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Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair.
PLoS One 2020; 15(1):e0227563Plos

Abstract

Rotator cuff tear is the most frequent tendon injury in the adult population. Despite current improvements in surgical techniques and the development of grafts, failure rates following tendon reconstruction remain high. New therapies, which aim to restore the topology and functionality of the interface between muscle, tendon and bone, are essentially required. One of the key factors for a successful incorporation of tissue engineered constructs is a rapid ingrowth of cells and tissues, which is dependent on a fast vascularization. The dorsal skinfold chamber model in female BALB/cJZtm mice allows the observation of microhemodynamic parameters in repeated measurements in vivo and therefore the description of the vascularization of different implant materials. In order to promote vascularization of implant material, we compared a porous polymer patch (a commercially available porous polyurethane based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fiber mats and chitosan-graft-PCL coated electrospun PCL (CS-g-PCL) fiber mats in vivo. Using intravital fluorescence microscopy microcirculatory parameters were analyzed repetitively over 14 days. Vascularization was significantly increased in CS-g-PCL fiber mats at day 14 compared to the porous polymer patch and uncoated PCL fiber mats. Furthermore CS-g-PCL fiber mats showed also a reduced activation of immune cells. Clinically, these are important findings as they indicate that the CS-g-PCL improves the formation of vascularized tissue and the ingrowth of cells into electrospun PCL scaffolds. Especially the combination of enhanced vascularization and the reduction in immune cell activation at the later time points of our study points to an improved clinical outcome after rotator cuff tear repair.

Authors+Show Affiliations

Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany. NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany.Institute for Anatomy, University of Veterinary Medicine Hannover, Hannover, Germany.NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany. Department of Orthopedic Surgery, Laboratory for Biomechanics and Biomaterials, Graded Implants and Regenerative Strategies, Hannover Medical School, Hannover, Germany.Institute for Technical Chemistry, Braunschweig University of Technology, Braunschweig, Germany.Institute for Technical Chemistry, Braunschweig University of Technology, Braunschweig, Germany.NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany. Institute of Multiphase Processes, Leibniz University Hannover, Hannover, Germany.NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany. Institute of Multiphase Processes, Leibniz University Hannover, Hannover, Germany.NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany. Department of Orthopedic Surgery, Hannover Medical School, Hannover, Germany.NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany. Department of Orthopedic Surgery, Hannover Medical School, Hannover, Germany.Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States of America.Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany. NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31929570

Citation

Gniesmer, Sarah, et al. "Vascularization and Biocompatibility of Poly(ε-caprolactone) Fiber Mats for Rotator Cuff Tear Repair." PloS One, vol. 15, no. 1, 2020, pp. e0227563.
Gniesmer S, Brehm R, Hoffmann A, et al. Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair. PLoS ONE. 2020;15(1):e0227563.
Gniesmer, S., Brehm, R., Hoffmann, A., de Cassan, D., Menzel, H., Hoheisel, A. L., ... Kampmann, A. (2020). Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair. PloS One, 15(1), pp. e0227563. doi:10.1371/journal.pone.0227563.
Gniesmer S, et al. Vascularization and Biocompatibility of Poly(ε-caprolactone) Fiber Mats for Rotator Cuff Tear Repair. PLoS ONE. 2020;15(1):e0227563. PubMed PMID: 31929570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair. AU - Gniesmer,Sarah, AU - Brehm,Ralph, AU - Hoffmann,Andrea, AU - de Cassan,Dominik, AU - Menzel,Henning, AU - Hoheisel,Anna Lena, AU - Glasmacher,Birgit, AU - Willbold,Elmar, AU - Reifenrath,Janin, AU - Ludwig,Nils, AU - Zimmerer,Ruediger, AU - Tavassol,Frank, AU - Gellrich,Nils-Claudius, AU - Kampmann,Andreas, Y1 - 2020/01/13/ PY - 2019/08/13/received PY - 2019/12/20/accepted PY - 2020/1/14/entrez PY - 2020/1/14/pubmed PY - 2020/1/14/medline SP - e0227563 EP - e0227563 JF - PloS one JO - PLoS ONE VL - 15 IS - 1 N2 - Rotator cuff tear is the most frequent tendon injury in the adult population. Despite current improvements in surgical techniques and the development of grafts, failure rates following tendon reconstruction remain high. New therapies, which aim to restore the topology and functionality of the interface between muscle, tendon and bone, are essentially required. One of the key factors for a successful incorporation of tissue engineered constructs is a rapid ingrowth of cells and tissues, which is dependent on a fast vascularization. The dorsal skinfold chamber model in female BALB/cJZtm mice allows the observation of microhemodynamic parameters in repeated measurements in vivo and therefore the description of the vascularization of different implant materials. In order to promote vascularization of implant material, we compared a porous polymer patch (a commercially available porous polyurethane based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fiber mats and chitosan-graft-PCL coated electrospun PCL (CS-g-PCL) fiber mats in vivo. Using intravital fluorescence microscopy microcirculatory parameters were analyzed repetitively over 14 days. Vascularization was significantly increased in CS-g-PCL fiber mats at day 14 compared to the porous polymer patch and uncoated PCL fiber mats. Furthermore CS-g-PCL fiber mats showed also a reduced activation of immune cells. Clinically, these are important findings as they indicate that the CS-g-PCL improves the formation of vascularized tissue and the ingrowth of cells into electrospun PCL scaffolds. Especially the combination of enhanced vascularization and the reduction in immune cell activation at the later time points of our study points to an improved clinical outcome after rotator cuff tear repair. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31929570/Vascularization_and_biocompatibility_of_poly(ε-caprolactone)_fiber_mats_for_rotator_cuff_tear_repair L2 - http://dx.plos.org/10.1371/journal.pone.0227563 DB - PRIME DP - Unbound Medicine ER -