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Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
Br J Nutr. 2020 Apr 28; 123(8):881-891.BJ

Abstract

The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury. A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge. After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma. Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets. Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin. In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge. These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.

Authors+Show Affiliations

Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China. College of Life Science, South-Central University for Nationalities, Wuhan430074, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China. Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK74078, USA.Department of Animal Science and Nutritional Engineering, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan430023, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31928547

Citation

Xu, Xiao, et al. "Holly Polyphenols Alleviate Intestinal Inflammation and Alter Microbiota Composition in Lipopolysaccharide-challenged Pigs." The British Journal of Nutrition, vol. 123, no. 8, 2020, pp. 881-891.
Xu X, Hua H, Wang L, et al. Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs. Br J Nutr. 2020;123(8):881-891.
Xu, X., Hua, H., Wang, L., He, P., Zhang, L., Qin, Q., Yu, C., Wang, X., Zhang, G., & Liu, Y. (2020). Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs. The British Journal of Nutrition, 123(8), 881-891. https://doi.org/10.1017/S0007114520000082
Xu X, et al. Holly Polyphenols Alleviate Intestinal Inflammation and Alter Microbiota Composition in Lipopolysaccharide-challenged Pigs. Br J Nutr. 2020 Apr 28;123(8):881-891. PubMed PMID: 31928547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs. AU - Xu,Xiao, AU - Hua,Hongwei, AU - Wang,Longmei, AU - He,Pengwei, AU - Zhang,Lin, AU - Qin,Qin, AU - Yu,Cheng, AU - Wang,Xiuying, AU - Zhang,Guolong, AU - Liu,Yulan, Y1 - 2020/01/13/ PY - 2020/1/14/pubmed PY - 2020/1/14/medline PY - 2020/1/14/entrez KW - Inflammation KW - Intestine KW - Lipopolysaccharide KW - Piglets KW - Polyphenols SP - 881 EP - 891 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 123 IS - 8 N2 - The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury. A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge. After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma. Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets. Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin. In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge. These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/31928547/Holly_polyphenols_alleviate_intestinal_inflammation_and_alter_microbiota_composition_in_lipopolysaccharide-challenged_pigs L2 - https://www.cambridge.org/core/product/identifier/S0007114520000082/type/journal_article DB - PRIME DP - Unbound Medicine ER -
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