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Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination.
PLoS One. 2020; 15(1):e0219413.Plos

Abstract

Seed dormancy and germination are the two important traits related to plant survival, reproduction and crop yield. To understand the regulatory mechanisms of these traits, it is crucial to clarify which genes or pathways participate in the regulation of these processes. However, little information is available on seed dormancy and germination in peanut. In this study, seeds of the variety Luhua No.14, which undergoes nondeep dormancy, were selected, and their transcriptional changes at three different developmental stages, the freshly harvested seed (FS), the after-ripening seed (DS) and the newly germinated seed (GS) stages, were investigated by comparative transcriptomic analysis. The results showed that genes with increased transcription in the DS vs FS comparison were overrepresented for oxidative phosphorylation, the glycolysis pathway and the tricarboxylic acid (TCA) cycle, suggesting that after a period of dry storage, the intermediates stored in the dry seeds were rapidly mobilized by glycolysis, the TCA cycle, the glyoxylate cycle, etc.; the electron transport chain accompanied by respiration was reactivated to provide ATP for the mobilization of other reserves and for seed germination. In the GS vs DS pairwise comparison, dozens of the upregulated genes were related to plant hormone biosynthesis and signal transduction, including the majority of components involved in the auxin signal pathway, brassinosteroid biosynthesis and signal transduction as well as some GA and ABA signal transduction genes. During seed germination, the expression of some EXPANSIN and XYLOGLUCAN ENDOTRANSGLYCOSYLASE genes was also significantly enhanced. To investigate the effects of different hormones during seed germination, the contents and differential distribution of ABA, GAs, BRs and IAA in the cotyledons, hypocotyls and radicles, and plumules of three seed sections at different developmental stages were also investigated. Combined with previous data in other species, it was suggested that the coordination of multiple hormone signal transduction nets plays a key role in radicle protrusion and seed germination.

Authors+Show Affiliations

Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China. College of Life Science, Shandong Normal University, Jinan, Shandong, China.Shandong Academy of Grape, Jinan, Shandong, China.College of Life Science, Shandong Normal University, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China. College of Life Science, Shandong Normal University, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China. College of Life Science, Shandong Normal University, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China. College of Life Science, Shandong Normal University, Jinan, Shandong, China.Shandong Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.Bio-Tech Research Center, Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, Shandong, China. College of Life Science, Shandong Normal University, Jinan, Shandong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31899920

Citation

Xu, Pingli, et al. "Transcriptional Differences in Peanut (Arachis Hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights Into the Regulatory Networks of Seed Dormancy Release and Germination." PloS One, vol. 15, no. 1, 2020, pp. e0219413.
Xu P, Tang G, Cui W, et al. Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination. PLoS ONE. 2020;15(1):e0219413.
Xu, P., Tang, G., Cui, W., Chen, G., Ma, C. L., Zhu, J., Li, P., Shan, L., Liu, Z., & Wan, S. (2020). Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination. PloS One, 15(1), e0219413. https://doi.org/10.1371/journal.pone.0219413
Xu P, et al. Transcriptional Differences in Peanut (Arachis Hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights Into the Regulatory Networks of Seed Dormancy Release and Germination. PLoS ONE. 2020;15(1):e0219413. PubMed PMID: 31899920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination. AU - Xu,Pingli, AU - Tang,Guiying, AU - Cui,Weipei, AU - Chen,Guangxia, AU - Ma,Chang-Le, AU - Zhu,Jieqiong, AU - Li,Pengxiang, AU - Shan,Lei, AU - Liu,Zhanji, AU - Wan,Shubo, Y1 - 2020/01/03/ PY - 2019/07/18/received PY - 2019/12/05/accepted PY - 2020/1/4/entrez PY - 2020/1/4/pubmed PY - 2020/1/4/medline SP - e0219413 EP - e0219413 JF - PloS one JO - PLoS ONE VL - 15 IS - 1 N2 - Seed dormancy and germination are the two important traits related to plant survival, reproduction and crop yield. To understand the regulatory mechanisms of these traits, it is crucial to clarify which genes or pathways participate in the regulation of these processes. However, little information is available on seed dormancy and germination in peanut. In this study, seeds of the variety Luhua No.14, which undergoes nondeep dormancy, were selected, and their transcriptional changes at three different developmental stages, the freshly harvested seed (FS), the after-ripening seed (DS) and the newly germinated seed (GS) stages, were investigated by comparative transcriptomic analysis. The results showed that genes with increased transcription in the DS vs FS comparison were overrepresented for oxidative phosphorylation, the glycolysis pathway and the tricarboxylic acid (TCA) cycle, suggesting that after a period of dry storage, the intermediates stored in the dry seeds were rapidly mobilized by glycolysis, the TCA cycle, the glyoxylate cycle, etc.; the electron transport chain accompanied by respiration was reactivated to provide ATP for the mobilization of other reserves and for seed germination. In the GS vs DS pairwise comparison, dozens of the upregulated genes were related to plant hormone biosynthesis and signal transduction, including the majority of components involved in the auxin signal pathway, brassinosteroid biosynthesis and signal transduction as well as some GA and ABA signal transduction genes. During seed germination, the expression of some EXPANSIN and XYLOGLUCAN ENDOTRANSGLYCOSYLASE genes was also significantly enhanced. To investigate the effects of different hormones during seed germination, the contents and differential distribution of ABA, GAs, BRs and IAA in the cotyledons, hypocotyls and radicles, and plumules of three seed sections at different developmental stages were also investigated. Combined with previous data in other species, it was suggested that the coordination of multiple hormone signal transduction nets plays a key role in radicle protrusion and seed germination. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31899920/Transcriptional_Differences_in_Peanut_(Arachis_hypogaea_L.)_Seeds_at_the_Freshly_Harvested,_After-ripening_and_Newly_Germinated_Seed_Stages:_Insights_into_the_Regulatory_Networks_of_Seed_Dormancy_Release_and_Germination L2 - http://dx.plos.org/10.1371/journal.pone.0219413 DB - PRIME DP - Unbound Medicine ER -
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