immunochromatography for serodiagnosis of human paragonimiasis using excretory-secretory antigen from Paragonimus heterotremus. It proved effective in diagnosing infections due to P. heterotremus, and was also successfully diagnosed with sera from infections with P. westermani and P. miyazakii. The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy were 97.9%, 87.6%, 78%, 98.9%, and 90.8%, respectively. The developed POCT tool is rapid and simple to use not only for clinical diagnosis of paragonimiasis at the bedside or at well-equipped laboratories, but also at local and remote hospitals with limited facilities. Moreover, the POCT tool could be applied for epidemiological surveys of paragonimaisis in Asia where P. heterotremus, P. westermani and P. miyazakii are endemic.

Authors+Show Affiliations

Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.Department of Parasitology, National Institute of Infectious Diseases, Ministry of Health, Labour and Welfare, Tokyo, Japan.Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand. Electronic address: pewpan@kku.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31891708

Citation

Sadaow, Lakkhana, et al. "Development of Point-of-care Testing Tool Using Immunochromatography for Rapid Diagnosis of Human Paragonimiasis." Acta Tropica, vol. 203, 2020, p. 105325.
Sadaow L, Sanpool O, Yamasaki H, et al. Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis. Acta Trop. 2020;203:105325.
Sadaow, L., Sanpool, O., Yamasaki, H., Maleewong, W., & Intapan, P. M. (2020). Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis. Acta Tropica, 203, 105325. https://doi.org/10.1016/j.actatropica.2019.105325
Sadaow L, et al. Development of Point-of-care Testing Tool Using Immunochromatography for Rapid Diagnosis of Human Paragonimiasis. Acta Trop. 2020;203:105325. PubMed PMID: 31891708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis. AU - Sadaow,Lakkhana, AU - Sanpool,Oranuch, AU - Yamasaki,Hiroshi, AU - Maleewong,Wanchai, AU - Intapan,Pewpan M, Y1 - 2019/12/28/ PY - 2019/08/02/received PY - 2019/12/24/revised PY - 2019/12/27/accepted PY - 2020/1/1/pubmed PY - 2020/1/1/medline PY - 2020/1/1/entrez KW - Immunochromatography KW - Paragonimiasis KW - Paragonimus heterotremus KW - Paragonimus miyazakii KW - Paragonimus westermani KW - Serodiagnosis SP - 105325 EP - 105325 JF - Acta tropica JO - Acta Trop. VL - 203 N2 - Paragonimiasis, an important food-borne zoonosis, is caused by lung flukes of the genus Paragonimus. Several million people are actually infected or at risk. Paragonimiasis is a re-emerging disease in developing countries. Diagnosis of pulmonary paragonimiasis is made by finding eggs in sputa and/or fecal samples. Eggs are typically not found in ectopic paragonimiasis cases, so diagnosis depends on supportive information, such as a history of eating fresh water crabs or crayfishes, radiographic findings and immunological tests. Here, a new point-of-care-testing (POCT) tool is presented. It uses immunochromatography for serodiagnosis of human paragonimiasis using excretory-secretory antigen from Paragonimus heterotremus. It proved effective in diagnosing infections due to P. heterotremus, and was also successfully diagnosed with sera from infections with P. westermani and P. miyazakii. The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy were 97.9%, 87.6%, 78%, 98.9%, and 90.8%, respectively. The developed POCT tool is rapid and simple to use not only for clinical diagnosis of paragonimiasis at the bedside or at well-equipped laboratories, but also at local and remote hospitals with limited facilities. Moreover, the POCT tool could be applied for epidemiological surveys of paragonimaisis in Asia where P. heterotremus, P. westermani and P. miyazakii are endemic. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/31891708/Development_of_point-of-care_testing_tool_using_immunochromatography_for_rapid_diagnosis_of_human_paragonimiasis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(19)31027-7 DB - PRIME DP - Unbound Medicine ER -
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