Tags

Type your tag names separated by a space and hit enter

Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China.
Parasit Vectors 2020; 13(1):28PV

Abstract

BACKGROUND

Aedes albopictus is naturally infected with Wolbachia spp., maternally transmitted bacteria that influence the reproduction of hosts. However, little is known regarding the prevalence of infection, multiple infection status, and the relationship between Wolbachia density and dengue outbreaks in different regions. Here, we assessed Wolbachia infection in natural populations of Ae. albopictus in China and compared Wolbachia density between regions with similar climates, without dengue and with either imported or local dengue.

RESULTS

To explore the prevalence of Wolbachia infection, Wolbachia DNA was detected in mosquito samples via PCR amplification of the 16S rRNA gene and the surface protein gene wsp. We found that 93.36% of Ae. albopictus in China were positive for Wolbachia. After sequencing gatB, coxA, hcpA, ftsZ, fbpA and wsp genes of Wolbachia strains, we identified a new sequence type (ST) of wAlbB (464/465). Phylogenetic analysis indicated that wAlbA and wAlbB strains formed a cluster with strains from other mosquitoes in a wsp-based maximum likelihood (ML) tree. However, in a ML tree based on multilocus sequence typing (MLST), wAlbB STs (464/465) did not form a cluster with Wolbachia strains from other mosquitoes. To better understand the association between Wolbachia spp. and dengue infection, the prevalence of Wolbachia in Ae. albopictus from different regions (containing local dengue cases, imported dengue cases and no dengue cases) was determined. We found that the prevalence of Wolbachia was lower in regions with only imported dengue cases.

CONCLUSIONS

The natural prevalence of Wolbachia infections in China was much lower than in other countries or regions. The phylogenetic relationships among Wolbachia spp. isolated from field-collected Ae. albopictus reflected the presence of dominant and stable strains. However, wAlbB (464/465) and Wolbachia strains did not form a clade with Wolbachia strains from other mosquitoes. Moreover, lower densities of Wolbachia in regions with only imported dengue cases suggest a relationship between fluctuations in Wolbachia density in field-collected Ae. albopictus and the potential for dengue invasion into these regions.

Authors+Show Affiliations

National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China. huyap9009@163.com. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, China. huyap9009@163.com. Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China. huyap9009@163.com.Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University-Michigan State University Joint Center of Vector Control for Tropical Diseases, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China.Haikou Center for Disease Control and Prevention, Haikou, China.Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China. c_bin@hotmail.com.National Institute for Communicable Disease Control and Prevention, CDC China, Beijing, China. liuqiyong@icdc.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31937373

Citation

Hu, Yaping, et al. "Identification and Molecular Characterization of Wolbachia Strains in Natural Populations of Aedes Albopictus in China." Parasites & Vectors, vol. 13, no. 1, 2020, p. 28.
Hu Y, Xi Z, Liu X, et al. Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China. Parasit Vectors. 2020;13(1):28.
Hu, Y., Xi, Z., Liu, X., Wang, J., Guo, Y., Ren, D., ... Liu, Q. (2020). Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China. Parasites & Vectors, 13(1), p. 28. doi:10.1186/s13071-020-3899-4.
Hu Y, et al. Identification and Molecular Characterization of Wolbachia Strains in Natural Populations of Aedes Albopictus in China. Parasit Vectors. 2020 Jan 14;13(1):28. PubMed PMID: 31937373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China. AU - Hu,Yaping, AU - Xi,Zhiyong, AU - Liu,Xiaobo, AU - Wang,Jun, AU - Guo,Yuhong, AU - Ren,Dongsheng, AU - Wu,Haixia, AU - Wang,Xiaohua, AU - Chen,Bin, AU - Liu,Qiyong, Y1 - 2020/01/14/ PY - 2019/05/11/received PY - 2020/01/07/accepted PY - 2020/1/16/entrez PY - 2020/1/16/pubmed PY - 2020/1/16/medline KW - Aedes albopictus KW - Dengue virus KW - Infection KW - MLST genes KW - Phylogenetic analysis KW - Wolbachia SP - 28 EP - 28 JF - Parasites & vectors JO - Parasit Vectors VL - 13 IS - 1 N2 - BACKGROUND: Aedes albopictus is naturally infected with Wolbachia spp., maternally transmitted bacteria that influence the reproduction of hosts. However, little is known regarding the prevalence of infection, multiple infection status, and the relationship between Wolbachia density and dengue outbreaks in different regions. Here, we assessed Wolbachia infection in natural populations of Ae. albopictus in China and compared Wolbachia density between regions with similar climates, without dengue and with either imported or local dengue. RESULTS: To explore the prevalence of Wolbachia infection, Wolbachia DNA was detected in mosquito samples via PCR amplification of the 16S rRNA gene and the surface protein gene wsp. We found that 93.36% of Ae. albopictus in China were positive for Wolbachia. After sequencing gatB, coxA, hcpA, ftsZ, fbpA and wsp genes of Wolbachia strains, we identified a new sequence type (ST) of wAlbB (464/465). Phylogenetic analysis indicated that wAlbA and wAlbB strains formed a cluster with strains from other mosquitoes in a wsp-based maximum likelihood (ML) tree. However, in a ML tree based on multilocus sequence typing (MLST), wAlbB STs (464/465) did not form a cluster with Wolbachia strains from other mosquitoes. To better understand the association between Wolbachia spp. and dengue infection, the prevalence of Wolbachia in Ae. albopictus from different regions (containing local dengue cases, imported dengue cases and no dengue cases) was determined. We found that the prevalence of Wolbachia was lower in regions with only imported dengue cases. CONCLUSIONS: The natural prevalence of Wolbachia infections in China was much lower than in other countries or regions. The phylogenetic relationships among Wolbachia spp. isolated from field-collected Ae. albopictus reflected the presence of dominant and stable strains. However, wAlbB (464/465) and Wolbachia strains did not form a clade with Wolbachia strains from other mosquitoes. Moreover, lower densities of Wolbachia in regions with only imported dengue cases suggest a relationship between fluctuations in Wolbachia density in field-collected Ae. albopictus and the potential for dengue invasion into these regions. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/31937373/Identification_and_molecular_characterization_of_Wolbachia_strains_in_natural_populations_of_Aedes_albopictus_in_China L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-3899-4 DB - PRIME DP - Unbound Medicine ER -