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Erythropoietin Alleviates Burn-induced Muscle Wasting.
Int J Med Sci 2020; 17(1):33-44IJ

Abstract

Background:

Burn injury induces long-term skeletal muscle pathology. We hypothesized EPO could attenuate burn-induced muscle fiber atrophy.

Methods:

Rats were allocated into four groups: a sham burn group, an untreated burn group subjected to third degree hind paw burn, and two burn groups treated with weekly or daily EPO for four weeks. Gastrocnemius muscle was analyzed at four weeks post-burn.

Results:

EPO attenuated the reduction of mean myofiber cross-sectional area post-burn and the level of the protective effect was no significant difference between two EPO-treated groups (p=0.784). Furthermore, EPO decreased the expression of atrophy-related ubiquitin ligase, atrogin-1, which was up-regulated in response to burn. Compared to untreated burn rats, those receiving weekly or daily EPO groups had less cell apoptosis by TUNEL assay. EPO decreased the expression of cleaved caspase 3 (key factor in the caspase-dependent pathway) and apoptosis-inducing factor (implicated in the caspase-independent pathway) after burn. Furthermore, EPO alleviated connective tissue overproduction following burn via transforming growth factor beta 1-Smad2/3 pathway. Daily EPO group caused significant erythrocytosis compared with untreated burn group but not weekly EPO group.

Conclusion:

EPO therapy attenuated skeletal muscle apoptosis and fibrosis at four weeks post-burn. Weekly EPO may be a safe and effective option in muscle wasting post-burn.

Authors+Show Affiliations

Department of Anesthesiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Anesthesiology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Anesthesiology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Anesthesiology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan.Department of Anesthesiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Anesthesiology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Anesthesiology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.Center for Neuroscience, National Sun Yat-Sen University, Kaohsiung, Taiwan. Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.Departments of Pathology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Surgery, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.Department of Surgery, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Regeneration Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31929736

Citation

Wu, Sheng-Hua, et al. "Erythropoietin Alleviates Burn-induced Muscle Wasting." International Journal of Medical Sciences, vol. 17, no. 1, 2020, pp. 33-44.
Wu SH, Lu IC, Tai MH, et al. Erythropoietin Alleviates Burn-induced Muscle Wasting. Int J Med Sci. 2020;17(1):33-44.
Wu, S. H., Lu, I. C., Tai, M. H., Chai, C. Y., Kwan, A. L., & Huang, S. H. (2020). Erythropoietin Alleviates Burn-induced Muscle Wasting. International Journal of Medical Sciences, 17(1), pp. 33-44. doi:10.7150/ijms.38590.
Wu SH, et al. Erythropoietin Alleviates Burn-induced Muscle Wasting. Int J Med Sci. 2020;17(1):33-44. PubMed PMID: 31929736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Erythropoietin Alleviates Burn-induced Muscle Wasting. AU - Wu,Sheng-Hua, AU - Lu,I-Cheng, AU - Tai,Ming-Hong, AU - Chai,Chee-Yin, AU - Kwan,Aij-Lie, AU - Huang,Shu-Hung, Y1 - 2020/01/01/ PY - 2019/07/20/received PY - 2019/11/05/accepted PY - 2020/1/14/entrez PY - 2020/1/14/pubmed PY - 2020/1/14/medline KW - Apoptosis Inducing Factor KW - Burn injury KW - Erythropoietin KW - Muscle fiber atrophy KW - Transforming Growth Factor beta1 SP - 33 EP - 44 JF - International journal of medical sciences JO - Int J Med Sci VL - 17 IS - 1 N2 - Background: Burn injury induces long-term skeletal muscle pathology. We hypothesized EPO could attenuate burn-induced muscle fiber atrophy. Methods: Rats were allocated into four groups: a sham burn group, an untreated burn group subjected to third degree hind paw burn, and two burn groups treated with weekly or daily EPO for four weeks. Gastrocnemius muscle was analyzed at four weeks post-burn. Results: EPO attenuated the reduction of mean myofiber cross-sectional area post-burn and the level of the protective effect was no significant difference between two EPO-treated groups (p=0.784). Furthermore, EPO decreased the expression of atrophy-related ubiquitin ligase, atrogin-1, which was up-regulated in response to burn. Compared to untreated burn rats, those receiving weekly or daily EPO groups had less cell apoptosis by TUNEL assay. EPO decreased the expression of cleaved caspase 3 (key factor in the caspase-dependent pathway) and apoptosis-inducing factor (implicated in the caspase-independent pathway) after burn. Furthermore, EPO alleviated connective tissue overproduction following burn via transforming growth factor beta 1-Smad2/3 pathway. Daily EPO group caused significant erythrocytosis compared with untreated burn group but not weekly EPO group. Conclusion: EPO therapy attenuated skeletal muscle apoptosis and fibrosis at four weeks post-burn. Weekly EPO may be a safe and effective option in muscle wasting post-burn. SN - 1449-1907 UR - https://www.unboundmedicine.com/medline/citation/31929736/Erythropoietin_Alleviates_Burn-induced_Muscle_Wasting L2 - http://www.medsci.org/v17p0033.htm DB - PRIME DP - Unbound Medicine ER -