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Refining the genetic architecture of flag leaf glaucousness in wheat.
Theor Appl Genet. 2020 Mar; 133(3):981-991.TA

Abstract

KEY MESSAGE

The cuticle is the plant's barrier against abiotic and biotic stresses, and the deposition of epicuticular wax crystals results in the scattering of light, an effect termed glaucousness. Here, we dissect the genetic architecture of flag leaf glaucousness in wheat toward a future targeted design of the cuticle. The cuticle serves as a barrier that protects plants against abiotic and biotic stresses. Differences in cuticle composition can be detected by the scattering of light on epicuticular wax crystals, which causes a phenotype termed glaucousness. In this study, we dissected the genetic architecture of flag leaf glaucousness in a panel of 1106 wheat cultivars of global origin. We observed a large genotypic variation, but the geographic pattern suggests that other wax layer characteristics besides glaucousness may be important in conferring tolerance to abiotic stresses such as heat and drought. Genome-wide association mapping identified two major quantitative trait loci (QTL) on chromosomes 3A and 2B. The latter corresponds to the W1 locus, but further characterization revealed that it is likely to contain additional QTL. The same holds true for the major QTL on 3A, which was also found to show an epistatic interaction with another locus located a few centiMorgan distal to it. Genome-wide prediction and the identification of a few additional putative QTL revealed that small-effect QTL also contribute to the trait. Collectively, our results illustrate the complexity of the genetic control of flag leaf glaucousness, with additive effects and epistasis, and lay the foundation for the cloning of the underlying genes toward a more targeted design of the cuticle by plant breeding.

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Authors+Show Affiliations

Würschum T
State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany. tobias.wuerschum@uni-hohenheim.de.
Langer SM
State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany. BASF Agricultural Solutions GmbH, Gatersleben, Germany.
Longin CFH
State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany.
Tucker MR
School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, Australia.
Leiser WL
State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany.

MeSH

Chromosome MappingChromosomes, PlantEpistasis, GeneticGene Expression Regulation, PlantGenetic Association StudiesGenome-Wide Association StudyGenotypePhenotypePlant BreedingPlant LeavesQuantitative Trait LociStress, PhysiologicalTriticumWaxes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31953547

Citation

Würschum, Tobias, et al. "Refining the Genetic Architecture of Flag Leaf Glaucousness in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 3, 2020, pp. 981-991.
Würschum T, Langer SM, Longin CFH, et al. Refining the genetic architecture of flag leaf glaucousness in wheat. Theor Appl Genet. 2020;133(3):981-991.
Würschum, T., Langer, S. M., Longin, C. F. H., Tucker, M. R., & Leiser, W. L. (2020). Refining the genetic architecture of flag leaf glaucousness in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(3), 981-991. https://doi.org/10.1007/s00122-019-03522-x
Würschum T, et al. Refining the Genetic Architecture of Flag Leaf Glaucousness in Wheat. Theor Appl Genet. 2020;133(3):981-991. PubMed PMID: 31953547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Refining the genetic architecture of flag leaf glaucousness in wheat. AU - Würschum,Tobias, AU - Langer,Simon M, AU - Longin,C Friedrich H, AU - Tucker,Matthew R, AU - Leiser,Willmar L, Y1 - 2020/01/17/ PY - 2019/09/25/received PY - 2019/12/20/accepted PY - 2020/1/19/pubmed PY - 2020/11/20/medline PY - 2020/1/19/entrez SP - 981 EP - 991 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 3 N2 - KEY MESSAGE: The cuticle is the plant's barrier against abiotic and biotic stresses, and the deposition of epicuticular wax crystals results in the scattering of light, an effect termed glaucousness. Here, we dissect the genetic architecture of flag leaf glaucousness in wheat toward a future targeted design of the cuticle. The cuticle serves as a barrier that protects plants against abiotic and biotic stresses. Differences in cuticle composition can be detected by the scattering of light on epicuticular wax crystals, which causes a phenotype termed glaucousness. In this study, we dissected the genetic architecture of flag leaf glaucousness in a panel of 1106 wheat cultivars of global origin. We observed a large genotypic variation, but the geographic pattern suggests that other wax layer characteristics besides glaucousness may be important in conferring tolerance to abiotic stresses such as heat and drought. Genome-wide association mapping identified two major quantitative trait loci (QTL) on chromosomes 3A and 2B. The latter corresponds to the W1 locus, but further characterization revealed that it is likely to contain additional QTL. The same holds true for the major QTL on 3A, which was also found to show an epistatic interaction with another locus located a few centiMorgan distal to it. Genome-wide prediction and the identification of a few additional putative QTL revealed that small-effect QTL also contribute to the trait. Collectively, our results illustrate the complexity of the genetic control of flag leaf glaucousness, with additive effects and epistasis, and lay the foundation for the cloning of the underlying genes toward a more targeted design of the cuticle by plant breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31953547/Refining_the_genetic_architecture_of_flag_leaf_glaucousness_in_wheat_ DB - PRIME DP - Unbound Medicine ER -