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Comparative studies on tree pollen allergens. XIV. Characterization of the birch (Betula verrucosa) and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography.
Ann Allergy. 1988 Nov; 61(5):392-400.AA

Abstract

The crude aqueous extracts of birch (Betula verrucosa) and hazel (Corylus avellana) pollen were characterized by horizontal isoelectric focusing (IEF) and one-dimensional and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1-D and 2-D SDS/PAGE). Isoelectric focusing reference patterns obtained in pH 3.5 to 9.5 gradient gels contained 31 and 41 bands while the 2-D patterns consisted of 51 and 72 protein spots, respectively. Electrophoretic transfer to nitrocellulose membranes and subsequent 125I-immunoautoradiography demonstrated IgE binding in the region between pH 4.8 and 5.4 for both extracts. The main allergenic reactivities of this pH region were distributed by SDS/PAGE to the molecular weights of 29.5, 17, and 12.5 kilodaltons for birch and 15.5 and 12 kilodaltons for hazel. The high resolving power and the independence of rabbit antibodies were considered the major advantages of the described 2-D methodology as compared with conventional crossed immunoelectrophoretic and radioimmunoelectrophoretic techniques.

Authors+Show Affiliations

Florvaag E
Laboratory of Clinical Biochemistry, Haukeland University Hospital, Bergen, Norway.
Holen E
No affiliation info available
Vik H
No affiliation info available
Elsayed S
No affiliation info available

MeSH

AnimalsAutoradiographyElectrophoresis, Polyacrylamide GelHumansImmunoelectrophoresis, Two-DimensionalImmunoglobulin EIsoelectric FocusingPlant ExtractsPollenRabbitsRadioimmunoassaySodium Dodecyl SulfateSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3189966

Citation

Florvaag, E, et al. "Comparative Studies On Tree Pollen Allergens. XIV. Characterization of the Birch (Betula Verrucosa) and Hazel (Corylus Avellana) Pollen Extracts By Horizontal 2-D SDS-PAGE Combined With Electrophoretic Transfer and IgE Immunoautoradiography." Annals of Allergy, vol. 61, no. 5, 1988, pp. 392-400.
Florvaag E, Holen E, Vik H, et al. Comparative studies on tree pollen allergens. XIV. Characterization of the birch (Betula verrucosa) and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography. Ann Allergy. 1988;61(5):392-400.
Florvaag, E., Holen, E., Vik, H., & Elsayed, S. (1988). Comparative studies on tree pollen allergens. XIV. Characterization of the birch (Betula verrucosa) and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography. Annals of Allergy, 61(5), 392-400.
Florvaag E, et al. Comparative Studies On Tree Pollen Allergens. XIV. Characterization of the Birch (Betula Verrucosa) and Hazel (Corylus Avellana) Pollen Extracts By Horizontal 2-D SDS-PAGE Combined With Electrophoretic Transfer and IgE Immunoautoradiography. Ann Allergy. 1988;61(5):392-400. PubMed PMID: 3189966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative studies on tree pollen allergens. XIV. Characterization of the birch (Betula verrucosa) and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography. AU - Florvaag,E, AU - Holen,E, AU - Vik,H, AU - Elsayed,S, PY - 1988/11/1/pubmed PY - 1988/11/1/medline PY - 1988/11/1/entrez SP - 392 EP - 400 JF - Annals of allergy JO - Ann Allergy VL - 61 IS - 5 N2 - The crude aqueous extracts of birch (Betula verrucosa) and hazel (Corylus avellana) pollen were characterized by horizontal isoelectric focusing (IEF) and one-dimensional and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1-D and 2-D SDS/PAGE). Isoelectric focusing reference patterns obtained in pH 3.5 to 9.5 gradient gels contained 31 and 41 bands while the 2-D patterns consisted of 51 and 72 protein spots, respectively. Electrophoretic transfer to nitrocellulose membranes and subsequent 125I-immunoautoradiography demonstrated IgE binding in the region between pH 4.8 and 5.4 for both extracts. The main allergenic reactivities of this pH region were distributed by SDS/PAGE to the molecular weights of 29.5, 17, and 12.5 kilodaltons for birch and 15.5 and 12 kilodaltons for hazel. The high resolving power and the independence of rabbit antibodies were considered the major advantages of the described 2-D methodology as compared with conventional crossed immunoelectrophoretic and radioimmunoelectrophoretic techniques. SN - 0003-4738 UR - https://www.unboundmedicine.com/medline/citation/3189966/Comparative_studies_on_tree_pollen_allergens__XIV__Characterization_of_the_birch__Betula_verrucosa__and_hazel__Corylus_avellana__pollen_extracts_by_horizontal_2_D_SDS_PAGE_combined_with_electrophoretic_transfer_and_IgE_immunoautoradiography_ DB - PRIME DP - Unbound Medicine ER -

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