Tags

Type your tag names separated by a space and hit enter

Development of an analytical method for determination of total ethofumesate residues in foods by gas chromatography-tandem mass spectrometry.
Food Chem. 2020 May 30; 313:126132.FC

Abstract

Analytical method was developed for determining the total residue of ethofumesate (ET) herbicide using GC-MS/MS. The ET residues were analyzed as a sum of ET, 2-keto-ethofumesate (KET), and open-ring-2-keto-ethofumesate (OKET) and its conjugate. The extracted samples were partitioned with hexane and NaOH solution. For ET analysis, the hexane layer was cleaned up by a silica gel cartridge prior to GC-MS/MS analysis. For the analyses of the metabolites, the aqueous layer was heated with HCl to hydrolyze the conjugates, thereafter, heated in acetic anhydride to convert OKET to KET, and cleaned up by a silica gel cartridge prior to GC-MS/MS analysis. The method was validated for ET, KET, and OKET in garlic, onion, and sugar beet at 0.3 and 0.01 mg/kg. The recoveries were 94-113%, with relative standard deviations of <6%. The limits of detection were 0.0005 mg/kg for all analytes. The proposed method is suitable for regulatory analysis.

Authors+Show Affiliations

Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: shizsaito@nihs.go.jp.Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: n-kashiwabara@nihs.go.jp.Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: ks203790@nodai.ac.jp.Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: nemoto@nihs.go.jp.Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: akiyama@nihs.go.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31927207

Citation

Saito-Shida, Shizuka, et al. "Development of an Analytical Method for Determination of Total Ethofumesate Residues in Foods By Gas Chromatography-tandem Mass Spectrometry." Food Chemistry, vol. 313, 2020, p. 126132.
Saito-Shida S, Kashiwabara N, Shiono K, et al. Development of an analytical method for determination of total ethofumesate residues in foods by gas chromatography-tandem mass spectrometry. Food Chem. 2020;313:126132.
Saito-Shida, S., Kashiwabara, N., Shiono, K., Nemoto, S., & Akiyama, H. (2020). Development of an analytical method for determination of total ethofumesate residues in foods by gas chromatography-tandem mass spectrometry. Food Chemistry, 313, 126132. https://doi.org/10.1016/j.foodchem.2019.126132
Saito-Shida S, et al. Development of an Analytical Method for Determination of Total Ethofumesate Residues in Foods By Gas Chromatography-tandem Mass Spectrometry. Food Chem. 2020 May 30;313:126132. PubMed PMID: 31927207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an analytical method for determination of total ethofumesate residues in foods by gas chromatography-tandem mass spectrometry. AU - Saito-Shida,Shizuka, AU - Kashiwabara,Nao, AU - Shiono,Kouji, AU - Nemoto,Satoru, AU - Akiyama,Hiroshi, Y1 - 2019/12/31/ PY - 2019/07/02/received PY - 2019/12/19/revised PY - 2019/12/26/accepted PY - 2020/1/14/pubmed PY - 2020/3/25/medline PY - 2020/1/14/entrez KW - 2-Keto-ethofumesate KW - Ethofumesate KW - GC–MS/MS KW - Hydrolysis KW - Open-ring-2-keto-ethofumesate SP - 126132 EP - 126132 JF - Food chemistry JO - Food Chem VL - 313 N2 - Analytical method was developed for determining the total residue of ethofumesate (ET) herbicide using GC-MS/MS. The ET residues were analyzed as a sum of ET, 2-keto-ethofumesate (KET), and open-ring-2-keto-ethofumesate (OKET) and its conjugate. The extracted samples were partitioned with hexane and NaOH solution. For ET analysis, the hexane layer was cleaned up by a silica gel cartridge prior to GC-MS/MS analysis. For the analyses of the metabolites, the aqueous layer was heated with HCl to hydrolyze the conjugates, thereafter, heated in acetic anhydride to convert OKET to KET, and cleaned up by a silica gel cartridge prior to GC-MS/MS analysis. The method was validated for ET, KET, and OKET in garlic, onion, and sugar beet at 0.3 and 0.01 mg/kg. The recoveries were 94-113%, with relative standard deviations of <6%. The limits of detection were 0.0005 mg/kg for all analytes. The proposed method is suitable for regulatory analysis. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/31927207/Development_of_an_analytical_method_for_determination_of_total_ethofumesate_residues_in_foods_by_gas_chromatography-tandem_mass_spectrometry L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(19)32284-8 DB - PRIME DP - Unbound Medicine ER -