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Single-cell transcriptional profiles in human skeletal muscle.
Sci Rep 2020; 10(1):229SR

Abstract

Skeletal muscle is a heterogeneous tissue comprised of muscle fiber and mononuclear cell types that, in addition to movement, influences immunity, metabolism and cognition. We investigated the gene expression patterns of skeletal muscle cells using RNA-seq of subtype-pooled single human muscle fibers and single cell RNA-seq of mononuclear cells from human vastus lateralis, mouse quadriceps, and mouse diaphragm. We identified 11 human skeletal muscle mononuclear cell types, including two fibro-adipogenic progenitor (FAP) cell subtypes. The human FBN1+ FAP cell subtype is novel and a corresponding FBN1+ FAP cell type was also found in single cell RNA-seq analysis in mouse. Transcriptome exercise studies using bulk tissue analysis do not resolve changes in individual cell-type proportion or gene expression. The cell-type gene signatures provide the means to use computational methods to identify cell-type level changes in bulk studies. As an example, we analyzed public transcriptome data from an exercise training study and revealed significant changes in specific mononuclear cell-type proportions related to age, sex, acute exercise and training. Our single-cell expression map of skeletal muscle cell types will further the understanding of the diverse effects of exercise and the pathophysiology of muscle disease.

Authors+Show Affiliations

Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA.Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA.Human Performance Laboratory, Ball State University, Muncie, Indiana, 47306, USA.Human Performance Laboratory, Ball State University, Muncie, Indiana, 47306, USA.Human Performance Laboratory, Ball State University, Muncie, Indiana, 47306, USA.Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA.Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA.Department of Neurology, Boston University Medical Center, Boston, MA, 02118, USA.Department of Neurology, Boston University Medical Center, Boston, MA, 02118, USA.Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA.Human Performance Laboratory, Ball State University, Muncie, Indiana, 47306, USA.Human Performance Laboratory, Ball State University, Muncie, Indiana, 47306, USA.Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. stuart.sealfon@mssm.edu. Center for Advanced Research on Diagnostic Assays (CARDA), Icahn School of Medicine at Mount Sinai, New York, New York, 10029, USA. stuart.sealfon@mssm.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31937892

Citation

Rubenstein, Aliza B., et al. "Single-cell Transcriptional Profiles in Human Skeletal Muscle." Scientific Reports, vol. 10, no. 1, 2020, p. 229.
Rubenstein AB, Smith GR, Raue U, et al. Single-cell transcriptional profiles in human skeletal muscle. Sci Rep. 2020;10(1):229.
Rubenstein, A. B., Smith, G. R., Raue, U., Begue, G., Minchev, K., Ruf-Zamojski, F., ... Sealfon, S. C. (2020). Single-cell transcriptional profiles in human skeletal muscle. Scientific Reports, 10(1), p. 229. doi:10.1038/s41598-019-57110-6.
Rubenstein AB, et al. Single-cell Transcriptional Profiles in Human Skeletal Muscle. Sci Rep. 2020 Jan 14;10(1):229. PubMed PMID: 31937892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-cell transcriptional profiles in human skeletal muscle. AU - Rubenstein,Aliza B, AU - Smith,Gregory R, AU - Raue,Ulrika, AU - Begue,Gwénaëlle, AU - Minchev,Kiril, AU - Ruf-Zamojski,Frederique, AU - Nair,Venugopalan D, AU - Wang,Xingyu, AU - Zhou,Lan, AU - Zaslavsky,Elena, AU - Trappe,Todd A, AU - Trappe,Scott, AU - Sealfon,Stuart C, Y1 - 2020/01/14/ PY - 2019/06/03/received PY - 2019/12/18/accepted PY - 2020/1/16/entrez PY - 2020/1/16/pubmed PY - 2020/1/16/medline SP - 229 EP - 229 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - Skeletal muscle is a heterogeneous tissue comprised of muscle fiber and mononuclear cell types that, in addition to movement, influences immunity, metabolism and cognition. We investigated the gene expression patterns of skeletal muscle cells using RNA-seq of subtype-pooled single human muscle fibers and single cell RNA-seq of mononuclear cells from human vastus lateralis, mouse quadriceps, and mouse diaphragm. We identified 11 human skeletal muscle mononuclear cell types, including two fibro-adipogenic progenitor (FAP) cell subtypes. The human FBN1+ FAP cell subtype is novel and a corresponding FBN1+ FAP cell type was also found in single cell RNA-seq analysis in mouse. Transcriptome exercise studies using bulk tissue analysis do not resolve changes in individual cell-type proportion or gene expression. The cell-type gene signatures provide the means to use computational methods to identify cell-type level changes in bulk studies. As an example, we analyzed public transcriptome data from an exercise training study and revealed significant changes in specific mononuclear cell-type proportions related to age, sex, acute exercise and training. Our single-cell expression map of skeletal muscle cell types will further the understanding of the diverse effects of exercise and the pathophysiology of muscle disease. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31937892/Single-cell_transcriptional_profiles_in_human_skeletal_muscle L2 - http://dx.doi.org/10.1038/s41598-019-57110-6 DB - PRIME DP - Unbound Medicine ER -