Diversity and space-time dynamics of the bacterial communities in cotton (Gossypium hirsutum) rhizosphere soil.
Can J Microbiol. 2020 Mar; 66(3):228-242.CJ

Abstract

Rhizosphere bacteria are key determinants of plant health and productivity. In this study, we used PCR-based next-generation sequencing to reveal the diversity and community composition of bacteria in the cotton rhizosphere from samples collected in Xinjiang Province, China. We identified 125 bacterial classes within 49 phyla from these samples. Proteobacteria (33.07% of total sequences), Acidobacteria (19.88%), and Gemmatimonadetes (11.19%) dominated the bacterial community. Marked differences were evident in the α-diversity of rhizosphere bacteria during different cotton plant growth and development stages. The operational taxonomic unit (OTU) numbers were highest in seedling and bud stages and decreased at the flowering and fruit-boll-opening stages. Forty-three OTUs from the Proteobacteria were common to all four periods of cotton development. Proteobacteria were more abundant in the rhizospheres of cotton from southern Xinjiang than from northern Xinjiang, while the opposite trend was observed for Acidobacteria. Gemmatimonadetes frequency was broadly the same in both northern and southern Xinjiang. These results suggest that there is abundant diversity in the microbiota of cotton rhizosphere soil. Proteobacteria and Actinobacteria dominated this microbial niche and bacterial communities in the seedling, bud, flowering, and boll-opening stages appear to be more similar to one another than to communities at the other growth stages.

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Authors+Show Affiliations

Shi Y

Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P.R. China. Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P.R. China.

Yang H

Chu M

Niu X

Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P.R. China.

MeSH

BacteriaBiodiversityChinaGossypiumHigh-Throughput Nucleotide SequencingMicrobiotaRhizosphereSoilSoil Microbiology

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31944857

Citation

Shi, YingWu, et al. "Diversity and Space-time Dynamics of the Bacterial Communities in Cotton (Gossypium Hirsutum) Rhizosphere Soil." Canadian Journal of Microbiology, vol. 66, no. 3, 2020, pp. 228-242.

Shi Y, Yang H, Chu M, et al. Diversity and space-time dynamics of the bacterial communities in cotton (Gossypium hirsutum) rhizosphere soil. Can J Microbiol. 2020;66(3):228-242.

Shi, Y., Yang, H., Chu, M., Niu, X., Huo, X., Gao, Y., Zeng, J., Lin, Q., Zhang, T., Li, Y., Outi, K., Lou, K., Li, X., Dang, W., & Zhang, T. (2020). Diversity and space-time dynamics of the bacterial communities in cotton (Gossypium hirsutum) rhizosphere soil. Canadian Journal of Microbiology, 66(3), 228-242. https://doi.org/10.1139/cjm-2019-0196

Shi Y, et al. Diversity and Space-time Dynamics of the Bacterial Communities in Cotton (Gossypium Hirsutum) Rhizosphere Soil. Can J Microbiol. 2020;66(3):228-242. PubMed PMID: 31944857.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Diversity and space-time dynamics of the bacterial communities in cotton (Gossypium hirsutum) rhizosphere soil. AU - Shi,YingWu, AU - Yang,HongMei, AU - Chu,Ming, AU - Niu,XinXiang, AU - Huo,XiangDong, AU - Gao,Yan, AU - Zeng,Jun, AU - Lin,Qing, AU - Zhang,Tao, AU - Li,YuGuo, AU - Outi,KuEr, AU - Lou,Kai, AU - Li,XueYan, AU - Dang,WenFang, AU - Zhang,Tao, Y1 - 2020/01/16/ PY - 2020/1/17/pubmed PY - 2020/5/19/medline PY - 2020/1/17/entrez KW - 16S rRNA gene KW - Gossypium hirsutum KW - Illumina MiSeq KW - bactéries de la rhizosphère KW - biodiversity KW - biodiversité KW - biogeography KW - biogéographie KW - growth stage KW - gène de l’ARNr 16S KW - rhizosphere bacteria KW - stade de croissance SP - 228 EP - 242 JF - Canadian journal of microbiology JO - Can J Microbiol VL - 66 IS - 3 N2 - Rhizosphere bacteria are key determinants of plant health and productivity. In this study, we used PCR-based next-generation sequencing to reveal the diversity and community composition of bacteria in the cotton rhizosphere from samples collected in Xinjiang Province, China. We identified 125 bacterial classes within 49 phyla from these samples. Proteobacteria (33.07% of total sequences), Acidobacteria (19.88%), and Gemmatimonadetes (11.19%) dominated the bacterial community. Marked differences were evident in the α-diversity of rhizosphere bacteria during different cotton plant growth and development stages. The operational taxonomic unit (OTU) numbers were highest in seedling and bud stages and decreased at the flowering and fruit-boll-opening stages. Forty-three OTUs from the Proteobacteria were common to all four periods of cotton development. Proteobacteria were more abundant in the rhizospheres of cotton from southern Xinjiang than from northern Xinjiang, while the opposite trend was observed for Acidobacteria. Gemmatimonadetes frequency was broadly the same in both northern and southern Xinjiang. These results suggest that there is abundant diversity in the microbiota of cotton rhizosphere soil. Proteobacteria and Actinobacteria dominated this microbial niche and bacterial communities in the seedling, bud, flowering, and boll-opening stages appear to be more similar to one another than to communities at the other growth stages. SN - 1480-3275 UR - https://www.unboundmedicine.com/medline/citation/31944857/Diversity_and_space_time_dynamics_of_the_bacterial_communities_in_cotton__Gossypium_hirsutum__rhizosphere_soil_ DB - PRIME DP - Unbound Medicine ER -

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Diversity and space-time dynamics of the bacterial communities in cotton (Gossypium hirsutum) rhizosphere soil.Can J Microbiol. 2020 Mar; 66(3):228-242.CJ