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Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats.
Theriogenology. 2020 Mar 01; 144:98-106.T

Abstract

AIMS

One of the most common urologic emergencies is spermatic cord torsion, which can damage testicular tissue and reduce fertility. Salvia miltiorrhiza (SM) hydroalcoholic extract possess high antioxidant properties, and its efficacy in ischemia-reperfusion (I/R) injury prevention has been demonstrated in cardiac, renal, and liver tissues. Therefore, the purpose of this study was to assess the protective mechanism of SM extract on testicular I/R damage.

MAIN METHODS

18 mature male Wistar albino rats were randomly divided into 3 groups; with six rats in each group: Group 1 (Sham) was sham-operated. Group 2 (T-D): torsion was performed, and after 2 hours (h) detorsion was done. Group 3 (SM): (200 mg kg-1) SM was intraperitoneally injected thirty minutes before detorsion. Then testicular and epididymal weight and size alterations, sperm parameters (motility, livability, concentration, and morphology), both plasma and testicular tissue levels of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), and total antioxidant capacity (TAC) were evaluated. Also, histopathological changes included mean seminiferous tubular diameter (MSTD), testicular capsule thickness (TCT), mean testicular biopsy scoring (MTBS), and germinal epithelial cell thickness (GECT) were examined.

RESULTS

Testicular I/R significantly reduced sperm motility, viability, and normality, while SM extract administration remarkably increased sperm motility, and normality (P < 0.05). Induction of testicular T-D caused a significant increment in the level of MDA and notable decline in the levels of GPX, CAT, and TAC both in plasma and testis tissue, whereas administration of SM extract significantly decreased MDA level and increased GPX, CAT, and TAC levels in plasma and testicular tissue (P < 0.05). Histopathological parameters including MSTD, GECT, MTBS, and TCT were significantly lower in the T-D group, while pretreatment with SM extract remarkably increased MSTD, GECT, and MTBS amounts (P < 0.05).

CONCLUSION

Since the SM extract increased the activity of antioxidant enzymes, improved sperm parameters and reduced the damage to testicular tissue, therefore, its use as a potent antioxidant in reducing testicular I/R damage is suggested.

Authors+Show Affiliations

Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran. Electronic address: raisi.a@lu.ac.ir.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Karaj, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31927420

Citation

Davoodi, Farshid, et al. "Investigating the Sperm Parameters, Oxidative Stress and Histopathological Effects of Salvia Miltiorrhiza Hydroalcoholic Extract in the Prevention of Testicular Ischemia Reperfusion Damage in Rats." Theriogenology, vol. 144, 2020, pp. 98-106.
Davoodi F, Taheri S, Raisi A, et al. Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats. Theriogenology. 2020;144:98-106.
Davoodi, F., Taheri, S., Raisi, A., Rajabzadeh, A., Ahmadvand, H., Hablolvarid, M. H., & Zakian, A. (2020). Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats. Theriogenology, 144, 98-106. https://doi.org/10.1016/j.theriogenology.2020.01.002
Davoodi F, et al. Investigating the Sperm Parameters, Oxidative Stress and Histopathological Effects of Salvia Miltiorrhiza Hydroalcoholic Extract in the Prevention of Testicular Ischemia Reperfusion Damage in Rats. Theriogenology. 2020 Mar 1;144:98-106. PubMed PMID: 31927420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats. AU - Davoodi,Farshid, AU - Taheri,Shayan, AU - Raisi,Abbas, AU - Rajabzadeh,Asghar, AU - Ahmadvand,Hassan, AU - Hablolvarid,Mohammad Hassan, AU - Zakian,Amir, Y1 - 2020/01/03/ PY - 2019/11/01/received PY - 2019/12/22/revised PY - 2020/01/01/accepted PY - 2020/1/14/pubmed PY - 2020/11/25/medline PY - 2020/1/14/entrez KW - Antioxidant KW - Ischemia reperfusion KW - Oxidative stress KW - Salvia miltiorrhiza KW - Sperm parameters KW - Testicular torsion detorsion SP - 98 EP - 106 JF - Theriogenology JO - Theriogenology VL - 144 N2 - AIMS: One of the most common urologic emergencies is spermatic cord torsion, which can damage testicular tissue and reduce fertility. Salvia miltiorrhiza (SM) hydroalcoholic extract possess high antioxidant properties, and its efficacy in ischemia-reperfusion (I/R) injury prevention has been demonstrated in cardiac, renal, and liver tissues. Therefore, the purpose of this study was to assess the protective mechanism of SM extract on testicular I/R damage. MAIN METHODS: 18 mature male Wistar albino rats were randomly divided into 3 groups; with six rats in each group: Group 1 (Sham) was sham-operated. Group 2 (T-D): torsion was performed, and after 2 hours (h) detorsion was done. Group 3 (SM): (200 mg kg-1) SM was intraperitoneally injected thirty minutes before detorsion. Then testicular and epididymal weight and size alterations, sperm parameters (motility, livability, concentration, and morphology), both plasma and testicular tissue levels of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), and total antioxidant capacity (TAC) were evaluated. Also, histopathological changes included mean seminiferous tubular diameter (MSTD), testicular capsule thickness (TCT), mean testicular biopsy scoring (MTBS), and germinal epithelial cell thickness (GECT) were examined. RESULTS: Testicular I/R significantly reduced sperm motility, viability, and normality, while SM extract administration remarkably increased sperm motility, and normality (P < 0.05). Induction of testicular T-D caused a significant increment in the level of MDA and notable decline in the levels of GPX, CAT, and TAC both in plasma and testis tissue, whereas administration of SM extract significantly decreased MDA level and increased GPX, CAT, and TAC levels in plasma and testicular tissue (P < 0.05). Histopathological parameters including MSTD, GECT, MTBS, and TCT were significantly lower in the T-D group, while pretreatment with SM extract remarkably increased MSTD, GECT, and MTBS amounts (P < 0.05). CONCLUSION: Since the SM extract increased the activity of antioxidant enzymes, improved sperm parameters and reduced the damage to testicular tissue, therefore, its use as a potent antioxidant in reducing testicular I/R damage is suggested. SN - 1879-3231 UR - https://www.unboundmedicine.com/medline/citation/31927420/Investigating_the_sperm_parameters_oxidative_stress_and_histopathological_effects_of_salvia_miltiorrhiza_hydroalcoholic_extract_in_the_prevention_of_testicular_ischemia_reperfusion_damage_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0093-691X(20)30002-9 DB - PRIME DP - Unbound Medicine ER -