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Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS.
Eur J Histochem 2020; 64(1)EJ

Abstract

The seed morphology of three Pseudocereal Grains (PSCg), i.e. quinoa (Chenopodium quinoa Willd, Chenopodiaceae), buckwheat (Fagopyrum esculentum Moench, Polygonaceae) and amaranth (Amaranthus caudatus L., Amaranthaceae) was studied by light microscopy (LM) and Environmental Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (ESEM-EDS). LM was used with visible light to evaluate either unstained sections or sections stained with Azan mixture and with fluorescent light. The aim of the study was to compare the architecture of the three seeds in order to connect their morphology with nutrient localization. The Azan staining allowed for the visualization of the seed coat, the embryo - with its shoot apical meristem - and the radicle cell layers, whereas the use of fluorescent microscopy identified the cells rich in phenolic compounds. Finally, the ESEM-EDS analysis revealed that the seed coat of the quinoa was thinner than that of amaranth or buckwheat. In all PSCg, starch granules appeared to be located in large polygonal cells, surrounded by a thin cell wall. Several globoids of proteins were observed in the embryo cells. In the radicle section, the vascular bundles of the procambium were evident, while Amaranth only showed a consistent layer of calcium crystals, located between the embryo and the perysperm. The morphological differences of the three PSCg were discussed in the context of their structural resistance to processing technologies which impact on nutritional value of derived foods.

Authors+Show Affiliations

Department of Biomolecular Sciences, University of Urbino Carlo Bo. paolino.ninfali@uniurb.it.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31941265

Citation

Ninfali, Paolino, et al. "Morphological Analysis of the Seeds of Three Pseudocereals By Using Light Microscopy and ESEM-EDS." European Journal of Histochemistry : EJH, vol. 64, no. 1, 2020.
Ninfali P, Panato A, Bortolotti F, et al. Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS. Eur J Histochem. 2020;64(1).
Ninfali, P., Panato, A., Bortolotti, F., Valentini, L., & Gobbi, P. (2020). Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS. European Journal of Histochemistry : EJH, 64(1), doi:10.4081/ejh.2020.3075.
Ninfali P, et al. Morphological Analysis of the Seeds of Three Pseudocereals By Using Light Microscopy and ESEM-EDS. Eur J Histochem. 2020 Jan 10;64(1) PubMed PMID: 31941265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS. AU - Ninfali,Paolino, AU - Panato,Anna, AU - Bortolotti,Federica, AU - Valentini,Laura, AU - Gobbi,Pietro, Y1 - 2020/01/10/ PY - 2019/10/12/received PY - 2019/12/30/accepted PY - 2020/1/17/entrez JF - European journal of histochemistry : EJH JO - Eur J Histochem VL - 64 IS - 1 N2 - The seed morphology of three Pseudocereal Grains (PSCg), i.e. quinoa (Chenopodium quinoa Willd, Chenopodiaceae), buckwheat (Fagopyrum esculentum Moench, Polygonaceae) and amaranth (Amaranthus caudatus L., Amaranthaceae) was studied by light microscopy (LM) and Environmental Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (ESEM-EDS). LM was used with visible light to evaluate either unstained sections or sections stained with Azan mixture and with fluorescent light. The aim of the study was to compare the architecture of the three seeds in order to connect their morphology with nutrient localization. The Azan staining allowed for the visualization of the seed coat, the embryo - with its shoot apical meristem - and the radicle cell layers, whereas the use of fluorescent microscopy identified the cells rich in phenolic compounds. Finally, the ESEM-EDS analysis revealed that the seed coat of the quinoa was thinner than that of amaranth or buckwheat. In all PSCg, starch granules appeared to be located in large polygonal cells, surrounded by a thin cell wall. Several globoids of proteins were observed in the embryo cells. In the radicle section, the vascular bundles of the procambium were evident, while Amaranth only showed a consistent layer of calcium crystals, located between the embryo and the perysperm. The morphological differences of the three PSCg were discussed in the context of their structural resistance to processing technologies which impact on nutritional value of derived foods. SN - 2038-8306 UR - https://www.unboundmedicine.com/medline/citation/31941265/Morphological_analysis_of_the_seeds_of_three_pseudocereals_by_using_light_microscopy_and_ESEM-EDS L2 - https://doi.org/10.4081/ejh.2020.3075 DB - PRIME DP - Unbound Medicine ER -