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Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii.
Int J Mol Sci. 2020 Jan 03; 21(1)IJ

Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

Authors+Show Affiliations

National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007, USA.Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31947730

Citation

Dong, Zhenjie, et al. "Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived From Aegilops Searsii." International Journal of Molecular Sciences, vol. 21, no. 1, 2020.
Dong Z, Tian X, Ma C, et al. Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii. Int J Mol Sci. 2020;21(1).
Dong, Z., Tian, X., Ma, C., Xia, Q., Wang, B., Chen, Q., Sehgal, S. K., Friebe, B., Li, H., & Liu, W. (2020). Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii. International Journal of Molecular Sciences, 21(1). https://doi.org/10.3390/ijms21010322
Dong Z, et al. Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived From Aegilops Searsii. Int J Mol Sci. 2020 Jan 3;21(1) PubMed PMID: 31947730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii. AU - Dong,Zhenjie, AU - Tian,Xiubin, AU - Ma,Chao, AU - Xia,Qing, AU - Wang,Beilin, AU - Chen,Qifan, AU - Sehgal,Sunish K, AU - Friebe,Bernd, AU - Li,Huanhuan, AU - Liu,Wenxuan, Y1 - 2020/01/03/ PY - 2019/10/30/received PY - 2019/12/21/revised PY - 2019/12/31/accepted PY - 2020/1/18/entrez PY - 2020/1/18/pubmed PY - 2020/9/9/medline KW - Pm57 KW - RNA-seq KW - common wheat KW - molecular markers KW - physical mapping JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 1 N2 - Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31947730/Physical_Mapping_of_Pm57_a_Powdery_Mildew_Resistance_Gene_Derived_from_Aegilops_searsii_ L2 - https://www.mdpi.com/resolver?pii=ijms21010322 DB - PRIME DP - Unbound Medicine ER -