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A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew.
Theor Appl Genet. 2020 Apr; 133(4):1149-1159.TA

Abstract

KEY MESSAGE

A spontaneous Robertsonian T4SlS·4BL translocation chromosome carrying Pm66 for powdery mildew resistance was discovered and confirmed by RNA-seq, molecular marker, and in situ hybridization analyses. Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a severe disease of bread wheat worldwide. Discovery and utilization of resistance genes to powdery mildew from wild relatives of wheat have played important roles in wheat improvement. Aegilops longissima, one of the S-genome diploid wild relatives of wheat, is a valuable source of disease and pest resistance for wheat. Chromosome 4Sl from Ae. longissima confers moderate resistance to powdery mildew. In this study, we conducted RNA-seq on a putative Chinese Spring (CS)-Ae. longissima 4Sl(4B) disomic substitution line (TA3465) to develop 4Sl-specific markers to assist the transfer of a Bgt resistance gene from 4Sl by induced homoeologous recombination. A pairwise comparison of genes between CS and TA3465 demonstrated that a number of genes on chromosome 4BS in CS were not expressed in TA3465. Analysis of 4B- and 4Sl-specific molecular markers showed that 4BS and 4SlL were both missing in TA3465, whereas 4BL and 4SlS were present. Further characterization by genomic and fluorescent in situ hybridization confirmed that TA3465 carried a spontaneous Robertsonian T4SlS·4BL translocation. Powdery mildew tests showed that TA3465 was resistant to 10 of 16 Bgt isolates collected from different regions of China, whereas CS was susceptible to all those Bgt isolates. The powdery mildew resistance gene(s) in TA3465 was further mapped to the short arm of 4Sl and designated as Pm66.

Authors+Show Affiliations

College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, 57006, USA.Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.College of Life Sciences, Yantai University, Yantai, 264005, People's Republic of China. ptma@ytu.edu.cn.College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. wxliu2003@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31932954

Citation

Li, Huanhuan, et al. "A Spontaneous wheat-Aegilops Longissima Translocation Carrying Pm66 Confers Resistance to Powdery Mildew." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 4, 2020, pp. 1149-1159.
Li H, Dong Z, Ma C, et al. A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew. Theor Appl Genet. 2020;133(4):1149-1159.
Li, H., Dong, Z., Ma, C., Xia, Q., Tian, X., Sehgal, S., Koo, D. H., Friebe, B., Ma, P., & Liu, W. (2020). A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(4), 1149-1159. https://doi.org/10.1007/s00122-020-03538-8
Li H, et al. A Spontaneous wheat-Aegilops Longissima Translocation Carrying Pm66 Confers Resistance to Powdery Mildew. Theor Appl Genet. 2020;133(4):1149-1159. PubMed PMID: 31932954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew. AU - Li,Huanhuan, AU - Dong,Zhenjie, AU - Ma,Chao, AU - Xia,Qing, AU - Tian,Xiubin, AU - Sehgal,Sunish, AU - Koo,Dal-Hoe, AU - Friebe,Bernd, AU - Ma,Pengtao, AU - Liu,Wenxuan, Y1 - 2020/01/13/ PY - 2019/06/22/received PY - 2020/01/03/accepted PY - 2020/1/15/pubmed PY - 2020/11/11/medline PY - 2020/1/15/entrez SP - 1149 EP - 1159 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 4 N2 - KEY MESSAGE: A spontaneous Robertsonian T4SlS·4BL translocation chromosome carrying Pm66 for powdery mildew resistance was discovered and confirmed by RNA-seq, molecular marker, and in situ hybridization analyses. Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a severe disease of bread wheat worldwide. Discovery and utilization of resistance genes to powdery mildew from wild relatives of wheat have played important roles in wheat improvement. Aegilops longissima, one of the S-genome diploid wild relatives of wheat, is a valuable source of disease and pest resistance for wheat. Chromosome 4Sl from Ae. longissima confers moderate resistance to powdery mildew. In this study, we conducted RNA-seq on a putative Chinese Spring (CS)-Ae. longissima 4Sl(4B) disomic substitution line (TA3465) to develop 4Sl-specific markers to assist the transfer of a Bgt resistance gene from 4Sl by induced homoeologous recombination. A pairwise comparison of genes between CS and TA3465 demonstrated that a number of genes on chromosome 4BS in CS were not expressed in TA3465. Analysis of 4B- and 4Sl-specific molecular markers showed that 4BS and 4SlL were both missing in TA3465, whereas 4BL and 4SlS were present. Further characterization by genomic and fluorescent in situ hybridization confirmed that TA3465 carried a spontaneous Robertsonian T4SlS·4BL translocation. Powdery mildew tests showed that TA3465 was resistant to 10 of 16 Bgt isolates collected from different regions of China, whereas CS was susceptible to all those Bgt isolates. The powdery mildew resistance gene(s) in TA3465 was further mapped to the short arm of 4Sl and designated as Pm66. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31932954/A_spontaneous_wheat_Aegilops_longissima_translocation_carrying_Pm66_confers_resistance_to_powdery_mildew_ L2 - https://dx.doi.org/10.1007/s00122-020-03538-8 DB - PRIME DP - Unbound Medicine ER -