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Immunopathogenesis of canine chronic ulcerative stomatitis.
PLoS One. 2020; 15(1):e0227386.Plos

Abstract

Canine Chronic Ulcerative Stomatitis is a spontaneously occurring inflammatory disease of the oral mucosa. An immune-mediated pathogenesis is suspected though not yet proven. We have recently reported on the clinical and histologic features, and identification of select leukocyte cell populations within the lesion. A clinical and histologic similarity to oral lichen planus of people was proposed. In the present study, these initial observations are extended by examining lesions from 24 dogs with clinical evidence of chronic ulcerative stomatitis. Because dogs with chronic ulcerative stomatitis often have concurrent periodontal disease, we wondered if dental plaque/biofilm may be a common instigator of inflammation in both lesions. We hypothesized that dogs with chronic ulcerative stomatitis would exhibit a spectrum of pathologic changes and phenotype of infiltrating leukocytes that would inform lesion pathogenesis and that these changes would differ from inflammatory phenotypes in periodontitis. Previously we identified chronic ulcerative stomatitis lesions to be rich in FoxP3+ and IL17+ cells. As such, we suspect that these leukocytes play an important role in lesion pathogenesis. The current study confirms the presence of moderate to large numbers of FoxP3+ T cells and IL17+ cells in all ulcerative stomatitis lesions using confocal immunofluorescence. Interestingly, the majority of IL17+ cells were determined to be non-T cells and IL17+ cell frequencies were negatively correlated with severity on the clinical scoring system. Three histologic subtypes of ulcerative stomatitis were determined; lichenoid, deep stomatitis and granulomatous. Periodontitis lesions, like stomatitis lesions, were B cell and plasma cell rich, but otherwise differed from the stomatitis lesions. Direct immunofluorescence results did not support an autoantibody-mediated autoimmune disease process. This investigation contributes to the body of literature regarding leukocyte involvement in canine idiopathic inflammatory disease pathogenesis.

Authors+Show Affiliations

Sacramento Veterinary Dental Services, Rancho Cordova, California, United States of America.Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.Department of Clinical Sciences, College of Veterinary Medicine, NC State University, Raleigh, North Carolina, United States of America.Barrington Animal Hospital, Barrington, Illinois, United States of America.Barrington Animal Hospital, Barrington, Illinois, United States of America.Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31923271

Citation

Anderson, J G., et al. "Immunopathogenesis of Canine Chronic Ulcerative Stomatitis." PloS One, vol. 15, no. 1, 2020, pp. e0227386.
Anderson JG, Kol A, Bizikova P, et al. Immunopathogenesis of canine chronic ulcerative stomatitis. PLoS ONE. 2020;15(1):e0227386.
Anderson, J. G., Kol, A., Bizikova, P., Stapelton, B. P., Ford, K., Villarreal, A., Jimenez, R. J., Vasilatis, D., & Murphy, B. G. (2020). Immunopathogenesis of canine chronic ulcerative stomatitis. PloS One, 15(1), e0227386. https://doi.org/10.1371/journal.pone.0227386
Anderson JG, et al. Immunopathogenesis of Canine Chronic Ulcerative Stomatitis. PLoS ONE. 2020;15(1):e0227386. PubMed PMID: 31923271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunopathogenesis of canine chronic ulcerative stomatitis. AU - Anderson,J G, AU - Kol,A, AU - Bizikova,P, AU - Stapelton,B P, AU - Ford,K, AU - Villarreal,A, AU - Jimenez,R J, AU - Vasilatis,D, AU - Murphy,B G, Y1 - 2020/01/10/ PY - 2019/08/04/received PY - 2019/12/17/accepted PY - 2020/1/11/entrez PY - 2020/1/11/pubmed PY - 2020/4/21/medline SP - e0227386 EP - e0227386 JF - PloS one JO - PLoS ONE VL - 15 IS - 1 N2 - Canine Chronic Ulcerative Stomatitis is a spontaneously occurring inflammatory disease of the oral mucosa. An immune-mediated pathogenesis is suspected though not yet proven. We have recently reported on the clinical and histologic features, and identification of select leukocyte cell populations within the lesion. A clinical and histologic similarity to oral lichen planus of people was proposed. In the present study, these initial observations are extended by examining lesions from 24 dogs with clinical evidence of chronic ulcerative stomatitis. Because dogs with chronic ulcerative stomatitis often have concurrent periodontal disease, we wondered if dental plaque/biofilm may be a common instigator of inflammation in both lesions. We hypothesized that dogs with chronic ulcerative stomatitis would exhibit a spectrum of pathologic changes and phenotype of infiltrating leukocytes that would inform lesion pathogenesis and that these changes would differ from inflammatory phenotypes in periodontitis. Previously we identified chronic ulcerative stomatitis lesions to be rich in FoxP3+ and IL17+ cells. As such, we suspect that these leukocytes play an important role in lesion pathogenesis. The current study confirms the presence of moderate to large numbers of FoxP3+ T cells and IL17+ cells in all ulcerative stomatitis lesions using confocal immunofluorescence. Interestingly, the majority of IL17+ cells were determined to be non-T cells and IL17+ cell frequencies were negatively correlated with severity on the clinical scoring system. Three histologic subtypes of ulcerative stomatitis were determined; lichenoid, deep stomatitis and granulomatous. Periodontitis lesions, like stomatitis lesions, were B cell and plasma cell rich, but otherwise differed from the stomatitis lesions. Direct immunofluorescence results did not support an autoantibody-mediated autoimmune disease process. This investigation contributes to the body of literature regarding leukocyte involvement in canine idiopathic inflammatory disease pathogenesis. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31923271/Immunopathogenesis_of_canine_chronic_ulcerative_stomatitis L2 - http://dx.plos.org/10.1371/journal.pone.0227386 DB - PRIME DP - Unbound Medicine ER -