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Evaluation of molecular typing and serological methods in solving discrepant results of weak and partial D (Rh) in South Egypt.
Asian J Transfus Sci 2019 Jul-Dec; 13(2):110-114AJ

Abstract

INTRODUCTION

Rh discrepancies produced by partial and weak D phenotypes are a problem during routine testing. Some blood units with weak and partial D expression may be missed by serology. Overcoming the limitations of serology can be achieved by molecular typing. Our objective was to evaluate currently used serologic methods with the molecular analysis in solving discrepant results of weak and partial D (Rh) in South Egypt.

PATIENTS AND METHODS

Fifty blood donor and patient samples with undetermined D phenotype were subjected to serology to define their phenotype using identification (ID)-Card "ID-partial RhD typing set" using six monoclonal anti-D panels, followed by molecular typing using polymerase chain reaction sequence-specific primer kit.

RESULTS

Molecular typing confirmed most of the serology results; two samples previously resolved as partial D Type 3 and DFR by serological methods were clarified by molecular techniques - one sample as weak Type 4 and the other sample as weak Type 3. Among the weak D alleles found in our study, Type 4 was the most common, with a frequency of 20%, followed by Type 3 (14%), Type 1 (8%), Type 2 (6%), and finally, Type 5 with a frequency of 3%. The most common types of partial D were partial D Type D5 (14%) and Type D3 (10%).

CONCLUSION

Our study identified D variants (weak D and partial D categories) of the antigen D and determined the frequency and composition of partial D and weak D alleles in our population. Molecular typing also confirmed most of the results obtained from serological methods.

Authors+Show Affiliations

Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31896917

Citation

Bakry, Rania M., et al. "Evaluation of Molecular Typing and Serological Methods in Solving Discrepant Results of Weak and Partial D (Rh) in South Egypt." Asian Journal of Transfusion Science, vol. 13, no. 2, 2019, pp. 110-114.
Bakry RM, Nasreldin E, Hassaballa AE, et al. Evaluation of molecular typing and serological methods in solving discrepant results of weak and partial D (Rh) in South Egypt. Asian J Transfus Sci. 2019;13(2):110-114.
Bakry, R. M., Nasreldin, E., Hassaballa, A. E., Mansour, S. M., & Aboalia, S. A. (2019). Evaluation of molecular typing and serological methods in solving discrepant results of weak and partial D (Rh) in South Egypt. Asian Journal of Transfusion Science, 13(2), pp. 110-114. doi:10.4103/ajts.AJTS_162_18.
Bakry RM, et al. Evaluation of Molecular Typing and Serological Methods in Solving Discrepant Results of Weak and Partial D (Rh) in South Egypt. Asian J Transfus Sci. 2019;13(2):110-114. PubMed PMID: 31896917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of molecular typing and serological methods in solving discrepant results of weak and partial D (Rh) in South Egypt. AU - Bakry,Rania M, AU - Nasreldin,Eman, AU - Hassaballa,Ashraf E, AU - Mansour,Samar M, AU - Aboalia,Sahar A, Y1 - 2019/12/03/ PY - 2018/12/26/received PY - 2019/03/31/accepted PY - 2020/1/4/entrez PY - 2020/1/4/pubmed PY - 2020/1/4/medline KW - Partial RhD KW - Rh genotypes KW - Rh phenotypes KW - Weak RhD SP - 110 EP - 114 JF - Asian journal of transfusion science JO - Asian J Transfus Sci VL - 13 IS - 2 N2 - INTRODUCTION: Rh discrepancies produced by partial and weak D phenotypes are a problem during routine testing. Some blood units with weak and partial D expression may be missed by serology. Overcoming the limitations of serology can be achieved by molecular typing. Our objective was to evaluate currently used serologic methods with the molecular analysis in solving discrepant results of weak and partial D (Rh) in South Egypt. PATIENTS AND METHODS: Fifty blood donor and patient samples with undetermined D phenotype were subjected to serology to define their phenotype using identification (ID)-Card "ID-partial RhD typing set" using six monoclonal anti-D panels, followed by molecular typing using polymerase chain reaction sequence-specific primer kit. RESULTS: Molecular typing confirmed most of the serology results; two samples previously resolved as partial D Type 3 and DFR by serological methods were clarified by molecular techniques - one sample as weak Type 4 and the other sample as weak Type 3. Among the weak D alleles found in our study, Type 4 was the most common, with a frequency of 20%, followed by Type 3 (14%), Type 1 (8%), Type 2 (6%), and finally, Type 5 with a frequency of 3%. The most common types of partial D were partial D Type D5 (14%) and Type D3 (10%). CONCLUSION: Our study identified D variants (weak D and partial D categories) of the antigen D and determined the frequency and composition of partial D and weak D alleles in our population. Molecular typing also confirmed most of the results obtained from serological methods. SN - 0973-6247 UR - https://www.unboundmedicine.com/medline/citation/31896917/Evaluation_of_molecular_typing_and_serological_methods_in_solving_discrepant_results_of_weak_and_partial_D_(Rh)_in_South_Egypt L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31896917/ DB - PRIME DP - Unbound Medicine ER -