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Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe.
Mikrochim Acta. 2020 01 08; 187(2):104.MA

Abstract

Authors report on a new fluoro-graphene-plasmonic nanohybrid aptamer-based fluorescent nanoprobe for cocaine. To construct the nanoprobe, newly synthesized glutathione-capped ZnS/Ag2Se quantum dots (QDs) were first conjugated to graphene oxide (GO) to form a QD-GO nanocomposite. The binding interaction resulted in a fluorescence turn-ON. Thereafter, cetyltrimethylammonium bromide (CTAB)-gold nanoparticles (AuNPs) were directly adsorbed on the QD-GO nanocomposite to form a novel QD-GO-CTAB-AuNP nanohybrid assembly that resulted in a fluorescence turn-OFF. Streptavidin (strep) was then adsorbed on the QDs-GO-CTAB-AuNP nanohybrid assembly which allowed binding to a biotinylated MNS 4.1 anticocaine DNA aptamer (B) receptor. The addition of cocaine into the strep-B-QDs-GO-CTAB-AuNP aptamer nanoprobe system aided affinity to the aptamer receptor and in turn turned on the fluorescence of the nanoprobe in a concentration-dependent manner. Under optimum experimental conditions, we found the strep-B-QD-GO-CTAB-AuNP to be far superior in its sensitivity to cocaine than the tested strep-B-QDs (no GO and CTAB-AuNPs), strep-B-QD-CTAB-AuNP (no GO) and strep-B-QD-GO (no CTAB-AuNP). In addition, the investigation of localized surface plasmon resonance (LSPR) amplified signal from tested plasmonic NPs shows that CTAB-AuNPs was far superior in amplifying the fluorescence signal of the nanoprobe. A detection limit of 4.6 nM (1.56 ng.mL-1), rapid response time (~2 min) and excellent selectivity against other drugs, substances and cocaine metabolites was achieved. The strep-B-QD-GO-CTAB-AuNP aptamer-based fluorescent nanoprobe was successfully applied for the determination of cocaine in seized adulterated cocaine samples. Graphical abstractSchematic representation of the streptavidin-biotin-quantum dot-graphene oxide-cetyltrimethylammonium bromide-gold nanoparticle aptamer-based fluorescent nanoprobe for cocaine.

Authors+Show Affiliations

Leverhulme Research Centre for Forensic Science, University of Dundee, Dundee, DD1 4GH, United Kingdom. o.adegoke@dundee.ac.uk.Leverhulme Research Centre for Forensic Science, University of Dundee, Dundee, DD1 4GH, United Kingdom.Materials Science & Engineering Research Cluster, School of Science & Engineering, University of Dundee, Dundee, DD1 4HN, United Kingdom.Materials Science & Engineering Research Cluster, School of Science & Engineering, University of Dundee, Dundee, DD1 4HN, United Kingdom.Leverhulme Research Centre for Forensic Science, University of Dundee, Dundee, DD1 4GH, United Kingdom.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31912290

Citation

Adegoke, Oluwasesan, et al. "Aptamer-based Cocaine Assay Using a Nanohybrid Composed of ZnS/Ag2Se Quantum Dots, Graphene Oxide and Gold Nanoparticles as a Fluorescent Probe." Mikrochimica Acta, vol. 187, no. 2, 2020, p. 104.
Adegoke O, Pereira-Barros MA, Zolotovskaya S, et al. Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe. Mikrochim Acta. 2020;187(2):104.
Adegoke, O., Pereira-Barros, M. A., Zolotovskaya, S., Abdolvand, A., & Daeid, N. N. (2020). Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe. Mikrochimica Acta, 187(2), 104. https://doi.org/10.1007/s00604-019-4101-6
Adegoke O, et al. Aptamer-based Cocaine Assay Using a Nanohybrid Composed of ZnS/Ag2Se Quantum Dots, Graphene Oxide and Gold Nanoparticles as a Fluorescent Probe. Mikrochim Acta. 2020 01 8;187(2):104. PubMed PMID: 31912290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe. AU - Adegoke,Oluwasesan, AU - Pereira-Barros,Magda A, AU - Zolotovskaya,Svetlana, AU - Abdolvand,Amin, AU - Daeid,Niamh Nic, Y1 - 2020/01/08/ PY - 2019/09/20/received PY - 2019/12/27/accepted PY - 2020/1/9/entrez PY - 2020/1/9/pubmed PY - 2020/9/10/medline KW - Fluorescence KW - Gold nanoparticle KW - Graphene oxide KW - Nanohybrid KW - Quantum dots SP - 104 EP - 104 JF - Mikrochimica acta JO - Mikrochim Acta VL - 187 IS - 2 N2 - Authors report on a new fluoro-graphene-plasmonic nanohybrid aptamer-based fluorescent nanoprobe for cocaine. To construct the nanoprobe, newly synthesized glutathione-capped ZnS/Ag2Se quantum dots (QDs) were first conjugated to graphene oxide (GO) to form a QD-GO nanocomposite. The binding interaction resulted in a fluorescence turn-ON. Thereafter, cetyltrimethylammonium bromide (CTAB)-gold nanoparticles (AuNPs) were directly adsorbed on the QD-GO nanocomposite to form a novel QD-GO-CTAB-AuNP nanohybrid assembly that resulted in a fluorescence turn-OFF. Streptavidin (strep) was then adsorbed on the QDs-GO-CTAB-AuNP nanohybrid assembly which allowed binding to a biotinylated MNS 4.1 anticocaine DNA aptamer (B) receptor. The addition of cocaine into the strep-B-QDs-GO-CTAB-AuNP aptamer nanoprobe system aided affinity to the aptamer receptor and in turn turned on the fluorescence of the nanoprobe in a concentration-dependent manner. Under optimum experimental conditions, we found the strep-B-QD-GO-CTAB-AuNP to be far superior in its sensitivity to cocaine than the tested strep-B-QDs (no GO and CTAB-AuNPs), strep-B-QD-CTAB-AuNP (no GO) and strep-B-QD-GO (no CTAB-AuNP). In addition, the investigation of localized surface plasmon resonance (LSPR) amplified signal from tested plasmonic NPs shows that CTAB-AuNPs was far superior in amplifying the fluorescence signal of the nanoprobe. A detection limit of 4.6 nM (1.56 ng.mL-1), rapid response time (~2 min) and excellent selectivity against other drugs, substances and cocaine metabolites was achieved. The strep-B-QD-GO-CTAB-AuNP aptamer-based fluorescent nanoprobe was successfully applied for the determination of cocaine in seized adulterated cocaine samples. Graphical abstractSchematic representation of the streptavidin-biotin-quantum dot-graphene oxide-cetyltrimethylammonium bromide-gold nanoparticle aptamer-based fluorescent nanoprobe for cocaine. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/31912290/Aptamer_based_cocaine_assay_using_a_nanohybrid_composed_of_ZnS/Ag2Se_quantum_dots_graphene_oxide_and_gold_nanoparticles_as_a_fluorescent_probe_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31912290/ DB - PRIME DP - Unbound Medicine ER -