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A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal.
Int J Microbiol 2019; 2019:6754058IJ

Abstract

Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal. A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis. Mycological identification revealed Trichophyton soudanense (n = 13), T. interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T. rubrum, T. mentagrophytes, and M. canis and one unidentified strain. For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. ITS sequences showed, in BLAST search analysis, 99-100% of similarity. Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases. Discrepancies concern mostly T. rubrum misidentified as T. interdigitale. PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics. It was also able to give correct identification of an atypical strain of M. audouinii responsible of mycetoma of the scalp.

Authors+Show Affiliations

Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal. Service de Parasitologie-Mycologie, Faculté de Médecine, de Pharmacie et d'Odontologie, Université Cheikh Anta Diop, BP 16477, Dakar, Senegal.Institut Hospitalo-Universitaire Méditerranée Infection, 13 005 Marseille, France.Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal.Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal. Service de Parasitologie-Mycologie, Faculté de Médecine, de Pharmacie et d'Odontologie, Université Cheikh Anta Diop, BP 16477, Dakar, Senegal.Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal. Service de Parasitologie-Mycologie, Faculté de Médecine, de Pharmacie et d'Odontologie, Université Cheikh Anta Diop, BP 16477, Dakar, Senegal.Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal. Service de Parasitologie-Mycologie, Faculté de Médecine, de Pharmacie et d'Odontologie, Université Cheikh Anta Diop, BP 16477, Dakar, Senegal.Institut Hospitalo-Universitaire Méditerranée Infection, 13 005 Marseille, France.Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, Senegal. Service de Parasitologie-Mycologie, Faculté de Médecine, de Pharmacie et d'Odontologie, Université Cheikh Anta Diop, BP 16477, Dakar, Senegal.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31933647

Citation

Diongue, Khadim, et al. "A Comparative Study On Phenotypic Versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal." International Journal of Microbiology, vol. 2019, 2019, p. 6754058.
Diongue K, Bréchard L, Diallo MA, et al. A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. Int J Microbiol. 2019;2019:6754058.
Diongue, K., Bréchard, L., Diallo, M. A., Seck, M. C., Ndiaye, M., Badiane, A. S., ... Ndiaye, D. (2019). A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. International Journal of Microbiology, 2019, p. 6754058. doi:10.1155/2019/6754058.
Diongue K, et al. A Comparative Study On Phenotypic Versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. Int J Microbiol. 2019;2019:6754058. PubMed PMID: 31933647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. AU - Diongue,Khadim, AU - Bréchard,Ludivine, AU - Diallo,Mamadou Alpha, AU - Seck,Mame Cheikh, AU - Ndiaye,Mouhamadou, AU - Badiane,Aida Sadikh, AU - Ranque,Stéphane, AU - Ndiaye,Daouda, Y1 - 2019/12/18/ PY - 2019/08/16/received PY - 2019/11/05/revised PY - 2019/11/27/accepted PY - 2020/1/15/entrez PY - 2020/1/15/pubmed PY - 2020/1/15/medline SP - 6754058 EP - 6754058 JF - International journal of microbiology JO - Int J Microbiol VL - 2019 N2 - Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal. A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis. Mycological identification revealed Trichophyton soudanense (n = 13), T. interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T. rubrum, T. mentagrophytes, and M. canis and one unidentified strain. For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. ITS sequences showed, in BLAST search analysis, 99-100% of similarity. Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases. Discrepancies concern mostly T. rubrum misidentified as T. interdigitale. PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics. It was also able to give correct identification of an atypical strain of M. audouinii responsible of mycetoma of the scalp. SN - 1687-918X UR - https://www.unboundmedicine.com/medline/citation/31933647/A_Comparative_Study_on_Phenotypic_versus_ITS-Based_Molecular_Identification_of_Dermatophytes_Isolated_in_Dakar,_Senegal L2 - https://dx.doi.org/10.1155/2019/6754058 DB - PRIME DP - Unbound Medicine ER -