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SNHG1 promotes MPP+-induced cytotoxicity by regulating PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells via sponging miR-153-3p.
Biol Res. 2020 Jan 06; 53(1):1.BR

Abstract

BACKGROUND

Long non-coding RNA small molecule RNA host gene 1 (SNHG1) was previously identified to be relevant with Parkinson's disease (PD) pathogenesis. This work aims to further elucidate the regulatory networks of SNHG1 involved in PD.

METHODS

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloride (MPTP)-induced mice and 1-methyl-4-phenylpyridinium (MPP+)-treated SH-SY5Y cells were respectively constructed as the in vivo and in vitro PD models. Expression levels of SNHG1 and miR-153-3p were detected by qRT-PCR. Protein expression levels of phosphate and tension homology deleted on chromosome ten (PTEN) were measured by western blotting assay. Cell viability and apoptosis were determined by MTT and flow cytometry assays. The interactions among SNHG1, miR-153-3p and PTEN were identified by luciferase reporter assay, RNA immunoprecipitation, and/or RNA pull-down analysis.

RESULTS

Increased SNHG1 expression was found in midbrain of MPTP-induced PD mice and MPP+-treated SH-SY5Y cells. Overexpression of SNHG1 lowered viability and enhanced apoptosis in MPP+-treated SH-SY5Y cells. Moreover, SNHG1 acted as a molecular sponge to inhibit the expression of miR-153-3p. Furthermore, miR-153-3p-mediated suppression of MPP+-induced cytotoxicity was abated following SNHG1 up-regulation. Additionally, PTEN was identified as a direct target of miR-153-3p, and SNHG1 could serve as a competing endogenous RNA (ceRNA) of miR-153-3p to improve the expression of PTEN. Besides, enforced expression of PTEN displayed the similar functions as SNHG1 overexpression in regulating the viability and apoptosis of MPP+-treated SH-SY5Y cells. Finally, SNHG1 was found to activate PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells by targeting miR-153-3p.

CONCLUSION

SNHG1 aggravates MPP+-induced cellular toxicity in SH-SY5Y cells by regulating PTEN/AKT/mTOR signaling via sponging miR-153-3p, indicating the potential of SNHG1 as a promising therapeutic target for PD.

Authors+Show Affiliations

Department of Neurology, Huaihe Hospital of Henan University, No. 357 Ximen Street, Kaifeng, 475000, China.Department of Rehabilitation Medicine, Huaihe Hospital of Henan University, Kaifeng, 475000, China.Department of Neurology, Huaihe Hospital of Henan University, No. 357 Ximen Street, Kaifeng, 475000, China. vwbkwx@163.com.Department of Neurology, Huaihe Hospital of Henan University, No. 357 Ximen Street, Kaifeng, 475000, China. wuchunfangful@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31907031

Citation

Zhao, Jun, et al. "SNHG1 Promotes MPP+-induced Cytotoxicity By Regulating PTEN/AKT/mTOR Signaling Pathway in SH-SY5Y Cells Via Sponging MiR-153-3p." Biological Research, vol. 53, no. 1, 2020, p. 1.
Zhao J, Geng L, Chen Y, et al. SNHG1 promotes MPP+-induced cytotoxicity by regulating PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells via sponging miR-153-3p. Biol Res. 2020;53(1):1.
Zhao, J., Geng, L., Chen, Y., & Wu, C. (2020). SNHG1 promotes MPP+-induced cytotoxicity by regulating PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells via sponging miR-153-3p. Biological Research, 53(1), 1. https://doi.org/10.1186/s40659-019-0267-y
Zhao J, et al. SNHG1 Promotes MPP+-induced Cytotoxicity By Regulating PTEN/AKT/mTOR Signaling Pathway in SH-SY5Y Cells Via Sponging MiR-153-3p. Biol Res. 2020 Jan 6;53(1):1. PubMed PMID: 31907031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SNHG1 promotes MPP+-induced cytotoxicity by regulating PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells via sponging miR-153-3p. AU - Zhao,Jun, AU - Geng,Lijiao, AU - Chen,Yong, AU - Wu,Chunfang, Y1 - 2020/01/06/ PY - 2019/05/24/received PY - 2019/12/09/accepted PY - 2020/1/8/entrez PY - 2020/1/8/pubmed PY - 2020/4/16/medline KW - IncRNA KW - MPP+ KW - PTEN KW - Parkinson’s disease KW - SNHG1 KW - miR-153-3p SP - 1 EP - 1 JF - Biological research JO - Biol. Res. VL - 53 IS - 1 N2 - BACKGROUND: Long non-coding RNA small molecule RNA host gene 1 (SNHG1) was previously identified to be relevant with Parkinson's disease (PD) pathogenesis. This work aims to further elucidate the regulatory networks of SNHG1 involved in PD. METHODS: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloride (MPTP)-induced mice and 1-methyl-4-phenylpyridinium (MPP+)-treated SH-SY5Y cells were respectively constructed as the in vivo and in vitro PD models. Expression levels of SNHG1 and miR-153-3p were detected by qRT-PCR. Protein expression levels of phosphate and tension homology deleted on chromosome ten (PTEN) were measured by western blotting assay. Cell viability and apoptosis were determined by MTT and flow cytometry assays. The interactions among SNHG1, miR-153-3p and PTEN were identified by luciferase reporter assay, RNA immunoprecipitation, and/or RNA pull-down analysis. RESULTS: Increased SNHG1 expression was found in midbrain of MPTP-induced PD mice and MPP+-treated SH-SY5Y cells. Overexpression of SNHG1 lowered viability and enhanced apoptosis in MPP+-treated SH-SY5Y cells. Moreover, SNHG1 acted as a molecular sponge to inhibit the expression of miR-153-3p. Furthermore, miR-153-3p-mediated suppression of MPP+-induced cytotoxicity was abated following SNHG1 up-regulation. Additionally, PTEN was identified as a direct target of miR-153-3p, and SNHG1 could serve as a competing endogenous RNA (ceRNA) of miR-153-3p to improve the expression of PTEN. Besides, enforced expression of PTEN displayed the similar functions as SNHG1 overexpression in regulating the viability and apoptosis of MPP+-treated SH-SY5Y cells. Finally, SNHG1 was found to activate PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells by targeting miR-153-3p. CONCLUSION: SNHG1 aggravates MPP+-induced cellular toxicity in SH-SY5Y cells by regulating PTEN/AKT/mTOR signaling via sponging miR-153-3p, indicating the potential of SNHG1 as a promising therapeutic target for PD. SN - 0717-6287 UR - https://www.unboundmedicine.com/medline/citation/31907031/SNHG1_promotes_MPP+_induced_cytotoxicity_by_regulating_PTEN/AKT/mTOR_signaling_pathway_in_SH_SY5Y_cells_via_sponging_miR_153_3p_ L2 - https://biolres.biomedcentral.com/articles/10.1186/s40659-019-0267-y DB - PRIME DP - Unbound Medicine ER -