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Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat.
Theor Appl Genet. 2020 Apr; 133(4):1095-1107.TA

Abstract

KEY MESSAGE

A physical map of Secale cereale chromosome 6R was constructed using deletion mapping, and a new stripe rust resistance gene Yr83 was mapped to the deletion bin of FL 0.73-1.00 of 6RL. Rye (Secale cereale L., RR) possesses valuable genes for wheat improvement. In the current study, we report a resistance gene conferring stripe rust resistance effective from seedling to adult plant stages located on chromosome 6R. This chromosome was derived from triticale line T-701 and also carries highly effective resistance to the cereal cyst nematode species Heterodera avenae Woll. A wheat-rye 6R(6D) disomic substitution line exhibited high levels of seedling resistance to Australian pathotypes of the stripe rust (Puccinia striiformis f. sp. tritici; Pst) pathogen and showed an even greater resistance to the Chinese Pst pathotypes in the field. Ten chromosome 6R deletion lines and five wheat-rye 6R translocation lines were developed earlier in the attempt to transfer the nematode resistance gene to wheat and used herein to map the stripe rust resistance gene. These lines were subsequently characterized by sequential multicolor fluorescence in situ hybridization (mc-FISH), genomic in situ hybridization (GISH), mc-GISH, PCR-based landmark unique gene (PLUG), and chromosome 6R-specific length amplified fragment sequencing (SLAF-Seq) marker analyses to physically map the stripe rust resistance gene. The new stripe rust resistance locus was located in a chromosomal bin with fraction length (FL) 0.73-1.00 on 6RL and was named Yr83. A wheat-rye translocation line T6RL (#5) carrying the stripe rust resistance gene will be useful as a new germplasm in breeding for resistance.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li J
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.
Dundas I
School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia. ian.dundas@adelaide.edu.au.
Dong C
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Li G
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.
Trethowan R
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Yang Z
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.
Hoxha S
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Zhang P
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. peng.zhang@sydney.edu.au.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceGenes, PlantMetaphasePhysical Chromosome MappingPlant DiseasesPlants, Genetically ModifiedSecaleSeedlingsTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31955232

Citation

Li, Jianbo, et al. "Identification and Characterization of a New Stripe Rust Resistance Gene Yr83 On Rye Chromosome 6R in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 4, 2020, pp. 1095-1107.
Li J, Dundas I, Dong C, et al. Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. Theor Appl Genet. 2020;133(4):1095-1107.
Li, J., Dundas, I., Dong, C., Li, G., Trethowan, R., Yang, Z., Hoxha, S., & Zhang, P. (2020). Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(4), 1095-1107. https://doi.org/10.1007/s00122-020-03534-y
Li J, et al. Identification and Characterization of a New Stripe Rust Resistance Gene Yr83 On Rye Chromosome 6R in Wheat. Theor Appl Genet. 2020;133(4):1095-1107. PubMed PMID: 31955232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. AU - Li,Jianbo, AU - Dundas,Ian, AU - Dong,Chongmei, AU - Li,Guangrong, AU - Trethowan,Richard, AU - Yang,Zujun, AU - Hoxha,Sami, AU - Zhang,Peng, Y1 - 2020/01/18/ PY - 2019/08/14/received PY - 2020/01/03/accepted PY - 2020/1/20/pubmed PY - 2020/11/11/medline PY - 2020/1/20/entrez SP - 1095 EP - 1107 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 4 N2 - KEY MESSAGE: A physical map of Secale cereale chromosome 6R was constructed using deletion mapping, and a new stripe rust resistance gene Yr83 was mapped to the deletion bin of FL 0.73-1.00 of 6RL. Rye (Secale cereale L., RR) possesses valuable genes for wheat improvement. In the current study, we report a resistance gene conferring stripe rust resistance effective from seedling to adult plant stages located on chromosome 6R. This chromosome was derived from triticale line T-701 and also carries highly effective resistance to the cereal cyst nematode species Heterodera avenae Woll. A wheat-rye 6R(6D) disomic substitution line exhibited high levels of seedling resistance to Australian pathotypes of the stripe rust (Puccinia striiformis f. sp. tritici; Pst) pathogen and showed an even greater resistance to the Chinese Pst pathotypes in the field. Ten chromosome 6R deletion lines and five wheat-rye 6R translocation lines were developed earlier in the attempt to transfer the nematode resistance gene to wheat and used herein to map the stripe rust resistance gene. These lines were subsequently characterized by sequential multicolor fluorescence in situ hybridization (mc-FISH), genomic in situ hybridization (GISH), mc-GISH, PCR-based landmark unique gene (PLUG), and chromosome 6R-specific length amplified fragment sequencing (SLAF-Seq) marker analyses to physically map the stripe rust resistance gene. The new stripe rust resistance locus was located in a chromosomal bin with fraction length (FL) 0.73-1.00 on 6RL and was named Yr83. A wheat-rye translocation line T6RL (#5) carrying the stripe rust resistance gene will be useful as a new germplasm in breeding for resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31955232/Identification_and_characterization_of_a_new_stripe_rust_resistance_gene_Yr83_on_rye_chromosome_6R_in_wheat_ DB - PRIME DP - Unbound Medicine ER -