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Down-regulation of LncRNA UCA1 alleviates liver injury in rats with liver cirrhosis.
Int J Clin Exp Pathol 2019; 12(2):455-465IJ

Abstract

OBJECTIVE

The purpose of this study is to explore the role of long non-coding RNA HULC (lncRNA HULC) in liver injury of rats with cirrhosis.

METHODS

The rat model of liver cirrhosis was induced by dimethylnitrosamine (DMN), which was intraperitoneally injected with siRNA-negative control (NC) or HULC siRNA. HULC expression in rat liver tissues was detected by qRT-PCR. The amounts of alanine transaminase (ALT) and aspartate aminotransferase (AST) in serum and levels of malonyldialdehyde (MDA) and superoxide dismutase (SOD) in liver tissues were measured. TUNEL staining was used to determine hepatocyte apoptosis. Western blot analysis was used to detect the expression of Caspase-3, Bax, and Bcl-2 in liver tissues. qRT-PCR and ELISA were used to detect the mRNA levels and contents of IL-1β and TNF-α in liver tissues and serum of rats, respectively.

RESULTS

High expression of HULC was found in liver tissues of rats with liver cirrhosis. Downregulation of HULC reduced the contents of ALT and AST in serum of rats, inhibited liver tissue lesions and liver fibrosis in rats, suppressed apoptosis (lower expression of caspase-3 and Bax as well as higher BCL-2 expression) of hepatocytes in rats, and inhibited oxidative stress (decreased MDA and increased SOD) and inflammatory injury (decreased IL-1β and TNF-α) in rats with cirrhosis.

CONCLUSION

The findings in this study highlight that the expression of HULC is up-regulated in liver tissues of rats with cirrhosis, and down-regulation of UCA1 could inhibit liver injury in rats with cirrhosis.

Authors+Show Affiliations

Department of Infectious Diseases, Tongde Hospital of Zhejiang Province Hangzhou, PR China.Department of Infectious Diseases, Tongde Hospital of Zhejiang Province Hangzhou, PR China.Department of Infectious Diseases, Tongde Hospital of Zhejiang Province Hangzhou, PR China.Department of Infectious Diseases, Tongde Hospital of Zhejiang Province Hangzhou, PR China.Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat-sen University Zhuhai, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31933850

Citation

Zhu, Yinhong, et al. "Down-regulation of LncRNA UCA1 Alleviates Liver Injury in Rats With Liver Cirrhosis." International Journal of Clinical and Experimental Pathology, vol. 12, no. 2, 2019, pp. 455-465.
Zhu Y, Chen X, Zheng C, et al. Down-regulation of LncRNA UCA1 alleviates liver injury in rats with liver cirrhosis. Int J Clin Exp Pathol. 2019;12(2):455-465.
Zhu, Y., Chen, X., Zheng, C., Rao, X., & Peng, X. (2019). Down-regulation of LncRNA UCA1 alleviates liver injury in rats with liver cirrhosis. International Journal of Clinical and Experimental Pathology, 12(2), pp. 455-465.
Zhu Y, et al. Down-regulation of LncRNA UCA1 Alleviates Liver Injury in Rats With Liver Cirrhosis. Int J Clin Exp Pathol. 2019;12(2):455-465. PubMed PMID: 31933850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulation of LncRNA UCA1 alleviates liver injury in rats with liver cirrhosis. AU - Zhu,Yinhong, AU - Chen,Xiaobei, AU - Zheng,Chunhua, AU - Rao,Xianlin, AU - Peng,Xiaomou, Y1 - 2019/02/01/ PY - 2018/09/05/received PY - 2018/12/06/accepted PY - 2020/1/15/entrez PY - 2020/1/15/pubmed PY - 2020/1/15/medline KW - Liver cirrhosis KW - UCA1 KW - hepatocytes apoptosis KW - inflammatory injury KW - liver injury KW - oxidative stress SP - 455 EP - 465 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 12 IS - 2 N2 - OBJECTIVE: The purpose of this study is to explore the role of long non-coding RNA HULC (lncRNA HULC) in liver injury of rats with cirrhosis. METHODS: The rat model of liver cirrhosis was induced by dimethylnitrosamine (DMN), which was intraperitoneally injected with siRNA-negative control (NC) or HULC siRNA. HULC expression in rat liver tissues was detected by qRT-PCR. The amounts of alanine transaminase (ALT) and aspartate aminotransferase (AST) in serum and levels of malonyldialdehyde (MDA) and superoxide dismutase (SOD) in liver tissues were measured. TUNEL staining was used to determine hepatocyte apoptosis. Western blot analysis was used to detect the expression of Caspase-3, Bax, and Bcl-2 in liver tissues. qRT-PCR and ELISA were used to detect the mRNA levels and contents of IL-1β and TNF-α in liver tissues and serum of rats, respectively. RESULTS: High expression of HULC was found in liver tissues of rats with liver cirrhosis. Downregulation of HULC reduced the contents of ALT and AST in serum of rats, inhibited liver tissue lesions and liver fibrosis in rats, suppressed apoptosis (lower expression of caspase-3 and Bax as well as higher BCL-2 expression) of hepatocytes in rats, and inhibited oxidative stress (decreased MDA and increased SOD) and inflammatory injury (decreased IL-1β and TNF-α) in rats with cirrhosis. CONCLUSION: The findings in this study highlight that the expression of HULC is up-regulated in liver tissues of rats with cirrhosis, and down-regulation of UCA1 could inhibit liver injury in rats with cirrhosis. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/31933850/Down_regulation_of_LncRNA_UCA1_alleviates_liver_injury_in_rats_with_liver_cirrhosis_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31933850/ DB - PRIME DP - Unbound Medicine ER -