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Expression of Wnt/β-catenin related genes after skeletal muscle contusion.
Int J Clin Exp Pathol 2018; 11(2):704-711IJ

Abstract

BACKGROUND

It was aimed to determine expressions of genes related to Wnt/β-catenin signaling for evaluating time duration after skeletal muscle contusion.

METHODS

Pathological change of skeletal muscle was observed after H-E staining. mRNA of respective genes was quantified with real-time quantitative PCR. Expression of β-catenin was further characterized with immunostaining and quantified as intensity/area and further immune blotting and quantified as grey intensity normalized to loading control (GADPH).

RESULTS

After injury, skeletal muscle exhibited prominent inflammatory response, hyperplasia and regeneration. Infiltration of inflammatory cell, formation of myotube and maturation of skeletal muscle fiber were observed under HE staining. Expression of FZD4, Myo D, Myf5 changed during early stages after injury and could serve to evaluate injury within 24 h; Expression of SFRP5 and Fra1 changed during early-to-intermediate stages after injury and could serve to evaluate injury within 12-48 h; Expression of MRF4 changed during intermediate stages after injury and could serve to evaluate injury within 36-48 h; Expression of β-catenin changed during intermediate stages after injury and could serve to evaluate injury within 36 h-3 d; Expression of MyoG changed during late stages after injury and could serve to evaluate injury within 48 h-7 d. Immunostaining experiments showed that 36 h after injury, membrane β-catenin decreased while nucleus β-catenin increased.

CONCLUSION

Wnt/β-catenin related genes are involved in regeneration of skeletal muscle after contusion. The sequential changes of gene expression can be used for evaluating the duration after contusion.

Authors+Show Affiliations

School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.Shanxi Province Public Security Bureau Taiyuan, Shanxi, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.School of Forensic Medicine, Shanxi Medical University Taiyuan 030001, Shanxi Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31938156

Citation

Liang, Xin-Hua, et al. "Expression of Wnt/β-catenin Related Genes After Skeletal Muscle Contusion." International Journal of Clinical and Experimental Pathology, vol. 11, no. 2, 2018, pp. 704-711.
Liang XH, Liu ZJ, Sun JH, et al. Expression of Wnt/β-catenin related genes after skeletal muscle contusion. Int J Clin Exp Pathol. 2018;11(2):704-711.
Liang, X. H., Liu, Z. J., Sun, J. H., Dong, Z. X., Lu, J., Jiang, M. L., ... Wang, Y. Y. (2018). Expression of Wnt/β-catenin related genes after skeletal muscle contusion. International Journal of Clinical and Experimental Pathology, 11(2), pp. 704-711.
Liang XH, et al. Expression of Wnt/β-catenin Related Genes After Skeletal Muscle Contusion. Int J Clin Exp Pathol. 2018;11(2):704-711. PubMed PMID: 31938156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Wnt/β-catenin related genes after skeletal muscle contusion. AU - Liang,Xin-Hua, AU - Liu,Zhi-Jie, AU - Sun,Jun-Hong, AU - Dong,Zu-Xin, AU - Lu,Jian, AU - Jiang,Mei-Ling, AU - Wang,Li-Xiao, AU - Wang,Ying-Yuan, Y1 - 2018/02/01/ PY - 2017/07/30/received PY - 2017/12/12/accepted PY - 2020/1/16/entrez PY - 2018/2/1/pubmed PY - 2018/2/1/medline KW - Forensic pathology KW - Wnt signaling pathway KW - injury time estimation KW - skeletal muscle contusion KW - skeletal muscle satellite cell SP - 704 EP - 711 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 11 IS - 2 N2 - BACKGROUND: It was aimed to determine expressions of genes related to Wnt/β-catenin signaling for evaluating time duration after skeletal muscle contusion. METHODS: Pathological change of skeletal muscle was observed after H-E staining. mRNA of respective genes was quantified with real-time quantitative PCR. Expression of β-catenin was further characterized with immunostaining and quantified as intensity/area and further immune blotting and quantified as grey intensity normalized to loading control (GADPH). RESULTS: After injury, skeletal muscle exhibited prominent inflammatory response, hyperplasia and regeneration. Infiltration of inflammatory cell, formation of myotube and maturation of skeletal muscle fiber were observed under HE staining. Expression of FZD4, Myo D, Myf5 changed during early stages after injury and could serve to evaluate injury within 24 h; Expression of SFRP5 and Fra1 changed during early-to-intermediate stages after injury and could serve to evaluate injury within 12-48 h; Expression of MRF4 changed during intermediate stages after injury and could serve to evaluate injury within 36-48 h; Expression of β-catenin changed during intermediate stages after injury and could serve to evaluate injury within 36 h-3 d; Expression of MyoG changed during late stages after injury and could serve to evaluate injury within 48 h-7 d. Immunostaining experiments showed that 36 h after injury, membrane β-catenin decreased while nucleus β-catenin increased. CONCLUSION: Wnt/β-catenin related genes are involved in regeneration of skeletal muscle after contusion. The sequential changes of gene expression can be used for evaluating the duration after contusion. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/31938156/Expression_of_Wnt/β-catenin_related_genes_after_skeletal_muscle_contusion L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31938156/ DB - PRIME DP - Unbound Medicine ER -