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Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis.
Pathogens. 2020 Jan 11; 9(1)P

Abstract

Although the available proteomic studies have made it possible to identify and characterize Trichinella stage-specific proteins reacting with infected host-specific antibodies, the vast majority of these studies do not provide any information about changes in the global proteomic serum profile of Trichinella-infested individuals. In view of the above, the present study aimed to examine the protein expression profile of serum obtained at 13 and 60 days postinfection (d.p.i.) from three groups of pigs experimentally infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis and from uninfected, control pigs by two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The comparative proteomic analysis of the T. spiralis group vs the control group revealed 5 differently expressed spots at both 13 and 60 d.p.i. Experimental infection with T. britovi induced significant expression changes in 3 protein spots at 13 d.p.i. and in 6 protein spots at 60 d.p.i. in comparison with the control group. Paired analyses between the group infected with T. pseudospiralis and the uninfected control group revealed 6 differently changed spots at 13 d.p.i. and 2 differently changed spots at 60 d.p.i. Among these 27 spots, 15 were successfully identified. Depending on the Trichinella species triggering the infection and the time point of serum collection, they were IgM heavy-chain constant region, antithrombin III-precursor, immunoglobulin gamma-chain, clusterin, homeobox protein Mohawk, apolipoprotein E precursor, serum amyloid P-component precursor, Ig lambda chains, complement C3 isoform X1, and apolipoprotein A-I. Our results demonstrate that various Trichinella species and different phases of the invasion produce a distinct, characteristic proteomic pattern in the serum of experimentally infected pigs.

Authors+Show Affiliations

Department of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, Lublin 20-950, Poland.Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Klemensa Janickiego 29, Szczecin 71-270, Poland.Department of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, Lublin 20-950, Poland.Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Klemensa Janickiego 29, Szczecin 71-270, Poland.Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Klemensa Janickiego 29, Szczecin 71-270, Poland.Department of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, Lublin 20-950, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31940868

Citation

Gondek, Michał, et al. "Comparative Proteomic Analysis of Serum From Pigs Experimentally Infected With Trichinella Spiralis, Trichinella Britovi, and Trichinella Pseudospiralis." Pathogens (Basel, Switzerland), vol. 9, no. 1, 2020.
Gondek M, Herosimczyk A, Knysz P, et al. Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis. Pathogens. 2020;9(1).
Gondek, M., Herosimczyk, A., Knysz, P., Ożgo, M., Lepczyński, A., & Szkucik, K. (2020). Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis. Pathogens (Basel, Switzerland), 9(1). https://doi.org/10.3390/pathogens9010055
Gondek M, et al. Comparative Proteomic Analysis of Serum From Pigs Experimentally Infected With Trichinella Spiralis, Trichinella Britovi, and Trichinella Pseudospiralis. Pathogens. 2020 Jan 11;9(1) PubMed PMID: 31940868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis. AU - Gondek,Michał, AU - Herosimczyk,Agnieszka, AU - Knysz,Przemysław, AU - Ożgo,Małgorzata, AU - Lepczyński,Adam, AU - Szkucik,Krzysztof, Y1 - 2020/01/11/ PY - 2019/12/02/received PY - 2020/01/05/revised PY - 2020/01/09/accepted PY - 2020/1/17/entrez KW - 2-DE KW - MALDI-TOF MS KW - Trichinella spp. KW - experimental infection KW - pigs KW - serum proteomics JF - Pathogens (Basel, Switzerland) JO - Pathogens VL - 9 IS - 1 N2 - Although the available proteomic studies have made it possible to identify and characterize Trichinella stage-specific proteins reacting with infected host-specific antibodies, the vast majority of these studies do not provide any information about changes in the global proteomic serum profile of Trichinella-infested individuals. In view of the above, the present study aimed to examine the protein expression profile of serum obtained at 13 and 60 days postinfection (d.p.i.) from three groups of pigs experimentally infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis and from uninfected, control pigs by two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The comparative proteomic analysis of the T. spiralis group vs the control group revealed 5 differently expressed spots at both 13 and 60 d.p.i. Experimental infection with T. britovi induced significant expression changes in 3 protein spots at 13 d.p.i. and in 6 protein spots at 60 d.p.i. in comparison with the control group. Paired analyses between the group infected with T. pseudospiralis and the uninfected control group revealed 6 differently changed spots at 13 d.p.i. and 2 differently changed spots at 60 d.p.i. Among these 27 spots, 15 were successfully identified. Depending on the Trichinella species triggering the infection and the time point of serum collection, they were IgM heavy-chain constant region, antithrombin III-precursor, immunoglobulin gamma-chain, clusterin, homeobox protein Mohawk, apolipoprotein E precursor, serum amyloid P-component precursor, Ig lambda chains, complement C3 isoform X1, and apolipoprotein A-I. Our results demonstrate that various Trichinella species and different phases of the invasion produce a distinct, characteristic proteomic pattern in the serum of experimentally infected pigs. SN - 2076-0817 UR - https://www.unboundmedicine.com/medline/citation/31940868/Comparative_Proteomic_Analysis_of_Serum_from_Pigs_Experimentally_Infected_with_Trichinella_spiralis,_Trichinella_britovi,_and_Trichinella_pseudospiralis L2 - http://www.mdpi.com/resolver?pii=pathogens9010055 DB - PRIME DP - Unbound Medicine ER -
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