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Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus communis L.).
Int J Mol Sci. 2020 Jan 15; 21(2)IJ

Abstract

Cellular autophagy is a widely-occurring conserved process for turning over damaged organelles or recycling cytoplasmic contents in cells. Although autophagy-related genes (ATGs) have been broadly identified from many plants, little is known about the potential function of autophagy in mediating plant growth and development, particularly in recycling cytoplasmic contents during seed development and germination. Castor bean (Ricinus communis) is one of the most important inedible oilseed crops. Its mature seed has a persistent and large endosperm with a hard and lignified seed coat, and is considered a model system for studying seed biology. Here, a total of 34 RcATG genes were identified in the castor bean genome and their sequence structures were characterized. The expressional profiles of these RcATGs were examined using RNA-seq and real-time PCR in a variety of tissues. In particular, we found that most RcATGs were significantly up-regulated in the later stage of seed coat development, tightly associated with the lignification of cell wall tissues. During seed germination, the expression patterns of most RcATGs were associated with the decomposition of storage oils. Furthermore, we observed by electron microscopy that the lipid droplets were directly swallowed by the vacuoles, suggesting that autophagy directly participates in mediating the decomposition of lipid droplets via the microlipophagy pathway in germinating castor bean seeds. This study provides novel insights into understanding the potential function of autophagy in mediating seed development and germination.

Authors+Show Affiliations

Department of Economic Plants and Biotechnology, and Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China.College Life Sciences, Yunnan University, Kunming 650091, China.College Life Sciences, Yunnan University, Kunming 650091, China.Department of Economic Plants and Biotechnology, and Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.College Life Sciences, Yunnan University, Kunming 650091, China.Key Laboratory for Forest Resources Conservation and Utilization in Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming 650201, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31952322

Citation

Han, Bing, et al. "Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus Communis L.)." International Journal of Molecular Sciences, vol. 21, no. 2, 2020.
Han B, Xu H, Feng Y, et al. Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus communis L.). Int J Mol Sci. 2020;21(2).
Han, B., Xu, H., Feng, Y., Xu, W., Cui, Q., & Liu, A. (2020). Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus communis L.). International Journal of Molecular Sciences, 21(2). https://doi.org/10.3390/ijms21020562
Han B, et al. Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus Communis L.). Int J Mol Sci. 2020 Jan 15;21(2) PubMed PMID: 31952322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (ATGs) in Oilseed Crop Castor Bean (Ricinus communis L.). AU - Han,Bing, AU - Xu,Hui, AU - Feng,Yingting, AU - Xu,Wei, AU - Cui,Qinghua, AU - Liu,Aizhong, Y1 - 2020/01/15/ PY - 2019/12/30/received PY - 2020/01/12/revised PY - 2020/01/14/accepted PY - 2020/1/19/entrez PY - 2020/1/19/pubmed PY - 2020/10/6/medline KW - autophagy KW - castor bean KW - gene expression KW - seed development KW - seed germination JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 2 N2 - Cellular autophagy is a widely-occurring conserved process for turning over damaged organelles or recycling cytoplasmic contents in cells. Although autophagy-related genes (ATGs) have been broadly identified from many plants, little is known about the potential function of autophagy in mediating plant growth and development, particularly in recycling cytoplasmic contents during seed development and germination. Castor bean (Ricinus communis) is one of the most important inedible oilseed crops. Its mature seed has a persistent and large endosperm with a hard and lignified seed coat, and is considered a model system for studying seed biology. Here, a total of 34 RcATG genes were identified in the castor bean genome and their sequence structures were characterized. The expressional profiles of these RcATGs were examined using RNA-seq and real-time PCR in a variety of tissues. In particular, we found that most RcATGs were significantly up-regulated in the later stage of seed coat development, tightly associated with the lignification of cell wall tissues. During seed germination, the expression patterns of most RcATGs were associated with the decomposition of storage oils. Furthermore, we observed by electron microscopy that the lipid droplets were directly swallowed by the vacuoles, suggesting that autophagy directly participates in mediating the decomposition of lipid droplets via the microlipophagy pathway in germinating castor bean seeds. This study provides novel insights into understanding the potential function of autophagy in mediating seed development and germination. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31952322/Genomic_Characterization_and_Expressional_Profiles_of_Autophagy_Related_Genes__ATGs__in_Oilseed_Crop_Castor_Bean__Ricinus_communis_L___ L2 - https://www.mdpi.com/resolver?pii=ijms21020562 DB - PRIME DP - Unbound Medicine ER -