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Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410.
Int J Mol Sci. 2020 Jan 12; 21(2)IJ

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5-F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.

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Authors+Show Affiliations

Liu Y
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.
Qie Y
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, 162 Hengshan Street, Gaoxin District, Shijiazhuang, Hebei 050035, China.
Li X
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071001, China.
Wang M
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.
Chen X
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA 99164-6430, USA.

MeSH

BasidiomycotaChromosome MappingChromosomes, PlantDisease ResistanceHot TemperaturePlant BreedingPlant LeavesPolymorphism, Single NucleotideQuantitative Trait LociTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31940871

Citation

Liu, Yan, et al. "Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410." International Journal of Molecular Sciences, vol. 21, no. 2, 2020.
Liu Y, Qie Y, Li X, et al. Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410. Int J Mol Sci. 2020;21(2).
Liu, Y., Qie, Y., Li, X., Wang, M., & Chen, X. (2020). Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410. International Journal of Molecular Sciences, 21(2). https://doi.org/10.3390/ijms21020478
Liu Y, et al. Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410. Int J Mol Sci. 2020 Jan 12;21(2) PubMed PMID: 31940871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410. AU - Liu,Yan, AU - Qie,Yanmin, AU - Li,Xing, AU - Wang,Meinan, AU - Chen,Xianming, Y1 - 2020/01/12/ PY - 2019/12/20/received PY - 2020/01/08/revised PY - 2020/01/09/accepted PY - 2020/1/17/entrez PY - 2020/1/17/pubmed PY - 2020/6/4/medline KW - Puccinia striiformis KW - QTL mapping KW - Triticum aestivum KW - disease resistance KW - stripe rust KW - wheat JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 2 N2 - Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5-F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31940871/Genome_Wide_Mapping_of_Quantitative_Trait_Loci_Conferring_All_Stage_and_High_Temperature_Adult_Plant_Resistance_to_Stripe_Rust_in_Spring_Wheat_Landrace_PI_181410_ DB - PRIME DP - Unbound Medicine ER -