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Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt.
Vet Microbiol. 2020 Jan; 240:108535.VM

Abstract

Methicillin resistant S. aureus from cows with mastitis has received a growing interest worldwide. The present study aimed to provide a detailed description of the resistance and virulence traits of isolates from bovine mastitis samples. A total of 550 quarter milk samples were collected from 140 mastitic household dairy cows and buffalo from five herds at Dakahlia Governorate, Egypt, during 2017 and 2018. Staphylococcus spp. were isolated and differentiated using MALDI-TOF MS. A genotypic characterization was performed for S. aureus isolates using DNA-microarray and staphylococcal protein A (spa) typing. Furthermore, antibiotic resistances were phenotypically confirmed using broth microdilution. Six different clonal lineages (CC1-MRSA, CC5-MRSA, CC45-MRSA, CC97-MSSA, CC50-MSSA and CC1153-MSSA), including seven spa types (t127, t688, t132, t267, t521, t224 and t903) were identified. Spa type t267 was the most dominant among the investigated herds. This is the first report of the occurrence of clonal lineages CC97, CC1, CC45, CC50 and CC1153 from bovine mastitis in Egypt. All MRSA isolates and 33.3 % of MSSA were multi-resistant (i.e. resistant to more than three classes of compounds). Various virulence determinants were also observed including leukocidins, hemolysins, and enterotoxins. The study demonstrates a low diversity of S. aureus isolates recovered from several dairy herds. The findings of the observed virulotypes can be useful for future studies on anti-virulence therapies, immunogenicity and vaccine development.

Authors+Show Affiliations

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: magid_rez@mans.edu.eg.Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, 35516 Egypt.Leibniz-Institute of Photonic Technology (IPHT), Department for Optical Molecular Diagnostics and System Technology, Jena, Germany; InfectoGnostics Research Campus, Centre for Applied Research, Jena, Germany; Institute for Medical Microbiology and Hygiene, Medical Faculty "Carl Gustav Carus", Technische Universität Dresden, Dresden, Germany.Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, 35516 Egypt.Leibniz-Institute of Photonic Technology (IPHT), Department for Optical Molecular Diagnostics and System Technology, Jena, Germany; InfectoGnostics Research Campus, Centre for Applied Research, Jena, Germany; Friedrich Schiller University, Institute of Physical Chemistry, Jena, Germany.Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, 35516 Egypt.Microbiology Unit, Department of Biological Sciences, Covenant University, Otta, Nigeria.Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, 35516 Egypt.German Federal Institute for Risk Assessment, Department of Biological Safety, Berlin, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31902507

Citation

El-Ashker, Maged, et al. "Antimicrobial Resistance Pattern and Virulence Profile of S. Aureus Isolated From Household Cattle and Buffalo With Mastitis in Egypt." Veterinary Microbiology, vol. 240, 2020, p. 108535.
El-Ashker M, Gwida M, Monecke S, et al. Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt. Vet Microbiol. 2020;240:108535.
El-Ashker, M., Gwida, M., Monecke, S., El-Gohary, F., Ehricht, R., Elsayed, M., Akinduti, P., El-Fateh, M., & Maurischat, S. (2020). Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt. Veterinary Microbiology, 240, 108535. https://doi.org/10.1016/j.vetmic.2019.108535
El-Ashker M, et al. Antimicrobial Resistance Pattern and Virulence Profile of S. Aureus Isolated From Household Cattle and Buffalo With Mastitis in Egypt. Vet Microbiol. 2020;240:108535. PubMed PMID: 31902507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt. AU - El-Ashker,Maged, AU - Gwida,Mayada, AU - Monecke,Stefan, AU - El-Gohary,Fatma, AU - Ehricht,Ralf, AU - Elsayed,Mona, AU - Akinduti,Paul, AU - El-Fateh,Mohamed, AU - Maurischat,Sven, Y1 - 2019/11/29/ PY - 2019/06/21/received PY - 2019/10/25/revised PY - 2019/11/28/accepted PY - 2020/1/7/entrez PY - 2020/1/7/pubmed PY - 2020/9/17/medline KW - Antimicrobial resistance KW - Bovine mastitis KW - DNA-Microarray KW - Egypt KW - S. aureus KW - Virulence genes SP - 108535 EP - 108535 JF - Veterinary microbiology JO - Vet Microbiol VL - 240 N2 - Methicillin resistant S. aureus from cows with mastitis has received a growing interest worldwide. The present study aimed to provide a detailed description of the resistance and virulence traits of isolates from bovine mastitis samples. A total of 550 quarter milk samples were collected from 140 mastitic household dairy cows and buffalo from five herds at Dakahlia Governorate, Egypt, during 2017 and 2018. Staphylococcus spp. were isolated and differentiated using MALDI-TOF MS. A genotypic characterization was performed for S. aureus isolates using DNA-microarray and staphylococcal protein A (spa) typing. Furthermore, antibiotic resistances were phenotypically confirmed using broth microdilution. Six different clonal lineages (CC1-MRSA, CC5-MRSA, CC45-MRSA, CC97-MSSA, CC50-MSSA and CC1153-MSSA), including seven spa types (t127, t688, t132, t267, t521, t224 and t903) were identified. Spa type t267 was the most dominant among the investigated herds. This is the first report of the occurrence of clonal lineages CC97, CC1, CC45, CC50 and CC1153 from bovine mastitis in Egypt. All MRSA isolates and 33.3 % of MSSA were multi-resistant (i.e. resistant to more than three classes of compounds). Various virulence determinants were also observed including leukocidins, hemolysins, and enterotoxins. The study demonstrates a low diversity of S. aureus isolates recovered from several dairy herds. The findings of the observed virulotypes can be useful for future studies on anti-virulence therapies, immunogenicity and vaccine development. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/31902507/Antimicrobial_resistance_pattern_and_virulence_profile_of_S__aureus_isolated_from_household_cattle_and_buffalo_with_mastitis_in_Egypt_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(19)30733-3 DB - PRIME DP - Unbound Medicine ER -