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Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation.
FASEB J. 2020 Mar; 34(3):3983-3995.FJ

Abstract

BACKGROUND AND AIMS

Intestinal adaptation in short bowel syndrome (SBS) includes morphologic processes and functional mechanisms. This study investigated whether digestive enzyme expression in the duodenum and colon is upregulated in SBS patients.

METHOD

Sucrase-isomaltase (SI), lactase-phlorizin hydrolase (LPH), and neutral Aminopeptidase N (ApN) were analyzed in duodenal and colonic biopsies from nine SBS patients in a late stage of adaptation as well as healthy and disease controls by immunoelectron microscopy (IEM), Western blots, and enzyme activities. Furthermore, proliferation rates and intestinal microbiota were analyzed in the mucosal specimen.

RESULTS

We found significantly increased amounts of SI, LPH, and ApN in colonocytes in most SBS patients with large variation and strongest effect for SI and ApN. Digestive enzyme expression was only partially elevated in duodenal enterocytes due to a low proliferation level measured by Ki-67 staining. Microbiome analysis revealed high amounts of Lactobacillus resp. low amounts of Proteobacteria in SBS patients with preservation of colon and ileocecal valve. Colonic expression was associated with a better clinical course in single cases.

CONCLUSION

In SBS patients disaccharidases and peptidases can be upregulated in the colon. Stimulation of this colonic intestinalization process by drugs, nutrients, and pre- or probiotics might offer better therapeutic approaches.

Authors+Show Affiliations

Department of Paediatrics, Justus-Liebig-University Giessen, Giessen, Germany.Department of Pathology, Justus-Liebig-University Giessen, Giessen, Germany.Department of Pediatric Surgery, UKE: University Hospital Eppendorf, Altona Children's Hospital, Hamburg, Germany.Department of Pediatric Surgery, Medical Faculty Mannheim, University Heidelberg, Mannheim, Germany.Department of Paediatrics, Justus-Liebig-University Giessen, Giessen, Germany.Department of Paediatrics, Justus-Liebig-University Giessen, Giessen, Germany.Department of Internal Medicine A, Ernst-Moritz-Arndt University, Greifswald, Germany.Institute of Medical Microbiology, Justus-Liebig-University Giessen, Giessen, Germany. German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany.Institute of Medical Microbiology, Justus-Liebig-University Giessen, Giessen, Germany. German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany.Institute of Medical Microbiology, Justus-Liebig-University Giessen, Giessen, Germany. German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany.Department of Paediatrics, Justus-Liebig-University Giessen, Giessen, Germany.Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX, USA.Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany.Department of Paediatrics, Justus-Liebig-University Giessen, Giessen, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31957074

Citation

de Laffolie, Jan, et al. "Digestive Enzyme Expression in the Large Intestine of Children With Short Bowel Syndrome in a Late Stage of Adaptation." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 34, no. 3, 2020, pp. 3983-3995.
de Laffolie J, Sheridan D, Reinshagen K, et al. Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation. FASEB J. 2020;34(3):3983-3995.
de Laffolie, J., Sheridan, D., Reinshagen, K., Wessel, L., Zimmermann, C., Stricker, S., Lerch, M. M., Weigel, M., Hain, T., Domann, E., Rudloff, S., Nichols, B. L., Naim, H. Y., & Zimmer, K. P. (2020). Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 34(3), 3983-3995. https://doi.org/10.1096/fj.201901758RR
de Laffolie J, et al. Digestive Enzyme Expression in the Large Intestine of Children With Short Bowel Syndrome in a Late Stage of Adaptation. FASEB J. 2020;34(3):3983-3995. PubMed PMID: 31957074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation. AU - de Laffolie,Jan, AU - Sheridan,Diana, AU - Reinshagen,Konrad, AU - Wessel,Lucas, AU - Zimmermann,Christian, AU - Stricker,Sebastian, AU - Lerch,Markus M, AU - Weigel,Markus, AU - Hain,Torsten, AU - Domann,Eugen, AU - Rudloff,Silvia, AU - Nichols,Buford L, AU - Naim,Hassan Y, AU - Zimmer,Klaus-Peter, Y1 - 2020/01/19/ PY - 2019/07/13/received PY - 2019/12/23/revised PY - 2019/12/27/accepted PY - 2020/1/21/pubmed PY - 2020/1/21/medline PY - 2020/1/21/entrez KW - enteral nutrition KW - microbiota KW - parenteral nutrition KW - pediatric intestinal failure KW - pediatric short bowel syndrome KW - prognostic factors SP - 3983 EP - 3995 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J. VL - 34 IS - 3 N2 - BACKGROUND AND AIMS: Intestinal adaptation in short bowel syndrome (SBS) includes morphologic processes and functional mechanisms. This study investigated whether digestive enzyme expression in the duodenum and colon is upregulated in SBS patients. METHOD: Sucrase-isomaltase (SI), lactase-phlorizin hydrolase (LPH), and neutral Aminopeptidase N (ApN) were analyzed in duodenal and colonic biopsies from nine SBS patients in a late stage of adaptation as well as healthy and disease controls by immunoelectron microscopy (IEM), Western blots, and enzyme activities. Furthermore, proliferation rates and intestinal microbiota were analyzed in the mucosal specimen. RESULTS: We found significantly increased amounts of SI, LPH, and ApN in colonocytes in most SBS patients with large variation and strongest effect for SI and ApN. Digestive enzyme expression was only partially elevated in duodenal enterocytes due to a low proliferation level measured by Ki-67 staining. Microbiome analysis revealed high amounts of Lactobacillus resp. low amounts of Proteobacteria in SBS patients with preservation of colon and ileocecal valve. Colonic expression was associated with a better clinical course in single cases. CONCLUSION: In SBS patients disaccharidases and peptidases can be upregulated in the colon. Stimulation of this colonic intestinalization process by drugs, nutrients, and pre- or probiotics might offer better therapeutic approaches. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/31957074/Digestive_enzyme_expression_in_the_large_intestine_of_children_with_short_bowel_syndrome_in_a_late_stage_of_adaptation L2 - https://doi.org/10.1096/fj.201901758RR DB - PRIME DP - Unbound Medicine ER -
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