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Identification and quantification of ethylene glycol and diethylene glycol in plasma using gas chromatography-mass spectrometry.
Arch Toxicol. 1988 Aug; 62(1):66-9.AT

Abstract

A method for the gas chromatographic-mass spectrometric identification and quantification of ethylene glycol and diethylene glycol in plasma is described. Such a method is necessary in clinical and forensic toxicology to diagnose probable intoxication and to control the efficacy of detoxification. For sample preparation, the glycols were isolated using acetone after the addition of 1,3-propylene glycol as internal standard. The glycols were then esterified by pivalic acid (pivalic acid anhydride, triethylamine and methanol, 70 degrees C, 15 min) to improve their gas chromatographic characteristics. The glycols were first identified by a comparison of the full mass spectra with reference spectra and then quantified. Therefore, the peak area ratio in the total ion chromatogram (ethylene glycol or diethylene glycol/1,3-propanediol) of the sample was compared with the calibration curve in which the peak area ratios of the standards (0.05, 0.1, 0.5, 1 and 2 g/l), prepared in the same way, were plotted versus their concentrations. The method was linear at least from 0.05 to 2 g/l, with a detection limit of less than 0.01 g/l. The analytical recoveries were 99.2-102.9% for the different concentrations. Precision studies show coefficients of variation of 3.0-6.3% for the different concentrations.

Authors+Show Affiliations

Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg (Saar), Federal Republic of Germany.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3190459

Citation

Maurer, H, and C Kessler. "Identification and Quantification of Ethylene Glycol and Diethylene Glycol in Plasma Using Gas Chromatography-mass Spectrometry." Archives of Toxicology, vol. 62, no. 1, 1988, pp. 66-9.
Maurer H, Kessler C. Identification and quantification of ethylene glycol and diethylene glycol in plasma using gas chromatography-mass spectrometry. Arch Toxicol. 1988;62(1):66-9.
Maurer, H., & Kessler, C. (1988). Identification and quantification of ethylene glycol and diethylene glycol in plasma using gas chromatography-mass spectrometry. Archives of Toxicology, 62(1), 66-9.
Maurer H, Kessler C. Identification and Quantification of Ethylene Glycol and Diethylene Glycol in Plasma Using Gas Chromatography-mass Spectrometry. Arch Toxicol. 1988;62(1):66-9. PubMed PMID: 3190459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and quantification of ethylene glycol and diethylene glycol in plasma using gas chromatography-mass spectrometry. AU - Maurer,H, AU - Kessler,C, PY - 1988/8/1/pubmed PY - 1988/8/1/medline PY - 1988/8/1/entrez SP - 66 EP - 9 JF - Archives of toxicology JO - Arch Toxicol VL - 62 IS - 1 N2 - A method for the gas chromatographic-mass spectrometric identification and quantification of ethylene glycol and diethylene glycol in plasma is described. Such a method is necessary in clinical and forensic toxicology to diagnose probable intoxication and to control the efficacy of detoxification. For sample preparation, the glycols were isolated using acetone after the addition of 1,3-propylene glycol as internal standard. The glycols were then esterified by pivalic acid (pivalic acid anhydride, triethylamine and methanol, 70 degrees C, 15 min) to improve their gas chromatographic characteristics. The glycols were first identified by a comparison of the full mass spectra with reference spectra and then quantified. Therefore, the peak area ratio in the total ion chromatogram (ethylene glycol or diethylene glycol/1,3-propanediol) of the sample was compared with the calibration curve in which the peak area ratios of the standards (0.05, 0.1, 0.5, 1 and 2 g/l), prepared in the same way, were plotted versus their concentrations. The method was linear at least from 0.05 to 2 g/l, with a detection limit of less than 0.01 g/l. The analytical recoveries were 99.2-102.9% for the different concentrations. Precision studies show coefficients of variation of 3.0-6.3% for the different concentrations. SN - 0340-5761 UR - https://www.unboundmedicine.com/medline/citation/3190459/Identification_and_quantification_of_ethylene_glycol_and_diethylene_glycol_in_plasma_using_gas_chromatography_mass_spectrometry_ L2 - https://doi.org/10.1007/BF00316260 DB - PRIME DP - Unbound Medicine ER -
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