Tags

Type your tag names separated by a space and hit enter

Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase.

Abstract

Multiple enzyme systems are being increasingly used for their high-efficiency and co-immobilization is a key technology to lower the cost and improve the stability of enzymes. In this study, poly glycidyl methacrylate (PGMA) spheres were synthesized using suspension polymerization, and were used as a support to co-immobilize glucose oxidase (GOx) and catalase (CAT). Surface modification was carried out via a combination of plasma and amination to promote the properties of the catalyzer. The co-immobilized enzymes showed a more extensive range of optimum pH and temperature from 5.5 to 7.5 and 25 to 40°C, respectively, compared to free enzymes. Furthermore, the maximum activity and protein adsorption quantity of the co-immobilized enzymes reached 25.98 U/g and 6.07 mg/g, respectively. The enzymatic activity of the co-immobilized enzymes was maintained at ~70% after storage for 5 days and at 82% after three consecutive cycles, indicating that the immobilized material could be applied industrially.

Authors+Show Affiliations

School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China. State Key Laboratory of Biobased Material & Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China. State Key Laboratory of Biobased Material & Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31921828

Citation

Liao, Lingtong, et al. "Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase." Frontiers in Bioengineering and Biotechnology, vol. 7, 2019, p. 426.
Liao L, Meng Y, Wang R, et al. Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase. Front Bioeng Biotechnol. 2019;7:426.
Liao, L., Meng, Y., Wang, R., Jia, B., & Li, P. (2019). Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase. Frontiers in Bioengineering and Biotechnology, 7, p. 426. doi:10.3389/fbioe.2019.00426.
Liao L, et al. Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase. Front Bioeng Biotechnol. 2019;7:426. PubMed PMID: 31921828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase. AU - Liao,Lingtong, AU - Meng,Yuling, AU - Wang,Ruiming, AU - Jia,Baolei, AU - Li,Piwu, Y1 - 2019/12/13/ PY - 2019/10/09/received PY - 2019/12/02/accepted PY - 2020/1/11/entrez PY - 2020/1/11/pubmed PY - 2020/1/11/medline KW - catalase KW - co-immobilization KW - glucose oxidase KW - plasma KW - poly glycidyl methacrylate SP - 426 EP - 426 JF - Frontiers in bioengineering and biotechnology JO - Front Bioeng Biotechnol VL - 7 N2 - Multiple enzyme systems are being increasingly used for their high-efficiency and co-immobilization is a key technology to lower the cost and improve the stability of enzymes. In this study, poly glycidyl methacrylate (PGMA) spheres were synthesized using suspension polymerization, and were used as a support to co-immobilize glucose oxidase (GOx) and catalase (CAT). Surface modification was carried out via a combination of plasma and amination to promote the properties of the catalyzer. The co-immobilized enzymes showed a more extensive range of optimum pH and temperature from 5.5 to 7.5 and 25 to 40°C, respectively, compared to free enzymes. Furthermore, the maximum activity and protein adsorption quantity of the co-immobilized enzymes reached 25.98 U/g and 6.07 mg/g, respectively. The enzymatic activity of the co-immobilized enzymes was maintained at ~70% after storage for 5 days and at 82% after three consecutive cycles, indicating that the immobilized material could be applied industrially. SN - 2296-4185 UR - https://www.unboundmedicine.com/medline/citation/31921828/Coupling_and_Regulation_of_Porous_Carriers_Using_Plasma_and_Amination_to_Improve_the_Catalytic_Performance_of_Glucose_Oxidase_and_Catalase L2 - https://doi.org/10.3389/fbioe.2019.00426 DB - PRIME DP - Unbound Medicine ER -